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Fura-2 AM

🥰Excellent
Catalog No. T18995Cas No. 108964-32-5
Alias Fura-2 Acetoxymethyl ester

Fura-2 AM is a membrane-permeable Ca²⁺ (calcium ion) indicator that is converted to Fura-2 by esterase within cells. Its fluorescence emission wavelength is around 510 nm, with excitation maxima at 340 nm under high calcium concentrations and 380 nm under low calcium concentrations. Calcium ion concentrations can be calculated using the fluorescence intensity ratio.

Fura-2 AM

Fura-2 AM

🥰Excellent
Purity: 98.05%
Catalog No. T18995Alias Fura-2 Acetoxymethyl esterCas No. 108964-32-5
Fura-2 AM is a membrane-permeable Ca²⁺ (calcium ion) indicator that is converted to Fura-2 by esterase within cells. Its fluorescence emission wavelength is around 510 nm, with excitation maxima at 340 nm under high calcium concentrations and 380 nm under low calcium concentrations. Calcium ion concentrations can be calculated using the fluorescence intensity ratio.
Pack SizePriceAvailabilityQuantity
1 mg$328In Stock
5 mg$787In Stock
10 mg$1,070In Stock
25 mg$1,590In Stock
50 mg$2,130In Stock
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Purity:98.05%
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Product Introduction

Bioactivity
Description
Fura-2 AM is a membrane-permeable Ca²⁺ (calcium ion) indicator that is converted to Fura-2 by esterase within cells. Its fluorescence emission wavelength is around 510 nm, with excitation maxima at 340 nm under high calcium concentrations and 380 nm under low calcium concentrations. Calcium ion concentrations can be calculated using the fluorescence intensity ratio.
In vitro
A simplified protocol for staining cortical neurons with the calcium dye fura-2 is as follows:
Cells are loaded with the acetoxymethyl ester of fura-2 (fura-2 AM), which diffuses across the cell membrane and is deesterified by cellular esterases to produce the free acid fura-2. The exact parameters for fura-2 loading vary depending on the cell type. It is recommended to test various conditions by preparing several loading solutions containing various concentrations of fura-2, ranging from 1–4 μM. Cells are incubated in the loading solutions for varying times, ranging from 15 minutes to 2 hours, and the loading volume is tested at both room temperature and 37°C.
1. First, prepare a 1 mM stock solution of fura-2 AM by adding 50 μl of DMSO to a 50 μg vial provided by Invitrogen. It is important to use dry DMSO packaged under nitrogen, and it is necessary to remove the DMSO with a needle by piercing the septum to prevent hydration of the DMSO. After preparing the fura-2 AM solution, store it in a dark, dry place. Fura-2 AM in DMSO is stable for 24 hours at room temperature and for several months at -20°C in a dry container.

2. Aliquot 2 ml of culture medium into 15 ml conical tubes, warm to 37°C, and then add 2 μl of Fura-2 AM stock solution to create a 1 μM Fura-2 AM solution. Vortex the solution vigorously for 1 minute.
3. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with cells to the dish.
4. Incubate the neurons at 37°C in a dark incubator for 30 minutes. Time the incubation period precisely.
5. Prepare a 35 mm dish containing 2 ml of tissue culture medium without Fura-2 AM. Remove the coverslip from the loading solution and place it in a new dish.
6. Mount the coverslip on the imaging chamber. Remove the coverslip from the 35 mm dish and quickly mount it on the chamber, making sure to prevent the cells from drying out. We used an imaging chamber manufactured by Warner Instruments that allows a 10 mm coverslip containing the cells to be mounted on the bottom and a second coverslip to be mounted on the top, forming a sandwich. The two coverslips are secured to the chamber with vacuum grease, and two tubes at either end of the chamber allow solutions to be perfused through the chamber. The input line is connected to a syringe, and the output line is connected to a well, which is evacuated by a suction tube connected to a vacuum trap. [3]
SynonymsFura-2 Acetoxymethyl ester
Chemical Properties
Molecular Weight1001.85
FormulaC44H47N3O24
Cas No.108964-32-5
SmilesO=C(OCOC(=O)C)C=1OC(=NC1)C=2OC=3C=C(C(OCCOC4=CC(=CC=C4N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)C)=CC3C2)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
Relative Density.1.400 g/cm3 (Predicted)
Storage & Solubility Information
Storagekeep away from direct sunlight,The compound is unstable in solution. Please use soon | store at -20°C | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 8 mg/mL (7.99 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM0.9982 mL4.9908 mL9.9815 mL49.9077 mL
5 mM0.1996 mL0.9982 mL1.9963 mL9.9815 mL

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