Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

HOE-S 785026 trihydrochloride

🥰Excellent
Catalog No. T19003Cas No. 2320308-12-9
Alias meta-Hoechst trihydrochloride, Meta-Hoechst trihydrochloride, HOE-S 785026 trihydrochloride (132869-83-1 free base)

HOE-S 785026 trihydrochloride is a live cell labeling dye that binds to nucleic acids by targeting the minor groove in DNA double strands, with a preference for A/T-rich regions (fluorescence is significantly enhanced by A/T-rich double-stranded DNA). It crosses cell membranes and binds to living or immobilized cells, with its fluorescence intensity increasing as the solution pH rises.

HOE-S 785026 trihydrochloride

HOE-S 785026 trihydrochloride

🥰Excellent
Purity: 99.65%
Catalog No. T19003Alias meta-Hoechst trihydrochloride, Meta-Hoechst trihydrochloride, HOE-S 785026 trihydrochloride (132869-83-1 free base)Cas No. 2320308-12-9
HOE-S 785026 trihydrochloride is a live cell labeling dye that binds to nucleic acids by targeting the minor groove in DNA double strands, with a preference for A/T-rich regions (fluorescence is significantly enhanced by A/T-rich double-stranded DNA). It crosses cell membranes and binds to living or immobilized cells, with its fluorescence intensity increasing as the solution pH rises.
Pack SizePriceAvailabilityQuantity
5 mg$47In Stock
10 mg$77In Stock
25 mg$158In Stock
50 mg$253In Stock
100 mg$417In Stock
200 mg$585In Stock
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Select Batch
Purity:99.65%
Contact us for more batch information
Resource Download
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
HOE-S 785026 trihydrochloride is a live cell labeling dye that binds to nucleic acids by targeting the minor groove in DNA double strands, with a preference for A/T-rich regions (fluorescence is significantly enhanced by A/T-rich double-stranded DNA). It crosses cell membranes and binds to living or immobilized cells, with its fluorescence intensity increasing as the solution pH rises.
Cell Research
Instructions
I. Solution preparation
1. Preparation of mother solution: Dissolve HOE-S 785026 trihydrochloride in anhydrous DMSO to prepare a 1 mg/mL mother solution. To ensure complete dissolution, the mother solution can be warmed, but high temperature should be avoided to prevent degradation.
Note: When storing the mother solution, be sure to seal it and store it at -20°C away from light.
2. Preparation of working solution: Dilute the mother solution to the required concentration in an appropriate proportion, usually using a working concentration of 5-10 μg/mL. Use serum-free cell culture medium, PBS or other suitable buffer for dilution. The specific concentration can be adjusted according to experimental needs.
II. Operation steps
1. Cell staining:
Adherent cells: First culture the cells in a culture dish to an appropriate density, add the working solution, and incubate at room temperature for 15-20 minutes.
Suspended cells: Collect the cells by centrifugation, remove the culture medium, add the working solution, and incubate at room temperature for 10-15 minutes.
During the incubation process, ensure that the cells are evenly exposed to the dye solution.
2. Washing steps:
1) After incubation, wash the cells three times with PBS or culture medium, gently centrifuging each time to remove excess dye.
2) Resuspend the cells with fresh culture medium and prepare for fluorescence detection.
3. Fluorescence observation: Use a fluorescence microscope at a wavelength of 350-370 nm for excitation and an emission wavelength of approximately 460-470 nm to observe the staining results.
Aliasmeta-Hoechst trihydrochloride, Meta-Hoechst trihydrochloride, HOE-S 785026 trihydrochloride (132869-83-1 free base)
Chemical Properties
Molecular Weight533.88
FormulaC25H27Cl3N6O
Cas No.2320308-12-9
SmilesOC1=CC=CC(C2=NC3=CC(C4=NC5=CC(N6CCN(C)CC6)=CC=C5N4)=CC=C3N2)=C1.[H]Cl.[H]Cl.[H]Cl
Relative Density.no data available
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
H2O: 47.3 mg/mL (88.60 mM), Sonication and heating are recommended.
Solution Preparation Table
H2O
1mg5mg10mg50mg
1 mM1.8731 mL9.3654 mL18.7308 mL93.6540 mL
5 mM0.3746 mL1.8731 mL3.7462 mL18.7308 mL
10 mM0.1873 mL0.9365 mL1.8731 mL9.3654 mL
20 mM0.0937 mL0.4683 mL0.9365 mL4.6827 mL
50 mM0.0375 mL0.1873 mL0.3746 mL1.8731 mL

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy HOE-S 785026 trihydrochloride | purchase HOE-S 785026 trihydrochloride | HOE-S 785026 trihydrochloride cost | order HOE-S 785026 trihydrochloride | HOE-S 785026 trihydrochloride chemical structure | HOE-S 785026 trihydrochloride formula | HOE-S 785026 trihydrochloride molecular weight