purification

Potassium acetate (Diuretic salt) with antibacterial and antifungal properties.

Edetic acid (Ethylenediaminetetraacetic acid) is a chelating agent commonly used in protein purification, both to eliminate contaminating divalent cations and to inhibit protease activity.

MES monohydrate is an amphoteric ionic buffer that is a PH stabilizing reagent used for protein purification and enzyme assays.

Gel filtration medium G-100, a substance designed for protein purification, enables the separation and purification of proteins through gel filtration.

ZWITTERGENT 3-16 Detergent is a quaternary ammonium surfactant, emulsifier, foaming and wetting agent commonly used in the skin care industry.ZWITTERGENT 3-16 Detergent is involved in protein purification.

Lauroylsarcosine sodium salt(Sarkosyl NL sodium) is an anionic surfactant and detergent with protein denaturing properties commonly used in molecular biology research for purification of membrane proteins, promotion of cell lysis in RNA purification experiments and membrane solubilization, and toxicity to A549 cells after ALIF or SME exposure.

1-Amino-1-deoxy-β-D-galactose (β-D-Galactosylamine) is a galactose analog utilized as a competitive inhibitor. It facilitates the separation, purification, identification, differentiation, and characterization of β-D-galactosidases and galactose oxidases.

Cross-linked dextran G 200 is a hydrophilic gel designed for the separation of macromolecules based on molecular size exclusion. It operates through a gel permeation mechanism; its cross-linked structure forms a three-dimensional network with specific pore sizes, facilitating separation according to the hydrodynamic volume of molecules. This compound is utilized to regulate the partition coefficient of permeating solutes and maintain osmotic balance, and is commonly applied in gel filtration chromatography for the purification and analysis of biomacromolecules like proteins and nucleic acids. Additionally, Cross-linked dextran G 200 serves as a gel filtration medium (particle size range: 40-120 μm; spherical protein separation range: 5k-600k Da).

Concanavalin A (agarose) consists of Concanavalin A coupled with agarose. This tetrameric metalloprotein lectin, derived from the jack bean Canavalia ensiformis, can bind to molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl, and related spatial residues, facilitating the purification of glycoproteins, polysaccharides, and glycolipids. Additionally, Concanavalin A (agarose) is utilized in various applications, such as the purification of enzyme-antibody conjugates, IgM purification, and membrane vesicle isolation.

Ethylenediaminetetraacetic acid (EDTA) sodium hydrate is a metal chelator that binds with divalent and trivalent metal cations, including calcium. It exhibits antibacterial, anti-inflammatory, antioxidant, anti-hypercalcemic, and anticoagulant properties. EDTA sodium hydrate reduces protein oxidation damage catalyzed by metal ions and maintains a reducing environment during protein purification, making it a common choice for protein purification and storage. Additionally, it can alleviate liver fibrosis and is used in research related to coronary artery disease and neurological disorders.

DBCO-Tetraacetyl mannosamine is an organic compound often employed in biological research for chemical alteration and modification. It is used to modify glycoproteins, cell surface molecules, and other biomolecules, with widespread applications in biolabeling and purification techniques. Additionally, this compound serves as a drug carrier in certain controlled-release drug delivery systems. DBCO-Tetraacetyl mannosamine functions as a click chemistry reagent, containing a DBCO group capable of undergoing strain-promoted alkyne-azide cycloaddition (SPAAC) with azide-containing molecules.

Tetrabutylammonium bromide is an organic ammonium salt compound frequently used in catalytic reactions and purification processes. It demonstrates notable catalytic effects in certain organic synthesis reactions and can serve as a surfactant, stabilizer, and antibacterial agent. Additionally, in some laboratory studies, it is utilized as an ion exchanger and solvent extractor.

Sterile 10 mM Histidine, pH 5.5 buffer is a versatile solution with significant applications in protein purification, vaccine preparation, drug development, cell culture, and electrophoresis. Histidine, an amino acid, provides excellent buffering capacity. Its sterility and specific pH conditions make it ideal for experiments requiring precise environmental control. Histidine in this buffer can derive photosensitizers, which increase significantly upon light exposure. These histidine-derived photosensitizers act as primary mediators in the photooxidation of tryptophan.

DEAE-Dextran (MW 500000) is a complex carbohydrate polymer composed of glucose molecules linked by glycosidic bonds and modified with diethylaminoethyl (DEAE) groups. As a cationic molecule, DEAE-Dextran functions as a transfection agent capable of delivering nucleic acids, such as DNA or RNA, into cells. Its positive charge interacts with negatively charged cell membranes to facilitate nucleic acid uptake. Additionally, DEAE-Dextran serves as an ion exchange chromatography resin, enabling the separation and purification of biomolecules based on their charge.