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Sterile 10 mM Histidine buffer (pH 5.5 )

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Catalog No. TYD-01475
Alias Sterile 10 mM Histidine, pH 5.5 buffer

Sterile 10 mM Histidine buffer (pH 5.5 ) is a widely used biochemical buffer applied in protein purification, vaccine manufacturing, drug development, cell culture, and electrophoretic techniques; Sterile 10 mM Histidine (pH 5.5 buffer) possess strong buffering capacity of histidine, whose imidazole side chain maintains stable acidity, making it ideal for experiments requiring tightly controlled physicochemical conditions; additionally, histidine within this buffer can generate photoreactive derivatives that accumulate dramatically under illumination and serve as primary mediators of tryptophan photo-oxidation which is a key consideration in protein formulation and stability research.

Sterile 10 mM Histidine buffer (pH 5.5 )

Sterile 10 mM Histidine buffer (pH 5.5 )

Copy Product Info
😃Good
Catalog No. TYD-01475Alias Sterile 10 mM Histidine, pH 5.5 buffer
Sterile 10 mM Histidine buffer (pH 5.5 ) is a widely used biochemical buffer applied in protein purification, vaccine manufacturing, drug development, cell culture, and electrophoretic techniques; Sterile 10 mM Histidine (pH 5.5 buffer) possess strong buffering capacity of histidine, whose imidazole side chain maintains stable acidity, making it ideal for experiments requiring tightly controlled physicochemical conditions; additionally, histidine within this buffer can generate photoreactive derivatives that accumulate dramatically under illumination and serve as primary mediators of tryptophan photo-oxidation which is a key consideration in protein formulation and stability research.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
10 mL$37-In Stock
25 mL$74-In Stock
50 mLInquiry-In Stock
100 mLInquiry-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Sterile 10 mM Histidine buffer (pH 5.5 ) is a widely used biochemical buffer applied in protein purification, vaccine manufacturing, drug development, cell culture, and electrophoretic techniques; Sterile 10 mM Histidine (pH 5.5 buffer) possess strong buffering capacity of histidine, whose imidazole side chain maintains stable acidity, making it ideal for experiments requiring tightly controlled physicochemical conditions; additionally, histidine within this buffer can generate photoreactive derivatives that accumulate dramatically under illumination and serve as primary mediators of tryptophan photo-oxidation which is a key consideration in protein formulation and stability research.
SynonymsSterile 10 mM Histidine, pH 5.5 buffer
Chemical Properties
Storage & Solubility Information
Storage-20°C for 20 months | 04

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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