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Sterile 10 mM Histidine buffer (pH 5.5 ) is a widely used biochemical buffer applied in protein purification, vaccine manufacturing, drug development, cell culture, and electrophoretic techniques; Sterile 10 mM Histidine (pH 5.5 buffer) possess strong buffering capacity of histidine, whose imidazole side chain maintains stable acidity, making it ideal for experiments requiring tightly controlled physicochemical conditions; additionally, histidine within this buffer can generate photoreactive derivatives that accumulate dramatically under illumination and serve as primary mediators of tryptophan photo-oxidation which is a key consideration in protein formulation and stability research.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 10 mL | $37 | - | In Stock | |
| 25 mL | $74 | - | In Stock | |
| 50 mL | Inquiry | - | In Stock | |
| 100 mL | Inquiry | - | In Stock |
| Description | Sterile 10 mM Histidine buffer (pH 5.5 ) is a widely used biochemical buffer applied in protein purification, vaccine manufacturing, drug development, cell culture, and electrophoretic techniques; Sterile 10 mM Histidine (pH 5.5 buffer) possess strong buffering capacity of histidine, whose imidazole side chain maintains stable acidity, making it ideal for experiments requiring tightly controlled physicochemical conditions; additionally, histidine within this buffer can generate photoreactive derivatives that accumulate dramatically under illumination and serve as primary mediators of tryptophan photo-oxidation which is a key consideration in protein formulation and stability research. |
| Synonyms | Sterile 10 mM Histidine, pH 5.5 buffer |
| Storage | -20°C for 20 months | 04 |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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