Sterile 10 mM Histidine buffer (pH 5.5 ) is a widely used biochemical buffer applied in protein purification, vaccine manufacturing, drug development, cell culture, and electrophoretic techniques; Sterile 10 mM Histidine (pH 5.5 buffer) possess strong buffering capacity of histidine, whose imidazole side chain maintains stable acidity, making it ideal for experiments requiring tightly controlled physicochemical conditions; additionally, histidine within this buffer can generate photoreactive derivatives that accumulate dramatically under illumination and serve as primary mediators of tryptophan photo-oxidation which is a key consideration in protein formulation and stability research.