p 58

BDP 581 591 NHS ester, a borondipyrromethene dye (Ex=585 nm, Em=594 nm), exhibits a relatively long fluorescence lifetime and significant two-photon excitation cross-section. This compound is applicable in fluorescence polarization analysis, interacts with reactive oxygen species (ROS) to modify fluorescence, and, as an NHS ester derivative, binds to the primary and secondary amine groups of proteins, peptides, and various molecules. Storage instructions highlight the importance of protecting it from light.

BDP 581 591 alkyne, a moderately hydrophobic borondipyrromethene fluorophore, is designed for specialized applications requiring specific fluorescent properties.

BDP 581 591 amine hydrochloride, a BODIPY dye linker, is a universal, photostable fluorophore. The amine group addition enables the compound to react with carboxylic acids, activated NHS esters, and other carbonyl groups [1].

BDP 581 591 azide, an azide derivative of the universal, photostable fluorophore BDP 581 591, can be used for conjugation with small molecules and biomolecules to construct tracers for fluorescence polarization assays and microscopy probes [1].

BDP 581 591 carboxylic acid, a highly photostable fluorescent dye (Ex=585 nm, Em=594 nm), possesses a free carboxylic acid group. This group can undergo catalysis by agents (such as EDC or HATU) to form stable amide bonds with primary amines. The compound is also utilized for ROS detection.

BDP 581 591 maleimide, a linker of the BDP 581 591 dye, has a long fluorescence lifetime and is used for fluorescence polarization assays. The maleimide group reacts with thiol groups to form thioester bonds between pH 6.5 and 7.5, enabling the labeling of sulfhydryl groups of proteins and peptides.