Anti-EIF3L Antibody (2P583) is a Mouse antibody targeting EIF3L. Anti-EIF3L Antibody (2P583) can be used in WB,ICC/IF.

IgG2a, Kappa

2P583

Human (predicted:Mouse)

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:10000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate:1: 293, 2: Raw264.7, 3: 3T3, 4: HepG2
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 67 kDa
Observed MW: 55-70 kDa
2. Cell line: HEK-293
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1: 200
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-05505

WB: 1:2000-10000; ICC/IF: 1:50-200

Monoclonal

EIF3L

Eukaryotic translation initiation factor 3 subunit L

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.