Anti-Dicer Antibody (7P583) is a Mouse antibody targeting Dicer. Anti-Dicer Antibody (7P583) can be used in WB,ICC/IF.

IgG2a

7P583

Human

1. Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1: 250
Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-05144
2. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1: 2000
Primary Ab incubation condition: 2 hours at room temperature
Lysate: 1: HepG2
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 220 kDa
Observed MW: 250 kDa

WB: 1:500-2000; ICC/IF: 1:50-250

Monoclonal

DICER1

Endoribonuclease Dicer

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.