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CY5-SE triethylamine salt

🥰Excellent
Catalog No. T8836Cas No. 1497420-70-8
Alias Fluorolink Cy5 triethanolamine salt, Cy5-SE (triethylamine salt)

CY5-SE triethylamine salt (Fluorolink Cy5 triethanolamine salt) is a hydrophilic amine-reactive fluorescent probe. It displays excitation/emission maxima of 646/662 nm, respectively. Cy5-SE-conjugated ligands have been used in the characterization of hematopoietic tumor microenvironments.

CY5-SE triethylamine salt

CY5-SE triethylamine salt

🥰Excellent
Purity: 98.07%
Catalog No. T8836Alias Fluorolink Cy5 triethanolamine salt, Cy5-SE (triethylamine salt)Cas No. 1497420-70-8
CY5-SE triethylamine salt (Fluorolink Cy5 triethanolamine salt) is a hydrophilic amine-reactive fluorescent probe. It displays excitation/emission maxima of 646/662 nm, respectively. Cy5-SE-conjugated ligands have been used in the characterization of hematopoietic tumor microenvironments.
Pack SizePriceAvailabilityQuantity
1 mg$41In Stock
5 mg$97In Stock
10 mg$147In Stock
25 mg$296In Stock
50 mgPreferentialIn Stock
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Purity:98.07%
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Product Introduction

Bioactivity
Description
CY5-SE triethylamine salt (Fluorolink Cy5 triethanolamine salt) is a hydrophilic amine-reactive fluorescent probe. It displays excitation/emission maxima of 646/662 nm, respectively. Cy5-SE-conjugated ligands have been used in the characterization of hematopoietic tumor microenvironments.
Cell Research
Instructions
I. Preparation of reagents
1. Preparation of mother solution: Use DMSO (or other appropriate solvents), the concentration can be adjusted according to experimental requirements, generally 1 mM.
2. Preparation of working solution: If it is needed for labeling, it can be diluted with PBS or other suitable buffer as needed. The working concentration is generally between 10–100 µM, and the specific concentration is adjusted according to the experimental design.
II. Operation steps
1. Labeling process:
1) Labeling proteins, peptides or oligonucleotides: Mix CY5-SE with target molecules (such as proteins, peptides, oligonucleotides, etc.). At room temperature, the reaction generally takes 30 minutes to 1 hour. The reaction conditions can be carried out in a buffer with a pH value of 7-8.
2) Reaction conditions: CY5-SE reacts with the amino group of the target molecule (such as the amino group of amino acids, the amino group of peptide chains), so the target molecule should have an amino group available for reaction.
3) Removal of unreacted dye: After the reaction is completed, dialysis, gel filtration or ultrafiltration can be used to remove unbound dye. This ensures the purity of the labeling and reduces background fluorescence.
2. Fluorescence detection:
1) Fluorescence microscope or flow cytometer: The labeled samples can be detected by fluorescence microscope or flow cytometer, with an excitation wavelength of 649 nm and an emission wavelength of 670 nm.
2) Fluorescence intensity determination: The intensity of the fluorescence signal is proportional to the number of labeled molecules. By detecting the signal intensity, the target molecules can be quantitatively analyzed.
3. Data analysis:
1) Fluorescence quantitative analysis: According to the change in fluorescence intensity, the concentration of the target molecule can be calculated. The fluorescence intensity can be compared with the standard curve for further quantitative analysis.
2) Control group: The difference in fluorescence intensity between the control group and the experimental group helps to evaluate the effect of different treatment conditions on the labeling effect of the target molecule in the experiment.
AliasFluorolink Cy5 triethanolamine salt, Cy5-SE (triethylamine salt)
Chemical Properties
Molecular Weight855.07
FormulaC43H58N4O10S2
Cas No.1497420-70-8
SmilesCCN(CC)CC.CC[N+]1=C(\C=C\C=C\C=C2/N(CCCCCC(=O)ON3C(=O)CCC3=O)c3ccc(cc3C2(C)C)S(O)(=O)=O)C(C)(C)c2cc(ccc12)S([O-])(=O)=O
Relative Density.no data available
Storage & Solubility Information
Storagekeep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 10 mM, Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.1695 mL5.8475 mL11.6949 mL58.4747 mL
5 mM0.2339 mL1.1695 mL2.3390 mL11.6949 mL
10 mM0.1169 mL0.5847 mL1.1695 mL5.8475 mL

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