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Cyanine5 NHS ester chloride

Name: Cyanine5 NHS ester chloride
Brand: TargetMol
SKU: T38432
Price: 59 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T38432Cas No. 1032678-42-4

Cyanine5 NHS ester chloride is a reactive dye with red fluorescence that requires a small amount of organic cosolvent (such as DMF or DMSO) to label amino groups in peptides, proteins, and oligonucleotides.

Cyanine5 NHS ester chloride

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Purity: 99.87%

Catalog No. T38432Cas No. 1032678-42-4

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$59	In Stock	In Stock
5 mg	$143	In Stock	In Stock
10 mg	$222	In Stock	In Stock
25 mg	$365	In Stock	In Stock
50 mg	$513	In Stock	In Stock
100 mg	$718	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.87%

Appearance:Solid

Color:Yellow

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	Cyanine5 NHS ester chloride is a reactive dye with red fluorescence that requires a small amount of organic cosolvent (such as DMF or DMSO) to label amino groups in peptides, proteins, and oligonucleotides.
In vitro	Anthocyanin-5 NHS ester amine reactive red fluorescent dyes are used to react with free amino groups on cysteine amino acids, which are present in terminal peptides for end-wrapped polybeta-amino ester (pBAEs) skeletons [1] .
Cell Research	Cyanine5 NHS ester chloride fluorescent labeling experiment Operation steps: a. Weigh Cyanine5 NHS ester chloride to prepare a 2 mg/mL methanol solution, take 300 μL and add the substrate to be labeled; b. Gradually add 10 mL of deionized water under high-speed stirring, and react at room temperature in the dark for 2 h; c. After centrifugation and washing, the Cy5-labeled fluorescent material is obtained and stored at 4°C for later use. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

Chemical Properties

Molecular Weight	616.19
Formula	C₃₆H₄₂ClN₃O₄
Cas No.	1032678-42-4
Smiles	[Cl-].CN1\C(=C/C=C/C=C/C2=[N+](CCCCCCCC(=O)ON3C(=O)CCC3=O)c3ccccc3C2(C)C)C(C)(C)c2ccccc12
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 225 mg/mL (365.15 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.6229 mL	8.1144 mL	16.2288 mL	81.1438 mL
5 mM	0.3246 mL	1.6229 mL	3.2458 mL	16.2288 mL
10 mM	0.1623 mL	0.8114 mL	1.6229 mL	8.1144 mL
20 mM	0.0811 mL	0.4057 mL	0.8114 mL	4.0572 mL
50 mM	0.0325 mL	0.1623 mL	0.3246 mL	1.6229 mL
100 mM	0.0162 mL	0.0811 mL	0.1623 mL	0.8114 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Cyanine-5 NHS ester chlorideCyanine5 NHS ester chloride