Lucifer Yellow CH dipotassium is a water-soluble fluorescent tracer that labels endocytosis vesicles in cells.Lucifer Yellow CH dipotassium excites and emits at a maximum wavelength of 430 nm and 540 nm, respectively.
Nile Red (Nile Blue A oxazone) is a strongly fluorescent stain used for the detection of intracellular lipid droplets in the presence of a hydrophobic environment.
Cyanine5 NHS ester chloride is a reactive dye with red fluorescence that requires a small amount of organic cosolvent (such as DMF or DMSO) to label amino groups in peptides, proteins, and oligonucleotides.
Hydroxystilbamidine ((E)-hydroxystilbamidine), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases.
Sulfo-Cy5.5 is a near-infrared fluorescent dye (Ex=673 nm, Em=707 nm) that can be used to label biomolecules such as peptides, proteins, and oligonucleotides [2].
BODIPY 630/650X is a fluorescently labeled conjugate of the adenosine receptor ligand N-ethylcarboxamido-adenosine (NECA), which exhibits distinct excitation and emission maxima at approximately 630 nanometers and 650 nanometers, respectively, making it a valuable tool for fluorescence-based assays and imaging studies targeting adenosine receptor activity and distribution.
DiSC3(5) is a lipophilic cationic fluorescent probe sensitive to changes in membrane potential. 3,3'-Dipropylthiadicarbocyanine iodide accumulates in hyperpolarized cell membranes, leading to fluorescence quenching; when the membranes are depolarized, the dye is released from the cells and the fluorescence intensity is enhanced, which is used for real-time monitoring of mitochondrial membrane potential changes and membrane hyperpolarization. The maximum excitation emission wavelength was 622 670 nm.
DDAO is a promising near-infrared (NIR) red fluorescent probe with a long emission wavelength (λem = 656 nm) and a tunable excitation wavelength (600-650 nm). It can be designed for detecting the activities of various enzymes such as β-galactosidase, sulfatase, protein phosphatase 2A, carboxylesterase 2, human albumin, and esterases.
CY5-SE triethylamine salt (Fluorolink Cy5 triethanolamine salt) is a hydrophilic amine-reactive fluorescent probe. It displays excitation/emission maxima of 646/662 nm, respectively. Cy5-SE-conjugated ligands have been used in the characterization of hematopoietic tumor microenvironments.
Cyanine5.5 amine is an amino-modified Cy5.5 near-infrared (NIR) fluorescent dye with excitation/emission wavelengths of 648/710 nm. It offers the advantage of low fluorescence background and is suitable for group conjugation and in vivo imaging.
Cy5-DBCO is a near-infrared, anthocyanine fluorescent dye Cy5 (Cyanine 5) derivative with an excitation wavelength of 650 nm and an emission wavelength of 670 nm.DBCO-Sulfo-Cy5 contains a DBCO moiety, which can undergo SPAAC click chemistry with molecules that contain Azide moieties.
Alexa fluor 647 maleimide is a water-soluble fluorescent dye that maintains stability under a broad range of physiological conditions. Alexa fluor 647 maleimide facilitates the covalent attachment of fluorescent groups to proteins through selective reaction with thiol groups, thereby enabling precise labeling. This labeling method offers both high sensitivity and specificity, allowing detection of proteins at very low concentrations. Alexa fluor 647 maleimide is widely applied in the study of protein expression and localization, and it can also be conjugated to antibodies for labeling cell surface antigens or specific proteins within tissues [Ex/Em(nm)=651/671].
Cyanine5 amine, hydrochloride can undergo a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction with terminal alkynes and can also react with carboxyl groups to label polysaccharides. It has excitation emission wavelengths of 646 662 nm.
Trx-red (NBL-SS perchlorate) is a red-emitting fluorescent probe derived from the Nile blue fluorophore, specifically designed for imaging thioredoxin (Trx) in live cells and in vivo, with excitation and emission wavelengths of 615 nm and 661 nm, respectively.
sCy5DL-amide is a fluorescent D-amino acid designed for peptidoglycan labeling across a broad spectrum of bacteria, encompassing gram-positive, gram-negative, and mycobacteria strains. It demonstrates exceptional incorporation into the Gram-positive B.subtilis. This compound enables the imaging of labeled bacteria using single molecule localization microscopy (SMLM), also known as PALM or STORM, with excitation emission maxima at 646 666 nm.
sCy5DA, a fluorescent D-amino acid, is designed for annotating peptidoglycans in live bacteria, facilitating the imaging of a broad spectrum of bacterial types, including gram-positive, gram-negative, and mycobacteria, with notably efficient incorporation into gram-negative C. crescentus. This compound allows the bacteria it labels to be visualized through single molecule localization microscopy (SMLM; known as PALM or STORM), with excitation emission maxima reported at 646 665 nm.