Nile Red (Nile Blue A oxazone) is a strongly fluorescent stain used for the detection of intracellular lipid droplets in the presence of a hydrophobic environment.
DDAO is a promising near-infrared (NIR) red fluorescent probe with a long emission wavelength (λem = 656 nm) and a tunable excitation wavelength (600-650 nm). It can be designed for detecting the activities of various enzymes such as β-galactosidase, sulfatase, protein phosphatase 2A, carboxylesterase 2, human albumin, and esterases.
Sulfo-Cy5.5 is a near-infrared fluorescent dye (Ex=673 nm, Em=707 nm) that can be used to label biomolecules such as peptides, proteins, and oligonucleotides [2].
CY5-SE triethylamine salt (Fluorolink Cy5 triethanolamine salt) is a hydrophilic amine-reactive fluorescent probe. It displays excitation emission maxima of 646 662 nm, respectively. Cy5-SE-conjugated ligands have been used in the characterization of hematopoietic tumor microenvironments.
Rf470DL, a rotor-fluorogenic D-amino acid (RfDAA), serves as a bacterial labeling agent, exhibiting an excitation wavelength (Ex) of 470 nm and an emission wavelength (Em) of 640 nm [1].
NIAD-4 is a fluorescent probe that crosses the blood-brain barrier to bind with high affinity to amyloid-β (Aβ) plaques (Ki = 10 nM). It displays excitation emission spectra of 475 625 nm, respectively, with the far-red emission occurring upon binding to Aβ.
DIBA-Cy5, a fluorescent DIBA antagonist, consists of DIBA-alkyne and Cyanine5 fluorophores (Cy5) linked by a polyethylene glycol (PEG) chain. It functions as a fluorescent ligand for probe attachment via click chemistry, exhibiting high binding affinity to type-2 mAChR (M2R) with a dissociation constant (Kd) of 1.80 nM. Notably, DIBA-Cy5 effectively stains M2R receptors in the sinoatrial node of mouse hearts [1].
TFAX 594,SE is a red fluorescent dye that maintains its fluorescence properties across a pH range of 4 to 10. It produces highly intense and photostable conjugates when bound to proteins or antibodies, such as [goat anti-mouse IgG].
meso-CF3-BODIPY 2, also known as CF3-substituted Tetramethyl BODIPY, is a fluorescent dye that has an absorption wavelength (λ abs ) of 553 nm and an emission wavelength (λ em ) of 622 nm. This compound is commonly utilized as a labeling reagent and in photodynamic therapy.
Cyanine 5 Tyramide (Tyramide-Cy5), a red fluorescent dye, acts as a reporter fluorescent substrate in horseradish peroxidase (HRP)-catalyzed deposition, a signal amplification method used in immunoassays and in situ hybridization of nucleic acids.
Cy5-DBCO (DBCO-Sulfo-Cy5) is a near-infrared (NIR) red fluorescent dye (λabs: 646 nm, λem: 670 nm), not suitable for staining intracellular components of permeabilized cells due to potential high background.