Nile Red (Nile Blue A oxazone) is a strongly fluorescent stain used for the detection of intracellular lipid droplets in the presence of a hydrophobic environment.
DDAO is a promising near-infrared (NIR) red fluorescent probe with a long emission wavelength (λem = 656 nm) and a tunable excitation wavelength (600-650 nm). It can be designed for detecting the activities of various enzymes such as β-galactosidase, sulfatase, protein phosphatase 2A, carboxylesterase 2, human albumin, and esterases.
Sulfo-Cy5.5 is a near-infrared fluorescent dye (Ex=673 nm, Em=707 nm) that can be used to label biomolecules such as peptides, proteins, and oligonucleotides [2].
CY5-SE triethylamine salt (Fluorolink Cy5 triethanolamine salt) is a hydrophilic amine-reactive fluorescent probe. It displays excitation emission maxima of 646 662 nm, respectively. Cy5-SE-conjugated ligands have been used in the characterization of hematopoietic tumor microenvironments.
Rf470DL, a rotor-fluorogenic D-amino acid (RfDAA), serves as a bacterial labeling agent, exhibiting an excitation wavelength (Ex) of 470 nm and an emission wavelength (Em) of 640 nm [1].
NIAD-4 is a fluorescent probe that crosses the blood-brain barrier to bind with high affinity to amyloid-β (Aβ) plaques (Ki = 10 nM). It displays excitation emission spectra of 475 625 nm, respectively, with the far-red emission occurring upon binding to Aβ.
Cyanine5 amine, hydrochloride can undergo a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction with terminal alkynes and can also react with carboxyl groups to label polysaccharides. It has excitation emission wavelengths of 646 662 nm.
DIBA-Cy5, a fluorescent DIBA antagonist, consists of DIBA-alkyne and Cyanine5 fluorophores (Cy5) linked by a polyethylene glycol (PEG) chain. It functions as a fluorescent ligand for probe attachment via click chemistry, exhibiting high binding affinity to type-2 mAChR (M2R) with a dissociation constant (Kd) of 1.80 nM. Notably, DIBA-Cy5 effectively stains M2R receptors in the sinoatrial node of mouse hearts [1].
Cyanine5-C6-DBCO is a low-toxicity azide-reactive probe (near-infrared fluorescent dye) used for imaging azide-labeled biomolecules via a copper-free click reaction. Its excitation emission wavelengths are 647 668 nm.
CY5-DBC0 is utilized in cellular imaging to label cell membranes, nuclei, or other organelles, thus revealing cell morphology, structure, and function. In tissue imaging, it helps observe changes in the structure and function of deep tissues like blood vessels. Additionally, CY5-DBC0 is applied in fields such as protein labeling, drug delivery, and biosensing, providing tools and methodologies for biomedical research. The excitation emission (Ex Em) wavelengths are 640 663 nm.
TFAX 594,SE is a red fluorescent dye that maintains its fluorescence properties across a pH range of 4 to 10. It produces highly intense and photostable conjugates when bound to proteins or antibodies, such as [goat anti-mouse IgG].
AF647 C2 maleimide is a water-soluble fluorescent dye that remains stable under a range of physiological conditions. It facilitates the attachment of fluorescent groups to proteins by reacting with thiol groups on target proteins. This labeling method offers high sensitivity and specificity, enabling the detection of proteins at very low concentrations, making it valuable for studying protein expression and localization. Additionally, it can bind to antibodies for labeling cell surface antigens or specific proteins within tissues. [Ex Em(nm)=651 671].
meso-CF3-BODIPY 2, also known as CF3-substituted Tetramethyl BODIPY, is a fluorescent dye that has an absorption wavelength (λ abs ) of 553 nm and an emission wavelength (λ em ) of 622 nm. This compound is commonly utilized as a labeling reagent and in photodynamic therapy.
Cyanine 5 Tyramide (Tyramide-Cy5), a red fluorescent dye, acts as a reporter fluorescent substrate in horseradish peroxidase (HRP)-catalyzed deposition, a signal amplification method used in immunoassays and in situ hybridization of nucleic acids.
Cy5-DBCO (DBCO-Sulfo-Cy5) is a near-infrared (NIR) red fluorescent dye (λabs: 646 nm, λem: 670 nm), not suitable for staining intracellular components of permeabilized cells due to potential high background.