CY7 (Sulfo-Cyanine7) is a fluorescence labeling agent (Ex=750 nm, Em=773 nm). Cy7 is used for labeling oligonucleotides, antibodies, peptides, and proteins.
CY7-SE Triethylamine is a fluorescence labeling agent (Ex=700-770 nm,Em=790 nm) used for labeling proteins, peptides, antibodies, and oligonucleotides.
5(6)-FAM SE (5(6)-Carboxyfluorescein N-hydroxysuccinimide ester) is an amine-reactive green fluorescent dye. 5(6)-FAM SE is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.
Sulfo-Cy5.5 is a near-infrared fluorescent dye (Ex=673 nm, Em=707 nm) that can be used to label biomolecules such as peptides, proteins, and oligonucleotides [2].
Dansyl chloride (DNSC-l) reacts with primary amino groups in both aliphatic and aromatic amines to produce stable blue- or blue-green-fluorescent sulfonamide adducts. It is widely used to modify amino acids, specifically, protein sequencing and amino acid analysis.
Sulforhodamine B sodium salt (Acid Red 52) is a fluorescent dye. It can be used from laser-induced fluorescence to the quantification of cellular proteins of cultured cells.
CY5-SE triethylamine salt (Fluorolink Cy5 triethanolamine salt) is a hydrophilic amine-reactive fluorescent probe. It displays excitation/emission maxima of 646/662 nm, respectively. Cy5-SE-conjugated ligands have been used in the characterization of hematopoietic tumor microenvironments.
5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.
CY3-YNE (Sulfo-Cyanine3-alkyne) is a dye labeled with a biomarker group alkyne, which can be used to label proteins, antibodies, peptides, nucleic acid molecules, and nanoparticles (Ex/Em: 554/566 nm).
Dabsyl chloride (DABS-Cl) is an amine derivatizing agent and able to give rise to stable products that can be easily monitored spectrophotometrically at 460 nm.
2-(4-Hydroxyphenylazo)benzoicacid is a spectrophotometric probe that shows changes in absorption spectra upon binding to proteins and binds to bovine serum proteins.
5-FAM SE is a single isomer widely employed in the preparation of various small fluorescent molecules. It is a highly popular green fluorescent reagent used for labeling peptides, proteins, and nucleotides [5-FAM SE].
6-CR110 [6-Carboxyrhodamine 110] *Single isomer* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.
AF647 C2 maleimide is a water-soluble fluorescent dye that remains stable under a range of physiological conditions. It facilitates the attachment of fluorescent groups to proteins by reacting with thiol groups on target proteins. This labeling method offers high sensitivity and specificity, enabling the detection of proteins at very low concentrations, making it valuable for studying protein expression and localization. Additionally, it can bind to antibodies for labeling cell surface antigens or specific proteins within tissues. [Ex/Em(nm)=651/671].
bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation.[1] Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings.[2] bis-ANS has an excitation maxima of 390 nm.[3] It has an emission maximum of 523 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FAPB2) it has emission maxima of 484-496 nm. bis-ANS has been used to label mechanically damaged neurons in acute brain slices.4 It also potently inhibits microtubule assembly.[5],[6]