Beryllon II is a widely used chromogenic reagent for determining proteins and various elements, including Mg, Mo, and Co.

Beryllon II detects RLS spectra in serum
I. Solution preparation
1. Mother solution preparation: Beryllon II is directly dissolved in water to prepare a 1000 μmol/L stock standard solution.
2. Working solution preparation: Dilute with water to a 250 μmol/L working solution when used.
II. Operation steps
1. Serum sample preparation: Serum samples are diluted 400 times with deionized water to prepare standard stock solutions and stored at 0–4°C.
2. Walpole buffer (1.0 mL), appropriate volumes of working standard Beryllon II and serum samples are mixed separately, then diluted with water to 10.0 mL and stirred thoroughly;
3. Use a spectrophotometer to synchronously scan 300–550 nm to detect RLS spectra.