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Beryllon II is a widely used chromogenic reagent for determining proteins and various elements, including Mg, Mo, and Co.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 200 mg | $29 | Inquiry | Inquiry | |
| 1 mL x 10 mM (in DMSO) | $29 | In Stock | In Stock |
| Description | Beryllon II is a widely used chromogenic reagent for determining proteins and various elements, including Mg, Mo, and Co. |
| In vitro | Beryllon II detects RLS spectra in serum I. Solution preparation 1. Mother solution preparation: Beryllon II is directly dissolved in water to prepare a 1000 μmol/L stock standard solution. 2. Working solution preparation: Dilute with water to a 250 μmol/L working solution when used. II. Operation steps 1. Serum sample preparation: Serum samples are diluted 400 times with deionized water to prepare standard stock solutions and stored at 0–4°C. 2. Walpole buffer (1.0 mL), appropriate volumes of working standard Beryllon II and serum samples are mixed separately, then diluted with water to 10.0 mL and stirred thoroughly; 3. Use a spectrophotometer to synchronously scan 300–550 nm to detect RLS spectra. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Molecular Weight | 738.52 |
| Formula | C20H10N2Na4O15S4 |
| Cas No. | 51550-25-5 |
| Smiles | OC1=C(C(/N=N/C2=C(O)C(C(O)=CC(S(=O)(O[Na])=O)=C3)=C3C=C2S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C4)C4=CC(S(=O)(O[Na])=O)=C1 |
| Relative Density. | no data available |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 60 mg/mL (81.24 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween-80+45% Saline: 1 mg/mL (1.35 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | ||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||
DMSO
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Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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