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Beryllon II

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Catalog No. T18925Cas No. 51550-25-5

Beryllon II is a widely used chromogenic reagent for determining proteins and various elements, including Mg, Mo, and Co.

Beryllon II

Beryllon II

😃Good
Purity: 99.84%
Catalog No. T18925Cas No. 51550-25-5
Beryllon II is a widely used chromogenic reagent for determining proteins and various elements, including Mg, Mo, and Co.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
200 mg$29InquiryInquiry
1 mL x 10 mM (in DMSO)$29In StockIn Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Batch Information

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Purity:99.84%
Appearance:Solid
Color:Black
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Product Introduction

Bioactivity
Description
Beryllon II is a widely used chromogenic reagent for determining proteins and various elements, including Mg, Mo, and Co.
In vitro
Beryllon II detects RLS spectra in serum
I. Solution preparation
1. Mother solution preparation: Beryllon II is directly dissolved in water to prepare a 1000 μmol/L stock standard solution.
2. Working solution preparation: Dilute with water to a 250 μmol/L working solution when used.
II. Operation steps
1. Serum sample preparation: Serum samples are diluted 400 times with deionized water to prepare standard stock solutions and stored at 0–4°C.
2. Walpole buffer (1.0 mL), appropriate volumes of working standard Beryllon II and serum samples are mixed separately, then diluted with water to 10.0 mL and stirred thoroughly;
3. Use a spectrophotometer to synchronously scan 300–550 nm to detect RLS spectra.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Chemical Properties
Molecular Weight738.52
FormulaC20H10N2Na4O15S4
Cas No.51550-25-5
SmilesOC1=C(C(/N=N/C2=C(O)C(C(O)=CC(S(=O)(O[Na])=O)=C3)=C3C=C2S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C4)C4=CC(S(=O)(O[Na])=O)=C1
Relative Density.no data available
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 60 mg/mL (81.24 mM), Sonication is recommended.
In Vivo Formulation
10% DMSO+40% PEG300+5% Tween-80+45% Saline: 1 mg/mL (1.35 mM), Sonication is recommended.
Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.3541 mL6.7703 mL13.5406 mL67.7030 mL
5 mM0.2708 mL1.3541 mL2.7081 mL13.5406 mL
10 mM0.1354 mL0.6770 mL1.3541 mL6.7703 mL
20 mM0.0677 mL0.3385 mL0.6770 mL3.3851 mL
50 mM0.0271 mL0.1354 mL0.2708 mL1.3541 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

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