CY7-SE Triethylamine is a fluorescence labeling agent (Ex=700-770 nm,Em=790 nm) used for labeling proteins, peptides, antibodies, and oligonucleotides.

Instructions for use
I. Solution preparation: Prepare a 10 mM stock solution with DMSO. Mix well by pipetting or vortexing.
I. Protein preparation
1. For the best labeling effect, prepare the protein (antibody) concentration to 2 mg/mL.
2. The pH value of the protein solution is 8.5±0.5. If the pH value is lower than 8.0, 1M sodium bicarbonate should be used for adjustment;
3. If the protein concentration is lower than 2mg/mL, the labeling efficiency will be greatly reduced. For optimal labeling efficiency, the recommended final protein concentration range is 2-10 mg/mL;
4. The protein must be in a buffer that does not contain primary amines (such as Tris or glycine) and ammonium ions, otherwise it will affect the labeling efficiency;
3. Calculation of dye dosage: The amount of CY7-SE Triethylamine required for the reaction depends on the amount of protein to be labeled, and the optimal molar ratio is about 10;
4. Run the coupling reaction
1) Slowly add an appropriate amount of freshly prepared 10 mg/mL CY7-SE Triethylamine to 0.5 mL of protein sample
In the solution, gently shake to mix, then briefly centrifuge and collect the sample at the bottom of the reaction tube. Do not mix to avoid denaturation and inactivation of the protein sample.
2) Place the reaction tube in a dark place and gently incubate at room temperature for 60 minutes. 10-15 minutes, gently reverse the reaction.
5. Purify the conjugate

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

DMSO: 10 mM, Sonication is recommended.