chromatography

AQC (6-Aminoquinolyl-N-hydroxysccinimidyl carbamate) is a reagent used in HPLC for the fluorescence detection of amino acid or protein sequences. It reacts with primary and secondary amino acids to produce fluorescent derivatives, enabling the detection of amino acids at picomolar levels.

N-Hydroxysuccinimide (NHS) is a commonly employed activating ester in peptide chemistry and protein cross-linking, typically utilised to activate carboxyl groups for coupling with amine groups, serving as a key activating agent in bio-conjugate reactions.

Sulfo-Cy3-PEG3-biotin potassium is a biotin-modified derivative of the Cy3 (Cyanine3) dye, featuring a TCO group. This TCO group can undergo a highly specific click chemistry reaction with tetrazine functional groups to form covalent bonds. Consequently, Sulfo-Cy3-PEG3-biotin potassium can be covalently linked to various biomolecules (particularly antibodies and proteins), facilitating their location and dynamic monitoring in biological samples. Additionally, due to its biotin labeling, it is commonly employed in affinity chromatography experiments, such as immunoprecipitation.

5-Phenyl-4E-pentenol is a product of PPHP reduction catalyzed by peroxidases. It can be rapidly separated using solid-phase extraction and quantified through isocratic reverse-phase high-performance liquid chromatography (HPLC) coupled with UV detection. As a fluorescent dye, 5-Phenyl-4E-pentenol is used to determine the kinetic properties of both heme and non-heme peroxidases and to evaluate the capability of oxidative compounds as substrates for peroxidase reduction.

Maackia amurensis Lectin (MAA/MAL II)-Biotinylated is a biotin-modified plant lectin that recognizes specific carbohydrate structures, particularly α-2,3 linked sialic acids. It exhibits high affinity for avidin or streptavidin, facilitating its attachment to solid surfaces or binding with other molecules. This lectin is useful in affinity chromatography for the isolation and purification of proteins or other molecules with specific glycan structures, and it aids in disease biomarker discovery and cancer research.

7-Methoxycoumarin-4-acetic acid (MCA) is a coumarin-derived fluorescent dye for peptide labelling. MCA enables quantitative detection of platelet-activating factor (PAF) via high-performance liquid chromatography fluorescence detection (Ex/Em=330/390 nm).