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Fast Blue B Salt

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Catalog No. T78453 Copy Product Info
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Fast Blue B Salt is a diazo salt dye primarily used in histochemistry and enzymatic assays. When coupled with phenolic compounds such as 1-naphthol, Fast Blue B Salt forms a purple diazo dye, making it widely used in colorimetric assays for acetylcholinesterase. In addition, Fast Blue B Salt is commonly used as a spray-staining agent in thin-layer chromatography for the qualitative and quantitative analysis of aflatoxins, and for staining chromaffin cells in the intestine in histology.
Fast Blue B Salt
Cas No. 14263-94-6
Pack SizePriceUSA StockGlobal StockQuantity
500 mg$29-In Stock
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Appearance:Solid
Color:Green
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Product Introduction

Bioactivity
Description
Fast Blue B Salt is a diazo salt dye primarily used in histochemistry and enzymatic assays. When coupled with phenolic compounds such as 1-naphthol, Fast Blue B Salt forms a purple diazo dye, making it widely used in colorimetric assays for acetylcholinesterase. In addition, Fast Blue B Salt is commonly used as a spray-staining agent in thin-layer chromatography for the qualitative and quantitative analysis of aflatoxins, and for staining chromaffin cells in the intestine in histology.
Chemical Properties
Molecular Weight475.49
FormulaC14H12Cl4N4O2Zn
Cas No.14263-94-6
SmilesN#[N+]C=1C=CC(=CC1OC)C2=CC=C([N+]#N)C(OC)=C2.[Cl-][Zn+2]([Cl-])([Cl-])[Cl-]
Storage & Solubility Information
StorageKeep away from moisture,Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 80 mg/mL (168.25 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.1031 mL10.5155 mL21.0309 mL105.1547 mL
5 mM0.4206 mL2.1031 mL4.2062 mL21.0309 mL
10 mM0.2103 mL1.0515 mL2.1031 mL10.5155 mL
20 mM0.1052 mL0.5258 mL1.0515 mL5.2577 mL
50 mM0.0421 mL0.2103 mL0.4206 mL2.1031 mL
100 mM0.0210 mL0.1052 mL0.2103 mL1.0515 mL
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 µL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
µL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Fast Blue B Salt chemical structure | Fast Blue B Salt formula | Fast Blue B Salt molecular weight