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N-Hydroxysuccinimide

Name: N-Hydroxysuccinimide
Brand: TargetMol
SKU: T87003
Price: 38 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: HOSu, 1-Hydroxy-2,5-pyrrolidinedione) Copy Product Info

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Synonyms: HOSu, 1-Hydroxy-2,5-pyrrolidinedione

Catalog No. T87003 Copy Product Info

Purity: 99.93%

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N-Hydroxysuccinimide (NHS) is a commonly employed activating ester in peptide chemistry and protein cross-linking, typically utilised to activate carboxyl groups for coupling with amine groups, serving as a key activating agent in bio-conjugate reactions.

Cas No. 6066-82-6

Pack Size	Price	USA Stock	Global Stock	Quantity
10 g	$38	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.93%

Appearance:Solid

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	N-Hydroxysuccinimide (NHS) is a commonly employed activating ester in peptide chemistry and protein cross-linking, typically utilised to activate carboxyl groups for coupling with amine groups, serving as a key activating agent in bio-conjugate reactions.
In vitro	In the collagen solution cross-linking experiment, N-Hydroxysuccinimide-activated adipic acid was confirmed by SDS-PAGE analysis to induce the formation of intermolecular cross-links in collagen. Fluorescence spectroscopy Results indicated enhanced microenvironmental polarity of collagen after cross-linking, while atomic force microscopy observations revealed randomly aggregated states of collagen molecules. These changes contribute to improving the self-assembly capability of collagen. [1]
Synonyms	HOSu, 1-Hydroxy-2,5-pyrrolidinedione

Chemical Properties

Molecular Weight	115.09
Formula	C₄H₅NO₃
Cas No.	6066-82-6
Smiles	O=C1N(O)C(=O)CC1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 257.5 mg/mL (2237.38 mM), Sonication is recommended.

H2O: ≥ 80 mg/mL, Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 10 mg/mL (86.89 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	8.6889 mL	43.4443 mL	86.8885 mL	434.4426 mL
5 mM	1.7378 mL	8.6889 mL	17.3777 mL	86.8885 mL
10 mM	0.8689 mL	4.3444 mL	8.6889 mL	43.4443 mL
20 mM	0.4344 mL	2.1722 mL	4.3444 mL	21.7221 mL
50 mM	0.1738 mL	0.8689 mL	1.7378 mL	8.6889 mL
100 mM	0.0869 mL	0.4344 mL	0.8689 mL	4.3444 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: N-Hydroxysuccinimide chemical structure | N-Hydroxysuccinimide in vitro | N-Hydroxysuccinimide formula | N-Hydroxysuccinimide molecular weight