Shopping Cart

Remove All
Your shopping cart is currently empty

N-Hydroxysuccinimide

Name: N-Hydroxysuccinimide
Brand: TargetMol
SKU: T87003
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T87003Cas No. 6066-82-6

Alias HOSu, 1-Hydroxy-2,5-pyrrolidinedione

N-Hydroxysuccinimide (NHS) is a commonly employed activating ester in peptide chemistry and protein cross-linking, typically utilised to activate carboxyl groups for coupling with amine groups, serving as a key activating agent in bio-conjugate reactions.

N-Hydroxysuccinimide

🥰Excellent

Catalog No. T87003Alias HOSu, 1-Hydroxy-2,5-pyrrolidinedioneCas No. 6066-82-6

Pack Size	Price	Availability	Quantity
1 g	$29	In Stock

Add to Cart

Add to Quotation

Bulk & Custom

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Resource Download

COA HNMR

Product Introduction

Bioactivity

Description	N-Hydroxysuccinimide (NHS) is a commonly employed activating ester in peptide chemistry and protein cross-linking, typically utilised to activate carboxyl groups for coupling with amine groups, serving as a key activating agent in bio-conjugate reactions.
In vitro	In the collagen solution cross-linking experiment, N-Hydroxysuccinimide-activated adipic acid was confirmed by SDS-PAGE analysis to induce the formation of intermolecular cross-links in collagen. Fluorescence spectroscopy Results indicated enhanced microenvironmental polarity of collagen after cross-linking, while atomic force microscopy observations revealed randomly aggregated states of collagen molecules. These changes contribute to improving the self-assembly capability of collagen. [1]
Synonyms	HOSu, 1-Hydroxy-2,5-pyrrolidinedione

Chemical Properties

Molecular Weight	115.09
Formula	C₄H₅NO₃
Cas No.	6066-82-6
Smiles	O=C1N(O)C(=O)CC1
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 80 mg/mL (695.11 mM), Sonication is recommended.

H2O: ≥ 80 mg/mL, Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	8.6889 mL	43.4443 mL	86.8885 mL	434.4426 mL
5 mM	1.7378 mL	8.6889 mL	17.3777 mL	86.8885 mL
10 mM	0.8689 mL	4.3444 mL	8.6889 mL	43.4443 mL
20 mM	0.4344 mL	2.1722 mL	4.3444 mL	21.7221 mL
50 mM	0.1738 mL	0.8689 mL	1.7378 mL	8.6889 mL
100 mM	0.0869 mL	0.4344 mL	0.8689 mL	4.3444 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc