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Nuclear dyes can be divided into basic dyes (such as hematoxylin, methyl green), fluorescent dyes, nucleic acid-specific dyes, live cell dyes, and metal complex dyes according to their chemical properties and applications. Basic dyes bind to nucleic acids through charge, while fluorescent dyes specifically label DNA and emit fluorescence, which are suitable for microscopic observation and flow analysis; nucleic acid-specific dyes (such as Feulgen) quantify DNA through chemical reactions, while live cell dyes can penetrate the cell membrane and label live nuclei. These dyes are widely used in histology, cell biology, and molecular pathology research.
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