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NucPE1

Name: NucPE1
Brand: TargetMol
SKU: T19042
Rating: 4.5 (1 reviews)

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Catalog No. T19042Cas No. 1404091-23-1

Alias Nuclear Peroxy Emerald 1

NucPE1, a nuclear-localized fluorescent hydrogen peroxide, is specifically localized to cellular nuclei without appended targeting moieties.

NucPE1

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Catalog No. T19042Alias Nuclear Peroxy Emerald 1Cas No. 1404091-23-1

NucPE1, a nuclear-localized fluorescent hydrogen peroxide, is specifically localized to cellular nuclei without appended targeting moieties.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
5 mg	$2,049	7-10 days	7-10 days
10 mg	Inquiry	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	NucPE1, a nuclear-localized fluorescent hydrogen peroxide, is specifically localized to cellular nuclei without appended targeting moieties.
In vitro	NucPE1 exhibits two primary absorptions in the visible region (λabs=468 nm, ε=27,300 M-1cm-1; λabs=490 nm, ε=26,000 /(M·cm)) and a weak emission (λem=530 nm, Φ=0.117). The reaction of NucPE1 with H2O2 induces a fluorescence increase by converting it to fluorophore NucPE1, which shows a major absorption at 505 nm (ε=19,100 /(M·cm)) and enhanced emission (λem=530 nm, Φ=0.626). NucPE1 selectively accumulates in the nuclei of various mammalian cell lines and in whole model organisms like C. clegans, where it can detect subcellular H2O2 flux changes.
In vivo	NucPE1 imaging reveals a reduction in nuclear H2O2 levels in worms overexpressing sir-2.1 compared to wildtype congeners.
Synonyms	Nuclear Peroxy Emerald 1

Chemical Properties

Molecular Weight	483.36
Formula	C₂₉H₃₀BNO₅
Cas No.	1404091-23-1
Smiles	CCNc1cc2Oc3cc(ccc3C3(OC(=O)c4ccccc34)c2cc1C)B1OC(C)(C)C(C)(C)O1
Relative Density.	1.26 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 25 mg/mL (51.72 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2 mg/mL (4.14 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0689 mL	10.3443 mL	20.6885 mL	103.4426 mL
5 mM	0.4138 mL	2.0689 mL	4.1377 mL	20.6885 mL
10 mM	0.2069 mL	1.0344 mL	2.0689 mL	10.3443 mL
20 mM	0.1034 mL	0.5172 mL	1.0344 mL	5.1721 mL
50 mM	0.0414 mL	0.2069 mL	0.4138 mL	2.0689 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

NucPE-1NucPE1

NucPE1

NucPE1

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Product Introduction

Calculator

In Vivo Formulation Calculator (Clear solution)

Dose Conversion

Tech Support

Keywords