Cytoplasm

Evans blue (C.I. 23860) is an azo dye, inhibits L-glutamate uptake via the membrane-bound excitatory amino acid transporter (EAAT).

7-Hydroxy-4-methylcoumarin-3-acetic acid is a blue fluorophore with pH-dependent and environmentally sensitive fluorescence that is widely used to label peptides, nucleotides, and carbohydrates, and also as a pH indicator.

CellTracker Blue CMAC, a fluorescent dye, features a chloromethyl group that forms a covalent bond with cell proteins, allowing stable attachment and facilitating long-term cell tracking [1].

5-diacetate FAM NHS (5-CFDA SE) is a cell-permeable fluorescent tracer for use in fluorescence microscopy and flow cytometry, Ex=492 nm, Em=517 nm.

BODIPY TR methyl ester serves as a lipophilic GFP counterstain, easily permeating cell membranes to accumulate in endomembranous organelles, while exhibiting minimal localization in plasma membranes. It is a highly effective red fluorescent vital dye (Ex=568 nm, Em=625 nm) that can reveal the location and shape of cell nuclei, the morphology of cells in embryonic tissues, and the boundaries of organ-forming tissues within an entire embryo [1].

8RK59 is a PARK7 inhibitor.

CellTracker CM-DiI (CM-DiI) is a lipophilic fluorochrome designed for tracking lymphocyte migration. It effectively detects labeled cells in histological sections (excitation 553 nm; emission 570 nm) [1] [2].

5-CFDA-AM is a cell-permeable esterase substrate used as an active probe to evaluate enzyme activity and cell membrane integrity. Electroneutral and able to enter cells at lower concentrations than CFDA, it undergoes intracellular hydrolysis by esterases to produce carboxyfluorescein, which possesses an extra negative charge, enhancing cellular retention [1].