kinetic

4-Methylumbelliferyl phosphate (4-MUP) (4-MUP) is used as a fluorogenic substrate of alkaline phosphatases.

Dnp-RPLALWRS TFA is a fluorescent peptide substrate designed specifically for human matrilysin (MMP-7). Upon enzymatic cleavage at the alanine-leucine bond, the release of the Dnp group alleviates fluorescence quenching, enabling real-time quantitative analysis of MMP-7 activity by enhancing tryptophan emission. This compound offers a sensitive and efficient method for kinetic studies and inhibitor screening.

5-Phenyl-4E-pentenol is a product of PPHP reduction catalyzed by peroxidases. It can be rapidly separated using solid-phase extraction and quantified through isocratic reverse-phase high-performance liquid chromatography (HPLC) coupled with UV detection. As a fluorescent dye, 5-Phenyl-4E-pentenol is used to determine the kinetic properties of both heme and non-heme peroxidases and to evaluate the capability of oxidative compounds as substrates for peroxidase reduction.

BODIPY-Cyclopamine is a fluorescent ligand for the Smoothened (SMO) receptor. SMO activation, which is regulated by Patch protein, is linked to tumorigenesis when excessively activated. Using NanoBRET technology, sensitive detection of the resonance energy transfer between SMO and BODIPY-Cyclopamine can be achieved, facilitating high-throughput screening and kinetic analysis. Additionally, studying the binding sites of BODIPY-Cyclopamine on SMO can aid in discovering SMO-targeted drugs.