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Other enzyme substrates

Other enzyme substrate dyes refer to compounds used to detect the activity of non-protease enzymes, including substrates of glycosidases, phosphatases, and oxidoreductases. These substrate dyes generate detectable colorimetric or luminescent signals through enzyme-catalyzed reactions, playing an important role in enzymology research, drug development, and clinical diagnosis.

C12FDG
5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside
T78393138777-25-0In house
C12FDG (5-Dodecanoylaminofluorescein di-beta-D-Galactopyranoside) is used as a lipophilic green fluorescent substrate for detecting beta-galactose activity, a green fluorescent substrate, is a senescence marker and is often used in conjunction with flow cytometry.
  • $163
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TargetMol | Citations Cited
Fluorescein diphosphate tetraammonium
Fluorescein diphosphate Tetraammonium salt, FDP
T20421217305-49-2
Fluorescein diphosphate tetraammonium is a fluorescent dye and a fluorescent phosphatase substrate that is hydrolyzed into highly fluorescent fluorescein after interaction with phosphatases.
  • $112
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X-GAL
BCIG
T190757240-90-6
X-GAL (BCIG) is a widely used chromogenic substrate for β-galactosidase, which cleaves X-GAL to produce an insoluble blue compound.
  • $56
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7-Azido-4-methylcoumarin
7-azido-4-methyl-2H-chromen-2-one;
T3700095633-27-5
7-Azido-4-methylcoumarin (7-azido-4-methyl-2H-chromen-2-one) is a fluorescent photoactive probe used in the study of human phenol sulfotransferase (SULT1A1 or P-PST-1) substrate binding sites through photoaffinity labeling.
  • $31
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Suc-AAPF-pNA
Suc-Ala-Ala-Pro-Phe-pNA
T7843270967-97-4
Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) is a substrate chromogen that releases a yellow 4-nitroaniline upon cleavage and is a substrate for many proteases and prostate-specific antigens (PAs), and can be used to identify novel protein hydrolytic activities in yeast mutants.
  • $40
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2-Chloro-4-nitrophenyl-α-D-glucopyranoside
CNP-α-D-Glucopyaoside
T37835119047-14-2
2-Chloro-4-nitrophenyl-α-D-glucopyranoside (CNP-α-D-Glucopyraoside) is used for measuring α-glucosidase activity.
  • $29
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X-Gluc cyclohexanamine
T78428114162-64-0
X-Gluc cyclohexanamine is a dye used to detect β-glucuronidase (GUS) activity and serves as a marker for gene expression, enabling the detection of target gene expression.
  • $35
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GPDA
GPN, Glycylproline p-nitroanilide tosylate
T1899765096-46-0
GPDA (Glycylproline p-nitroanilide tosylate) is a substrate of X-Prolyl dipeptidyl-aminopeptidase in the enzyme assay.
  • $34
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7-hydroxycoumarinyl-γ-Linolenate
T37855161180-12-7
7-hydroxycoumarinyl-γ-Linolenate is a substrate for cytoplasmic phospholipase A2 (cPLA2), which shows cPLA2α enzymatic activity through hydrolysis of 7-hydroxycoumarinyl-γ-linolenate.
  • $30
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EDANS
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid], EDANS acid [5-((2-Aminoethyl)amino)napht, 1,5-EDANS
TD008150402-56-7
EDANS (EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET).
  • $40
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1-Naphthyl acetate
T19084830-81-9
1-Naphthyl acetate is usually used in a rapid staining method for identification of macrophages. 1-Naphthyl acetate is a potent chromogenic substrate for the detection of erythrocyte acetylcholinesterase (AChE) activity. It has the potential to detect organophosphorus pesticide poisoning.
  • $30
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Boc-Val-Pro-Arg-MCA TFA
T78409L
Boc-Val-Pro-Arg-MCA TFA is a sensitive fluorescent substrate that is a thrombin substrate for the determination of trypsin-like serine protease activity.
  • $89
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4-Methylumbelliferyl heptanoate
4-Methylumbelliferyl enanthate
T8364318319-92-1
4-Methylumbelliferyl heptanoate (4-Methylumbelliferyl enanthate) is a lipase fluorescent substrate that detects cell-mediated cytotoxicity and cell proliferation.
  • $44
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TMB-PS
TMBZ-PS
T7109102062-36-2
TMB-PS (TMBZ-PS) is a chromogenic reagent for the detection of hydrogen peroxide e.g. in the enzymatic analysis of body fluids.
  • $33
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3-Cyano-7-ethoxycoumarin
T7696117620-77-6
3-Cyano-7-ethoxycoumarin is cytochrome P450 (CYP) isoforms CYP1A1 and CYP1A2 .
  • $38
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Z-Gly-Pro-Arg-AMC hydrochloride
T80092201928-42-9
Z-Gly-Pro-Arg-AMC hydrochloride is a synthetic peptide that is often used as a trypsin and histone K fluorescent substrate to measure enzyme activity, which fluoresces when the enzyme is cleaved from the peptide.
  • $56
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5-Phospho-D-ribose 1-diphosphate sodium salt hydrate
T36973
5-Phospho-D-ribose 1-diphosphate sodium salt hydrate (PRPP sodium salt hydrate) is a substrate for o-aminobenzoic acid phosphoribosyltransferase.
  • $185
35 days
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CENTA
T3079280072-86-2
CENTA is a newly synthesized β-lactamase-unstable colorimetric cephalosporin reagent that is readily hydrolyzed by all classes of β-lactamases except Aeromonas hydrophila metalloenzymes.
  • $113
35 days
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Firefly Luciferase mRNA(N1-Me-Pseudo UTP)
FLuc mRNA (N'-Me-Pseudo UTP)
T82397
Firefly Luciferase mRNA (N1-Me-Pseudo UTP) is an mRNA sequence encoding firefly luciferase, which can express luciferase protein in cells after transfection, and is commonly used in gene regulation, cell viability assay, ATP level analysis and in vivo imaging, etc. It can be used as a positive control for mRNA transfection, and yellow-green fluorescence is observed at 560 nm.
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HB-2-30
T2049983031522-14-9
HB-2-30 is a far-red fluorescent transglutaminase 2 (TG2) probe for in vitro and in vivo endocytosis imaging. HB-2-30 complexes with TG2 and α2-macroglobulin for efficient endocytosis via the LRP1 pathway.
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C.I. 37530
T30654135-62-6
C.I. 37530 can be used as a color developer.
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7-10 days
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PF-Alkyne
PF Alkyne
T33957
PF-Alkyne is a fluorophosphonate-based probe which specifically and covalently reacts with the tyrosine-111 residue of the Schistosoma japonicum GST (sjGST) tag. PF-Alkyne rapidly and site-selectively immobilizes sjGST fusion proteins.
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3-6 months
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QTZ
T784292883232-39-9
QTZ, a coelenterazine analog with a 4-quinolinyl substitution at the C8 position of the imidazopyrazinone core [1], serves as a bioluminescence agent for in vivo imaging. This compound features red-shifted emission and produces minimal background interference.
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Suc-Ala-Ala-Phe-AMC
T7840771973-79-0
Suc-Ala-Ala-Phe-AMC, a fluorogenic chymotrypsin substrate hydrolyzable by endopeptidase, has applications in vivo for assaying the acrosome reaction and in vitro for enzyme assays [1] [2] [3].
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