Ex 400-430 nm Purple

Lucifer Yellow CH dipotassium is a water-soluble fluorescent tracer that labels endocytosis vesicles in cells.Lucifer Yellow CH dipotassium excites and emits at a maximum wavelength of 430 nm and 540 nm, respectively.

Laurdan (6-Dodecanoyl-2-dimethylaminonaphthalene) is a fluorescent membrane probe that is spectrally sensitive to the phospholipid phase of the cell membrane to which it binds.

Bromobimane (Monobromobimane) is a non-fluorescent substance, but reacts with thiols to produce a fluorescent product.Bromobimane is used as a probe, often in clinical medicine, to test for sulfide levels in blood.

Coumarin hydrazine is a fluorophore containing an aromatic hydrazine moiety that serves as a fluorescent chemical probe for labeling intracellular protein- and lipid-bound carbonyls, and as a fluorescent sensor in living cells for the detection of carbonylated biomolecules produced following oxidative stress.

2-Aminoacridone is a widely used fluorophore (λexc=428 nm, λem=525 nm).

Benzenepentacarboxylic Acid (Pentacarboxybenzene) is a novel fluorescent probe to detect and scavenge HO-. Benzenepentacarboxylic acid is utilized to prove and detect the generation of HO- in H2O2/TAED and H2O2/TBCC alkali systems.

Lucifer Yellow CH dilithium salt is a fluorescent dye commonly utilized for selective staining and studying the photodynamic disruption of eukaryotic and subcellular structures.

CytoCalcein Violet 450 is a fluorogenic dye used to assess cell viability. It is a cell-permeable dye that, upon entering cells, is cleaved by intracellular esterases. CytoCalcein Violet 450 is retained by living cells and displays excitation emission maxima of 405 450 nm, respectively.

PB succinimidyl ester (Ocean Blue, SE) is a fluorescent compound characterized by an excitation emission wavelength of approximately 405 455 nm.

NBD-X, SE, serving as an acceptor fluorophore, facilitates the generation of environment-sensitive bioconjugates [1].

TFMU-ADPr serves as a versatile substrate for assessing poly(ADP-ribose) glycohydrolase (PARG) activity, enabling direct measurement of total PAR hydrolase activity through the liberation of a fluorophore. Exhibiting exceptional reactivity, generality, stability, and usability, TFMU-ADPr is an effective tool for evaluating small-molecule inhibitors and investigating the regulation of ADP-ribosyl catabolic enzymes in vitro [1].

Z-(L-Arg)-AMC is a fluorogenic substrate for trypsin, cathepsin B, and cathepsin H.1,2Upon enzymatic cleavage by trypsin, cathepsin B, or cathepsin H, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify trypsin, cathepsin B, and cathepsin H activity. AMC displays excitation emission maxima of 340-360 440-460 nm, respectively. 1.Zimmerman, M., Ashe, B., Yurewicz, E.C., et al.Sensitive assays for trypsin, elastase, and chymotrypsin using new fluorogenic substratesAnal. Biochem.78(1)47-51(1977) 2.Brindley, P.J., Kalinna, B.H., Dalton, J.P., et al.Proteolytic degradation of host hemoglobin by schistosomesMol. Biochem. Parasitol.89(1)1-9(1997)

Peroxyfluor 1 is a cell-permeable, first-generation green-fluorescent probe for H2O2 [1].

EtS-DMAB (HClO-green), a selective fluorescent probe, enables the detection of hypochlorous acid (HOCl) with excitation and emission wavelengths of 440 nm (λex) and 610 nm (λem), respectively. This compound is utilized for imaging both exogenous and endogenous HOCl in live cells [1].

Ac-IETD-AMC, a fluorogenic substrate specific for caspase-8 and granzyme B with an acetyl (Ac) moiety, is commonly utilized to quantify caspase-8 activity [1].

AMC-GlcNAc (compound 1) serves as a potent fluorogenic probe for the detection and characterization of β-hexosaminidase enzyme activity, offering continuous monitoring through turn-on fluorescence. Its fluorescence signal remains stable across a broad pH spectrum (Ex=325 nm, Em=390 nm). This compound facilitates ratiometric fluorescence detection with heightened sensitivity and minimal background, suitable for screening recombinant dispersin B activity in E. coli cell lysate [1].

NEP, a turn-on fluorescent probe employing the intramolecular charge transfer (ICT) mechanism, facilitates the detection of vicinal dithiol-containing proteins (VDPs). Notably, NEP exhibits exceptional selectivity towards VDPs in live cells and in vivo and manifests a robust green fluorescence signal at λ ex /λ em =430/535 nm. Furthermore, NEP bears promise for the potential diagnosis and study of parkinsonism.

Fast-TRFS, a selective and superfast fluorogenic probe of thioredoxin reductase (TrxR), can be utilized for imaging TrxR activity in live cells [1].

DACM (N-(7-Dimethylamino-4-methyl-3-coumarinyl)maleimide) is a thiol-reactive fluorescent dye with an excitation wavelength (Ex) of 396 nm and an emission wavelength (Em) of 468 nm [1].

AC-green (VDP-green), a β-allyl carbamate fluorescent probe, excels at specifically imaging vicinal dithiol proteins (VDPs) within living systems, demonstrating significant sensitivity, particularly in detecting reduced bovine serum albumin (rBSA). With excitation and emission wavelengths of 400 nm and 475 nm respectively (λ ex λ em =400 475 nm), this probe maintains low toxicity while being highly suitable for sensing VDPs in living cells and zebrafish [1].

CytoCalcein Violet 500 is a fluorogenic dye used to assess cell viability. It is a cell-permeable dye that, upon entering cells, is cleaved by intracellular esterases. CytoCalcein Violet 500 is retained by living cells and displays excitation emission maxima of 405 500 nm, respectively.

LYCBX is a neuronal tracer, a fluorescent biotin derivative of Lucifer Yellow [1].

7-Hydroxycoumarin-3-carboxylic acid, SE is the amine-reactive succinimidyl ester of 7-Hydroxycoumarin-3-carboxylic acid. This coumarin is also increasingly used to label peptides, nucleotides and carbohydrates.

D-Ala-Lys-AMCA TFA is a proton-coupled oligopeptide transporter 1 (PEPT1) substrate known for emitting blue fluorescence. Fluorescence analysis has shown that this compound can be transported into liver cancer cells and Caco-2 cells. It is useful for characterizing PEPT1-specific substrates or inhibitors [1] [2].