Benzenepentacarboxylic Acid (Pentacarboxybenzene) is a novel fluorescent probe to detect and scavenge HO-. Benzenepentacarboxylic acid is utilized to prove and detect the generation of HO- in H2O2/TAED and H2O2/TBCC alkali systems.

Benzenepentacarboxylic Acid labeled HO experiment
a. Solution preparation:
1. Mother solution preparation: Prepare a certain concentration of Benzenepentacarboxylic Acid mother solution, store it at -20℃ or -80℃ in the dark after aliquoting.
2. Working solution preparation: Dilute Benzenepentacarboxylic Acid to 400 μmol/L working solution, select the appropriate working solution concentration according to the experimental requirements, and try to prepare it before use.
b. Operation steps:
1. Mix the Benzenepentacarboxylic Acid working solution with other reaction reagents and react at a certain temperature for 60 min.
2. Rapidly cool the temperature, measure the fluorescence intensity at the maximum excitation wavelength (311 nm) and characterize the concentration of HO.