red fluorescent probe

DDAO is a promising near-infrared (NIR) red fluorescent probe with a long emission wavelength (λem = 656 nm) and a tunable excitation wavelength (600-650 nm). It can be designed for detecting the activities of various enzymes such as β-galactosidase, sulfatase, protein phosphatase 2A, carboxylesterase 2, human albumin, and esterases.

MitoSOX Red is a novel fluorescent probe that is specific and cell membrane-permeable and targets mitochondria in living cells.MitoSOX Red is oxidized by superoxide upon entry into the mitochondria and is not oxidized by other ROS or RNS-generating systems.MitoSOX Red then binds to nucleic acids in the mitochondrion and produces a strong red fluorescence.MitoSOX Red can be used as a fluorescent indicator for the specific detection of superoxide.MitoSOX Red can be used as a fluorescent indicator for the specific detection of superoxide. MitoSOX Red can be used as a fluorescent indicator to specifically detect superoxide. Superoxide dismutase (SOD) prevents the oxidation of MitoSOX Red.

MitoSOX Red TFA (Mito-hydroethidine TFA) is a fluorescent probe that specifically targets mitochondria in living cells.MitoSOX Red is susceptible to oxidation by superoxide, but is not easily oxidized by other ROS or RNS-generating systems.MitoSOX Red acts as a fluorescent indicator and can be used for live cell imaging.

ATP-Red 1 is a fluorescent probe with good membrane permeability that selectively and rapidly responds to intracellular ATP concentrations in live cells.

2-Hydroxy nile red trifluoromethanesulfonate is a fluorescent probe that can be incorporated into oligonucleotides as a nucleotide substitute. It serves as a DNA probe to detect polarity changes in the tumor microenvironment.

Alexa Fluor 594 azide is a bright red fluorescent azide-activated probe that reacts with terminal alkynes via copper-catalyzed click reactions (CuAAC) for single-molecule imaging.

HB-2-30 is a far-red fluorescent transglutaminase 2 (TG2) probe for in vitro and in vivo endocytosis imaging. HB-2-30 complexes with TG2 and α2-macroglobulin for efficient endocytosis via the LRP1 pathway.

Calcein Red AM is a fluorogenic dye that is used to assess cell viability. As the acetoxymethyl ester, Calcein Red AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein Red by intracellular esterases. Calcein Red is retained by living cells and displays excitation emission maxima of 560 574 nm, respectively. Calcein Red AM is a red-emitting variant of the green fluorophore calcein AM , which has been used to evaluate cell viability and cytotoxicity.

NIAD-4 is a fluorescent probe that crosses the blood-brain barrier to bind with high affinity to amyloid-β (Aβ) plaques (Ki = 10 nM). It displays excitation emission spectra of 475 625 nm, respectively, with the far-red emission occurring upon binding to Aβ.

Resorufin benzyl ether is a fluorometric probe that acts as a substrate for cytochrome P450 (CYP)3A4. It is typically used near its apparent Km value of 30 μM to screen the inhibition activation potential of test compounds, to predict potential drug-drug interactions, or to monitor other CYP450 activities. Upon enzyme cleavage, resorufin benzyl ether generates a red fluorescent product (ex. 571 nm em. 585 nm).

RB-OPD, also known as NO-red, is a fluorescent probe that combines o-phenylenediamine (OPD) and rhodamine nitric oxide (NO). With a λex of 550 nm and a λem of 590 nm, it exhibits exceptional sensitivity and selectivity.

LysoTracker Red (LysoTracker Red DND-99) is a fluorescent probe compound for lysosomal capture, a transport substrate for P-glycoprotein, with a maximum excitation emission wavelength of 577 590 nm.LysoTracker Red can be used for the long-term tracking of acidic vesicles.

Trx-red (NBL-SS perchlorate) is a red-emitting fluorescent probe derived from the Nile blue fluorophore, specifically designed for imaging thioredoxin (Trx) in live cells and in vivo, with excitation and emission wavelengths of 615 nm and 661 nm, respectively.

BSB ((trans,trans)-1-Bromo-2,5-bis-(3-hydroxycarbonyl-4-hydroxy)styrylbenzene) is a Congo red-derived fluorescent probe, an amyloid protein imaging agent, capable of interacting with amyloid fibrils formed by rat Aβ (1-40) peptide and selectively binding to compact plaques formed by prion proteins (PrP), used in studying neurodegenerative diseases.

4-Di-16-ASP, a green fluorescent membrane dye, exhibits lipophilic properties and functions as an aminostyryl probe with a broad emission spectrum, detectable through green, orange, or red filters. This compound is frequently utilized in neuronal membrane tracing, offering quicker diffusion compared to DiO [1].

OBI, a red-fluorescent probe designed for Red Broccoli, facilitates the detection of this RNA-based fluorophore in live mammalian cells, allowing for the monitoring of intracellular metabolites [1].

5-Nitro BAPTA, a calcium chelator, combined with 2-Me-substituted TM (as a fluorescent moiety), forms a red fluorescent probe (CaTM-2 AM) for imaging cytoplasmic Ca 2+ in cultured living cells. It serves as a building block for synthesizing Ca 2+ specific chelators, Ca 2+ buffers, and fluorescent Ca 2+ indicators [1] [2].

5-Nitro BAPTA tetramethyl ester, a calcium chelator, is involved in the synthesis of two-photon probes and is used for real-time imaging of intracellular calcium ions and monitoring calcium waves at depths of 100-300 μm in liver tissues for 1100-4000 s. Additionally, in conjunction with fluorescent compound 2-Me-substituted TM, it can form a red fluorescent probe (CaTM-2 AM) [1] [2].

Bella Fluor 594 Phalloidin, a photostable red-fluorescent probe, exhibits a high affinity for F-actin (Excitation Emission=581 609 nm) and is utilized for imaging cytoskeletal proteins [1] [2].

Fura Red AM, a fluorescent probe, is utilized for visualizing cytosolic [Ca(2+)] distribution in living plant tissue under an excitation wavelength of 488 nm [1].

JF646-Hoechst, with an excitation emission maximum of 655 670 nm [1], serves as an excellent red fluorescent DNA probe. It is a suitable replacement for large oligonucleotide-coupled antibodies in PAINT experiments, particularly within bacterial research contexts.

Nuclear Fast Red, an anthraquinone dye, serves primarily as a red nuclear counterstain in the presence of excess aluminum ions. Additionally, it functions as a histochemical and colorimetric reagent for calcium [1] and acts as a highly sensitive off on fluorescent probe for detecting guanine [2].

Quinaldine red functions as a fluorescent probe to determine the melting temperature (Tm) of proteins [1].

RhoNox-1 is a divalent iron ion (Fe²⁺) fluorescent probe specific and available for use in living cells, with a maximum absorption wavelength of 540 nm and a maximum emission wavelength of 575 nm, producing an irreversible orange-red fluorescent product. RhoNox-1 has the advantage of cell membrane permeability and is usually localized to the c.