red fluorescent probe

DDAO is a promising near-infrared (NIR) red fluorescent probe with a long emission wavelength (λem = 656 nm) and a tunable excitation wavelength (600-650 nm). It can be designed for detecting the activities of various enzymes such as β-galactosidase, sulfatase, protein phosphatase 2A, carboxylesterase 2, human albumin, and esterases.

JC-1 (CBIC2) is a fluorescent lipophilic cyanine dye. JC-1 is used to measure mitochondrial membrane potential. When the mitochondrial membrane potential is high, JC-1 aggregates into polymers within the matrix, emitting red fluorescence (λex=585 nm, λem=590 nm). When the mitochondrial membrane potential is low, JC-1 fails to aggregate within the mitochondrial matrix and exists as monomers, emitting green fluorescence (λex=510 nm, λem=527 nm).

MitoSOX Red is a fluorescent dye serving as a mitochondria-targeted fluorescent probe for superoxide, with cell membrane permeability and specificity. Upon oxidation, MitoSOX Red subsequently binds to nucleic acids within mitochondria/nuclei, producing intense red fluorescence, and is employed for detecting mitochondrial superoxide in live cells.

DAF-2 DA (5,6-Diaminofluorescein diacetate) is a cell-permeable fluorescent probe used for detecting nitric oxide (NO), requiring use in a serum-free environment free from phenol red interference. It has an excitation wavelength of 491 nm and an emission wavelength of 513 nm, with a commonly used working concentration of 1–10 μm.

Nuclear Fast Red is an anthraquinone dye that serves as a red nuclear counterstain when aluminum ions are present in excess, thereby aiding in histological visualization of nuclear components. Nuclear Fast Red additionally functions as a histochemical and colorimetric reagent for calcium detection,Nuclear Fast Red further acts as a highly sensitive “off/on” fluorescent probe for the detection of guanine, making it a multifunctional research reagent.

MitoSOX Red TFA (Mito-hydroethidine TFA) is a fluorescent probe that specifically targets mitochondria in living cells.MitoSOX Red is susceptible to oxidation by superoxide, but is not easily oxidized by other ROS or RNS-generating systems.MitoSOX Red acts as a fluorescent indicator and can be used for live cell imaging.

ATP-Red 1 is a fluorescent probe with good membrane permeability that selectively and rapidly responds to intracellular ATP concentrations in live cells.

2-Hydroxy nile red trifluoromethanesulfonate is a fluorescent probe that can be incorporated into oligonucleotides as a nucleotide substitute. It serves as a DNA probe to detect polarity changes in the tumor microenvironment.

Alexa Fluor 594 azide is a bright red fluorescent azide-activated probe that reacts with terminal alkynes via copper-catalyzed click reactions (CuAAC) for single-molecule imaging.

HB-2-30 is a far-red fluorescent transglutaminase 2 (TG2) probe for in vitro and in vivo endocytosis imaging. HB-2-30 complexes with TG2 and α2-macroglobulin for efficient endocytosis via the LRP1 pathway.

BP Fluor 594 alkyne is a bright red fluorescent alkyne-activated probe frequently utilized for imaging biomolecules containing azides. It reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linkage.

BP Light 650 carboxylic acid is a vibrant far-red fluorescent dye, performing comparably or superiorly to other dyes [including BP Fluor 647 and Cy5 dyes], suitable for fluorescent applications. It serves as a molecular probe for labeling antibodies and other proteins, facilitating cellular imaging and various fluorescence detection techniques.

SiR-Hoechst is a far-red fluorescent DNA probe commonly utilized for live-cell time-lapse imaging. It exhibits minimal toxicity even at concentrations as high as 10-25 µM. However, at concentrations significantly below 1 µM, SiR-Hoechst can induce a DNA damage response and G2 arrest.

PMBD is a fluorescent probe targeting lysosomes (Lyso). It selectively and sensitively detects endogenous N-acylethanolamine amidase (NAAA), enabling real-time visual monitoring of endogenous NAAA in live cells. PMBD exhibits a maximum absorption peak at 350 nm. Following NAAA metabolism, the product AMBD shows a red-shifted maximum absorption peak at 450 nm, along with a distinct fluorescence emission signal at 550 nm.

RhFNMB is a dual-channel/dual-localization single-molecule fluorescent probe for ATP and HOCl. When combined with ATP, it shows a light red channel (λex = 520 nm, λem = 586 nm), while its combination with HOCl results in a dark red channel (λex = 620 nm, λem = 688 nm), and it features independent fluorescence responses.

Halo tag TMR HCL (2764890-88-0 Free base) is a red fluorescent dye composed of a HaloTag ligand and TMR (TAMRA). This probe binds rapidly and stably to Halo protein covalently with high specificity and affinity, with excitation/emission wavelengths of 550/576 nm.

HBmito Crimson is a cell-permeable red fluorescent probe specifically targeting the inner mitochondrial membrane (IMM). It exhibits high selectivity for the IMM, making it useful for specific fluorescent staining of live cell mitochondria.

AF555 NHS Ester is a bright orange-red fluorescent probe containing the AF555 fluorophore and an NHS active ester group. It efficiently forms covalent bonds with primary amines (R‑NH₂) in biomolecules, enabling stable labeling and tracking of target molecules. This reagent is suitable for fluorescent labeling of proteins, amine-modified oligonucleotides, and other amine-containing molecules. Its excitation/emission maxima are 555/572 nm, making it widely used in bioimaging, immunoassays, and other life science applications.

Alexa Fluor 647 C2-Maleimide 6C is a maleimide-functionalized derivative of Alexa Fluor 647, emitting far-red fluorescence with excitation/emission maxima around 651/670 nm. Its maleimide group specifically reacts with thiol (-SH) groups in proteins or peptides, enabling efficient labeling of cysteine residues and other thiol-containing molecules. This dye is widely used in cell biology, neuroscience, and disease research as a functional far-red fluorescent probe.

AF647 Me-tetrazine is a multifunctional fluorescent probe formed by the covalent coupling of the far-red fluorescent dye Alexa Fluor 647 (AF647) with a methyl tetrazine (Me-tetrazine) group (Ex/Em ≈ 647/668 nm). AF647 Me-tetrazine is widely used in research applications such as multicolor fluorescence imaging of live cells and living organisms, flow cytometry, and single-molecule tracking.