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Rhodamine 101 inner salt is a bright fluorescent dye with excitation and emission maxima at 565 nm and 595 nm, respectively. It is utilized in various biological applications, including fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy, and ELISA.

Acridine Orange 10-Nonyl Bromide is a mitochondrial fluorescent probe that binds to cardiolipin in the inner mitochondrial membrane, dependent on the mitochondrial membrane potential, with an excitation wavelength of 490 nm and an emission wavelength of 510–525 nm.

HMSiR680-Me is a bright and stable fluorescent dye designed for multicolor, time-lapse, and super-resolution imaging to observe acidic organelles. It is suitable for overnight time-lapse experiments and rapid continuous super-resolution microscopy imaging, maintaining imaging quality for at least 1000 frames. HMSiR680-Me can also be used with silicon rhodamine dyes in multiplexed super-resolution microscopy experiments, enabling the visualization of interactions between the mitochondrial inner membrane and lysosomes using a single excitation laser and simultaneous depletion technique.

Alexa fluor 647 maleimide is a water-soluble fluorescent dye that maintains stability under a broad range of physiological conditions. Alexa fluor 647 maleimide facilitates the covalent attachment of fluorescent groups to proteins through selective reaction with thiol groups, thereby enabling precise labeling. This labeling method offers both high sensitivity and specificity, allowing detection of proteins at very low concentrations. Alexa fluor 647 maleimide is widely applied in the study of protein expression and localization, and it can also be conjugated to antibodies for labeling cell surface antigens or specific proteins within tissues [Ex/Em(nm)=651/671].

Patent Blue V (Acid blue 1), a biological dye, serves as an intraocular stain in retinectomy—surgical removal of the inner limiting membrane (ILM). Its use facilitates the complete excision of premembrane structures in vitreoretinal surgeries. Spectral analysis reveals that Patent Blue V absorbs strongly below 450 nm and above 600 nm, exhibiting a blue-green hue. Additionally, it functions as a tracer in lymphangiography to guide the surgical removal of cancerous lymph nodes [1][2].

1-Stearoyl-2-arachidonoyl-sn-glycero-3-phosphorylethanolamine (SAPE) is a natural phospholipid found in mitochondrial inner membranes, involved in lipid signaling.

DiSBAC10, a voltage-sensitive fluorescent probe, facilitates cell membrane electrical activity investigation in FRET assays. At rest, it populates the membrane's outer leaflet, accepting photons from excited fluorescein-labeled proteins and emitting them at higher wavelengths. Upon cell depolarization, DiSBAC10 quickly translocates to the inner leaflet, increasing fluorophore separation and diminishing the FRET signal.

Mito Flipper-TR 27, a fluorescent probe, accumulates in mitochondria to measure membrane tension in living cells. Its accumulation is driven by the strong internal negative membrane potential, which propels the permanently hydrophobic triphenylphosphine cation across the inner membrane and prevents its release, thereby facilitating mitochondrial tracking [1].