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Necrostatin 2

Name: Necrostatin 2
Brand: TargetMol
SKU: T12203L
Rating: 4.5 (1 reviews)

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Catalog No. T12203LCas No. 852391-19-6

Alias Necrostatin2

Necrostatin 2 is a necrotic protein and receptor-interacting protein kinase 1 (RIPK1) inhibitor used to study inner ear disorders such as sudden deafness, dizziness and tinnitus.

Necrostatin 2

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Purity: 99.92%

Catalog No. T12203LAlias Necrostatin2Cas No. 852391-19-6

Necrostatin 2 is a necrotic protein and receptor-interacting protein kinase 1 (RIPK1) inhibitor used to study inner ear disorders such as sudden deafness, dizziness and tinnitus.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$57	5 days	5 days
2 mg	$78	-	In Stock
5 mg	$166	5 days	5 days
1 mL x 10 mM (in DMSO)	$148	5 days	5 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.92%

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COA

Product Introduction

Bioactivity

Description	Necrostatin 2 is a necrotic protein and receptor-interacting protein kinase 1 (RIPK1) inhibitor used to study inner ear disorders such as sudden deafness, dizziness and tinnitus.
Targets&IC50	Jurkat cells (FADD-deficient human ):50 nM(EC50)
In vitro	Necrostatin 2 displays activity in a broad range of necroptosis cellular systems. [1] Necrostatin 2 at 30 μM fully protects L929 cells from TNF-α-induced necroptosis. [2] Necrotic apoptosis inhibitory activity was assessed by treating FADD-deficient human Jurkat T cells with TNF-α. Utilizing these conditions the cells efficiently undergo necroptosis, which is completely and selectively inhibited by Necrostatin 2 (EC50=50 nM). [3]
Synonyms	Necrostatin2

Chemical Properties

Molecular Weight	277.71
Formula	C₁₃H₁₂ClN₃O₂
Cas No.	852391-19-6
Smiles	C(C=1C=2C(NC1)=C(Cl)C=CC2)[C@@H]3C(=O)N(C)C(=O)N3
Relative Density.	1.450 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL(288.07 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (11.88 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.6009 mL	18.0044 mL	36.0088 mL	180.0439 mL
5 mM	0.7202 mL	3.6009 mL	7.2018 mL	36.0088 mL
10 mM	0.3601 mL	1.8004 mL	3.6009 mL	18.0044 mL
20 mM	0.1800 mL	0.9002 mL	1.8004 mL	9.0022 mL
50 mM	0.0720 mL	0.3601 mL	0.7202 mL	3.6009 mL
100 mM	0.0360 mL	0.1800 mL	0.3601 mL	1.8004 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc