biotinylation

DBCO-PEG4-Biotin (ADIBO-NH-PEG2-Biotin) is an azadibenzocyclooctyne-biotin derivative containing a biotin moiety and 4 PEGs.DBCO-PEG4-Biotin is a versatile biotinylation reagent that introduces a portion of biotin into an azide-labelled biomolecule via a copper-free catalytic alkyl azide reaction (SPAAC). azide-labelled biomolecules via a copper-free catalytic alkyl azide reaction (SPAAC).

N-Biotinyl-6-aminohexanoic acid (N-(+)-Biotinyl-6-aminohexanoic acid) is utilized for N-terminal biotinylation.

Biotin-DADOO (Biotinyl-3,6-dioxaoctanediamine) is a biotinylation reagent that can be used to synthesize a biotin-estradiol conjugate(i.e., biotin-DADOO-estradiol) to develop a direct, broad-range enzyme immunoassay to measure plasma estradiol concentrations.

Biotin Hydrazide is a carbonyl-reactive biotinylation reagent and carbonyl probe, ideal for labeling mouse monoclonal antibodies, lectins, sugars, unpaired cytosine residues in nucleic acids, and glycoproteins.

Sulfo-NHS-Biotin (Biotin-NHS) is a water-soluble biotinylation reagent used in Biotin-Streptavidin binding reactions. Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins in Streptavidin-Biotin assays. It can be used for protein detection or immobilization when used in conjunction with streptavidin and Immobilized Streptavidin.

Biotin-HPDP (Pyridyldithiol-biotin) is a sulfhydryl reactive biotinylation reagent.

Sulfosuccinimidyl-6-(biotinamido)hexanoate sodium is a biotinylation reagent and protein crosslinker capable of attaching biotin to the primary amine groups of proteins or other biomolecules.

(+)-Biotin 4-amidobenzoic acid is a substrate of biotinidase, which cleaves biotin amide to give biotin in vivo. Biotin is an essential coenzyme for certain carboxylases and is used to modify histones and regulate gene transcription. Biotinidase has key roles in intestinal absorption of biotin, the transport of biotin in plasma, and in the regulation of histone biotinylation. (+)-Biotin 4-amidobenzoic acid is hydrolyzed by biotinidase to release 4-aminobenzoic acid (PABA), which can be quantified by either fluorescent or colorimetric methods. This approach is used in biotinidase activity assays in newborn screening protocols to evaluate biotinidase deficiency.

Biotin-β-Amyloid (1-40) is an amyloid-β-(1-40) peptide with an N-terminal biotinylation.

Sulfo-NHS-LC-Biotin sodium, an amine-reactive biotin ester derivative, is incapable of traversing the intact blood-retinal barrier. Sulfo-NHS-LC-Biotin sodium is utilized to evaluate vascular permeability in cerebral and retinal vasculature.

Biotin-XX hydrazide, also known as Biotin-(AC5)2-hydrazide, is a carbonyl-reactive biotinylation reagent featuring two aminohexanoic acid spacers. This compound demonstrates a heightened efficacy in binding to avidin.

Biotin-LC-LC-NHS is a membrane permeable SMCC cross-linker and an NHS ester-activated biotinylation reagent that biotinylates antibodies or other proteins for detection or purification using streptavidin probes or resins.

Biotin-PEG7-Maleimide is a biotinylation agent that selectively binds to thiol (SH) groups and is used in synthesizing Drug-Linker Conjugates for Antibody-Drug Conjugates (ADCs) [1].

Biotin-crosstide is a derivative of the peptide Akt substrate crosstide, featuring biotinylation.

Biotin-PEG-NH2 (Mn 3700) is utilized for the biotinylation reaction of polymeric worm micelles, facilitating targeted delivery and drug transfer to cells.

Biotin-PEG-NH2 (Mn 5300) can be utilized in the biotinylation of polymer worm micelles to facilitate targeted delivery and drug transport into cells.

DSPE-PEG-Biotin, MW 3400, is a PEGylated lipid employed for biotinylation.

Biotin-PEG3-CoenzymeA is a non-cell-permeable substrate based on biotin with a PEG linker. It can be utilized for biotinylation in living cells, labeling in solutions, and interaction studies.

NHS-PEG4-biotinidase resistant biotin is a biotinylation reagent featuring a terminal NHS ester. The hydrophilic PEG spacer enhances its solubility in aqueous media. NHS ester is suitable for labeling primary amines (-NH2) found in proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Biotinyl-8-amino-3,6-dioxaoctanoic acid is a biotinylation reagent linked to a PEG chain, enhancing water solubility. Biotin acts as an affinity ligand suitable for biochemical applications such as pull-down assays or binding with streptavidin proteins. The carboxyl group can react with amine-containing molecules in the presence of activators like HATU.

Mal-Cyclohexyl-PEG3-Biotin is a PEG linker that includes a maleimide moiety and a biotin group. The maleimide specifically reacts with thiols at a pH between 6.5 and 7.5, forming a stable thioether bond. Biotinylation can occur with amine molecules in the presence of activators like EDC or HATU. The PEG linkage, attached to biotin, creates an extended spacer arm, facilitating biotin's access to protein binding sites. Additionally, the PEG component enhances the solubility of the biotin-PEG conjugate significantly.

Bis-PEG3-biotin is a biotinylation reagent featuring two terminal biotin groups. The PEG linkage forms an extended spacer arm that facilitates the biotin's access to binding sites on proteins or macromolecules.

Biotin-PEG5-OH (Biotin-PEG5-alcohol) is a biotinylation reagent featuring a primary hydroxyl group that can be derivatized. Compared to reagents with only hydrocarbon spacers, the PEG spacer in this compound enhances the solubility of labeled molecules.