biotinylation

DBCO-PEG4-Biotin (ADIBO-NH-PEG2-Biotin) is an azadibenzocyclooctyne-biotin derivative containing a biotin moiety and 4 PEGs.DBCO-PEG4-Biotin is a versatile biotinylation reagent that introduces a portion of biotin into an azide-labelled biomolecule via a copper-free catalytic alkyl azide reaction (SPAAC). azide-labelled biomolecules via a copper-free catalytic alkyl azide reaction (SPAAC).

N-Biotinyl-6-aminohexanoic acid (N-(+)-Biotinyl-6-aminohexanoic acid) is utilized for N-terminal biotinylation.

Biotin-DADOO (Biotinyl-3,6-dioxaoctanediamine) is a biotinylation reagent that can be used to synthesize a biotin-estradiol conjugate(i.e., biotin-DADOO-estradiol) to develop a direct, broad-range enzyme immunoassay to measure plasma estradiol concentrations.

Biotin Hydrazide is a carbonyl-reactive biotinylation reagent and carbonyl probe, ideal for labeling mouse monoclonal antibodies, lectins, sugars, unpaired cytosine residues in nucleic acids, and glycoproteins.

Sulfo-NHS-Biotin (Biotin-NHS) is a water-soluble biotinylation reagent used in Biotin-Streptavidin binding reactions. Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins in Streptavidin-Biotin assays. It can be used for protein detection or immobilization when used in conjunction with streptavidin and Immobilized Streptavidin.

Biotin-HPDP (Pyridyldithiol-biotin) is a sulfhydryl reactive biotinylation reagent.

Sulfosuccinimidyl-6-(biotinamido)hexanoate sodium is a biotinylation reagent and protein crosslinker capable of attaching biotin to the primary amine groups of proteins or other biomolecules.

(+)-Biotin 4-amidobenzoic acid is a substrate of biotinidase, which cleaves biotin amide to give biotin in vivo. Biotin is an essential coenzyme for certain carboxylases and is used to modify histones and regulate gene transcription. Biotinidase has key roles in intestinal absorption of biotin, the transport of biotin in plasma, and in the regulation of histone biotinylation. (+)-Biotin 4-amidobenzoic acid is hydrolyzed by biotinidase to release 4-aminobenzoic acid (PABA), which can be quantified by either fluorescent or colorimetric methods. This approach is used in biotinidase activity assays in newborn screening protocols to evaluate biotinidase deficiency.

Biotin-β-Amyloid (1-40) is an amyloid-β-(1-40) peptide with an N-terminal biotinylation.

Sulfo-NHS-LC-Biotin sodium, an amine-reactive biotin ester derivative, is incapable of traversing the intact blood-retinal barrier. Sulfo-NHS-LC-Biotin sodium is utilized to evaluate vascular permeability in cerebral and retinal vasculature.

Biotin-XX hydrazide, also known as Biotin-(AC5)2-hydrazide, is a carbonyl-reactive biotinylation reagent featuring two aminohexanoic acid spacers. This compound demonstrates a heightened efficacy in binding to avidin.

Biotin-LC-LC-NHS is a membrane permeable SMCC cross-linker and an NHS ester-activated biotinylation reagent that biotinylates antibodies or other proteins for detection or purification using streptavidin probes or resins.

Biotin-PEG7-Maleimide is a biotinylated biochemical assay reagent, which can be used as a thiol-specific probe. Cysteine-linked ADCs prepared with N-aryl maleimides exhibit less than 20% deconjugation in thiol-containing buffer Cysteine-linked ADCs prepared with N-aryl maleimides therefore markedly enhanced stability when compared with analogous ADCs prepared using N-alkyl maleimides, which undergo 35–67% deconjugation under identical conditions. Cysteine-linked ADCs prepared with N-aryl maleimides and incorporating the anticancer drug N-phenyl maleimide monomethyl-auristatin-E (MMAE) maintain strong cytotoxicity following prolonged serum exposure, whereas N-alkyl maleimide MMAE ADCs progressively lose potency.

Biotin-crosstide is a derivative of the peptide Akt substrate crosstide, featuring biotinylation.

Biotin-PEG-NH2 (Mn 3700) is utilized for the biotinylation reaction of polymeric worm micelles, facilitating targeted delivery and drug transfer to cells.

Biotin-PEG-NH2 (Mn 5300) can be utilized in the biotinylation of polymer worm micelles to facilitate targeted delivery and drug transport into cells.

DSPE-PEG-Biotin, MW 3400, is a PEGylated lipid employed for biotinylation.

Biotin-PEG-COOH (MW 3400) is a linear heterobifunctional PEG compound featuring biotin and a carboxylic acid functional group. The biotin moiety can bind with high specificity and affinity to avidin and streptavidin. In the presence of a coupling agent, Biotin-PEG-COOH (MW 3400) can be used for biotinylation of antibodies, proteins, and other macromolecules containing primary amines.

Biotin-PEG-CHO (MW 3400) is a biotin-conjugated PEG derivative employed for the biotinylation of biomolecules or the surfaces of other materials. Biotin is easily detectable through biotin/streptavidin affinity assays and is extensively applied in molecular targeting detection.

Biotin-PEG-COOH (MW 1000) is a linear heterobifunctional PEG compound characterized by biotin and carboxylic acid functional groups. The biotin moiety facilitates binding with avidin and streptavidin through high specificity and affinity. In the presence of coupling agents, Biotin-PEG-COOH (MW 1000) can be used for biotinylation of antibodies, proteins, and other macromolecules containing primary amines.

DBCO-PEG-Biotin (MW 2000) is a clickable biotinylation compound that enables copper-free click chemistry with azide molecules, resulting in the formation of stable triazole bonds.

DBCO-PEG-Biotin (MW 5000) is a click chemistry biotinylation reagent that can perform copper-free cycloaddition with azide-containing molecules, resulting in a stable triazole bond.

mPEG-Biotin (MW 5000) is a biotin-conjugated PEG derivative used for the biotinylation of biomolecules or material surfaces. Biotin can be detected through biotin/streptavidin binding assays, and it is widely utilized for molecular target detection.

Biotin-PEG3-CoenzymeA is a non-cell-permeable substrate based on biotin with a PEG linker. It can be utilized for biotinylation in living cells, labeling in solutions, and interaction studies.