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Biotin Hydrazide

Name: Biotin Hydrazide
Brand: TargetMol
SKU: T14578
Price: 48 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T14578Cas No. 66640-86-6

Biotin Hydrazide is a carbonyl-reactive biotinylation reagent and carbonyl probe, ideal for labeling mouse monoclonal antibodies, lectins, sugars, unpaired cytosine residues in nucleic acids, and glycoproteins.

Biotin Hydrazide

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Purity: 99.68%

Catalog No. T14578Cas No. 66640-86-6

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
100 mg	$51	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$48	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.68%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	Biotin Hydrazide is a carbonyl-reactive biotinylation reagent and carbonyl probe, ideal for labeling mouse monoclonal antibodies, lectins, sugars, unpaired cytosine residues in nucleic acids, and glycoproteins.
In vitro	Biotin Hydrazide is a biotinylation reagent. Which is used to biotinylate glycoproteins and glycolipids[1].

Chemical Properties

Molecular Weight	258.34
Formula	C₁₀H₁₈N₄O₂S
Cas No.	66640-86-6
Smiles	NNC(=O)CCCCC1SCC2NC(=O)NC12
Relative Density.	1.243 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 2 mg/mL (7.74 mM), Sonication is recommended.

DMSO: 13.5 mg/mL (52.26 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (3.87 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.8709 mL	19.3543 mL	38.7087 mL	193.5434 mL
5 mM	0.7742 mL	3.8709 mL	7.7417 mL	38.7087 mL

DMSO

	1mg	5mg	10mg	50mg
10 mM	0.3871 mL	1.9354 mL	3.8709 mL	19.3543 mL
20 mM	0.1935 mL	0.9677 mL	1.9354 mL	9.6772 mL
50 mM	0.0774 mL	0.3871 mL	0.7742 mL	3.8709 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc