b16-f10

Methyl myristate (methyl tetradecanoate) is a natural product.

Calcitriol (1,25-Dihydroxyvitamin D3) is a metabolite of vitamin D and a vitamin D receptor (VDR) agonist (IC50=0.4 nM). Calcitriol increases intestinal absorption of calcium and phosphorus, and increases bone resorption with parathyroid hormone.

Ursolic acid (Prunol) is a natural product, a pentacyclic triterpene carboxylic acid extracted from Rhododendron caprifolium. Ursolic acid has anti-tumor, anti-inflammatory, anti-bacterial and hypoglycemic activities.

Capsaicin is an active natural component found in chili peppers and acts as a TRPV1 agonist (EC50 = 0.29 μM). It possesses multiple activities, including pain relief, antitumor, anti-inflammatory, antioxidant, and neuroprotective effects, as well as some neurotoxicity. Capsaicin can be used to establish itch models.

L-Kynurenine ((S)-Kynurenine) is an endogenous agonist of the tryptophan metabolite and the aromatic hydrocarbon receptor (AHR). L-Kynurenine can inhibit the proliferation of allogeneic T cells and increase the invasion of malignant U87 glioma cells into the collagen matrix.

ALPHA-PINENE ((-)-Alpha-Pinene) is a bicyclic monoterpene found in pine trees and other plants, including Cannabis with diverse biological activities [1]. It reduces the growth of a panel of seven Gram-positive bacteria, seven Gram-negative bacteria, and eight yeast strains with MIC values of 0.75-1.29, 1.05-1.59, and 0.7-1.17%, respectively [2]. It has insecticidal activity against C. molestus larvae with LC50 values ranging from 47 to 49 mg/L.3 ALPHA-PINENE (100 μg/ml) induces apoptosis, increases anion superoxide production and DNA fragmentation, and activates caspase-3 in B16/F10 melanoma cells [4]. In a B16/F10 mouse xenograft model, ALPHA-PINENE(100 ml of a 10 mg/ml solution) reduces the number of metastatic lung nodules by approximately 7-fold. ALPHA-PINENE(8.6 mg/L, aerosol) also increases the time spent in the open arms of the elevated plus maze by approximately 2-fold in mice, indicating anxiolytic-like activity [5].

Eponemycin is an antibiotic with antitumor properties. It exhibits cytotoxicity against cancer cell lines B16-F10, L1210, P388, and HCT-116, with IC50 values of 0.0017, 0.01, 0.031, and 0.0097 µg/mL, respectively. In B16-F10 and mitomycin C, Eponemycin inhibits DNA synthesis, with IC50 values of 0.1 µg/mL and 0.41 µg/mL, respectively.

Pradimicin Q is an antibiotic exhibiting cytotoxicity against human colon HCT-116 cells and mouse melanoma B16-F10 cells, with IC50 values of 75 μg/mL and 100 μg/mL, respectively.

γ-Eudesmol ((+)-γ-Eudesmol) is a compound that induces apoptosis through mitochondrial pathways. It binds to mitochondrial membrane proteins, causing depolarization of the mitochondrial membrane potential and activates the caspase cascade. This compound exhibits cytotoxicity against various tumor cell lines, such as HepG2 and B16-F10, with IC50 values ranging from 8.86 to 15.15 μg/mL. γ-Eudesmol holds potential for research in cancers including hepatocellular carcinoma and melanoma.

2-Oxopomolic acid shows cytotoxic activity, it shows a significant decrease in intracellular melanin content in B16-F10 cells, and in culture media melanin. 2-Oxopomolic acid shows radical-scavenging activities similar to that of the reference antioxidant

Fupenzic acid shows a significant decrease in intracellular melanin content in B16-F10 cells, and in culture media melanin.

Uvedalin shows cytotoxicity against HeLa, HL-60, and Murine B16-F10 melanoma cell lines.

(+)-Norglaucine shows cytotoxic activity toward the tumor cell lines B16-F10 (mouse melanoma), HepG2 (human hepatocellular carcinoma), K562 (human chronic myelocytic leukemia) and HL-60 (human promyelocytic leukemia) and non-tumor peripheral blood mononuc