Tomatidine hydrochloride (Tomatidine HCl) is a steriodal alkaloid structurally similar to Cyclopamine but does not inhibit hedgehog pathway. May be used as a negative control for Cyclopamine and KAAD-Cyclopamine. Tomatidine protects against muscle atrophy and boosting muscle growth.

Tomatidine serves as an anti-inflammatory agent by inhibiting NF-κB and JNK signaling pathways. Additionally, it induces autophagy in both mammalian cells and C. elegans.