thiol-groups

N-Succinimidyl-S-acetylthioacetate (SATA) is a protein modification agent that introduces thiol-groups into protein molecules and adds sulfhydryl groups in a protected form to proteins and other amine-containing molecules for later reaction with sulfhydryl reactive crosslinkers such as Sulfo-SMCC, Sulfo-MBS, etc.

DTNB (Ellman's Reagent) is used to quantify thiol groups in a sample and measure low-molecular-mass thiols, such as glutathione, in pure solutions and biological samples [such as blood].

DM3 (Maytansinoid DM3) is a maytansine mimic with disulfide or thiol groups, acting as a tubulin inhibitor and serving as the cytotoxic component in antibody-drug conjugates (ADCs).

Nitrosobiotin [4-keto-4-(6-(5-(311-(3,5-dihydroxyphenyl)-balanyl)-1-tetrahydro-2H-thiopyran-3-yl)amino)-2,4,6trihydroxyphen-1-yl]-is a cyclic monomeric lactone. It contains three carbonyl groups (one on each carbon) but without ethanol functionalities to connect a third thiol group.

MTSES sodium (Sodium (2-Sulfonatoethyl)methanethiosulfonate) is a negatively charged, membrane-impermeable methanethiosulfonate (MTS). This MTS compound can react with thiol groups to form mixed disulfides, frequently utilized in studying cysteine residues on proteins.

Thiol-PEG3-alcohol is a PEG derivative containing a hydroxyl group and a thiol group. The hydrophilic PEG spacer increases solubility in aqueous media. The hydroxyl group enables further derivatization or replacement with other reactive functional groups.

L-Cysteic acid is an endogenous metabolite and the oxidation product of L-cysteine, where the thiol group is completely oxidised to sulfonic acid/sulfonic acid groups, and can serve as a substrate for GADCase, with a Km of 5.4 mM.

Maleimide-PEG4-NHS is a sulfhydryl and amine reactive heterofuncational PEG linker. The chemical bonds formed through Maleimide-PEG4-NHS linker are stable and are not cleavable. The NHS ester reacts with amino groups at pH 7-9 to form stable amide bond wh

Bis-PEG5-thiol is a PEG derivative containing two thiol groups. The thiol groups react with maleimide, OPSS, vinylsulfone and transition metal surfaces including gold, silver, etc. The hydrophilic PEG spacer increases solubility in aqueous media.

Thiol-PEG2-acid is a PEG derivative containing a thiol group and a terminal carboxylic acid. The hydrophilic PEG spacer increases solubility in aqueous media. The thiol group reacts with maleimide, OPSS, vinylsulfone and transition metal surfaces including gold, silver, etc. The terminal carboxylic acid can be reacted with primary amine groups in the presence of activators (e.g. EDC, or DCC) to form a stable amide bond.

p-Aminophenylmercuric acetate (APMA) is an organic mercury compound and thiol-blocking agent commonly employed as an activator of MMPs (matrix metalloproteinases), capable of modulating cysteine-zinc ion interactions. APMA also induces platelet aggregation activation/inhibition in a dose-dependent manner.

Biotin-PEG7-Maleimide is a biotinylation agent that selectively binds to thiol (SH) groups and is used in synthesizing Drug-Linker Conjugates for Antibody-Drug Conjugates (ADCs) [1].

N,N-Bis(PEG2-N3)-N-amido-PEG2-thiol serves as a cleavable 4 unit PEG ADC linker in the preparation of antibody-drug conjugates (ADCs) [1]. This compound functions as a click chemistry reagent, equipped with an Azide group, allowing it to participate in copper-catalyzed azide-alkyne cycloaddition reactions (CuAAc) with alkyne-bearing molecules. Additionally, it is capable of engaging in strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules that contain DBCO or BCN groups.

N,N-Bis(PEG2-alkyne)-N-amido-PEG2-thiol is an ADC linker that integrates four PEG units and is used in creating antibody-drug conjugates (ADCs) [1]. This compound functions as a click chemistry reagent possessing an Alkyne group, which enables it to participate in copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules that contain Azide groups.

Thiol-PEG3-thiol (Bis-PEG3-thiol) is a crosslinker with thiol groups employed in the synthesis of PEG hydrogels.

Poly(ethylene glycol) methyl ether maleimide (Mn 2000) is a polyethylene glycol containing the maleimide (HY-W007324) group. With its maleimide functional group, this compound is utilized for conjugating biomolecules that contain thiol groups.

Dansylamidoethyl mercaptan is a molecule that reacts with the -SH groups of proteins and other thiol-containing molecules, and it is active in detecting disulfide bonds (S-S) in proteins.

Glutaraldehyde solution, 50% in H2O, is a solution consisting of 50% glutaraldehyde in water, often employed for cell fixation in staining experiments. Glutaraldehyde forms covalent bonds with nucleophilic groups such as amino and thiol groups in proteins, leading to irreversible cross-linking of intracellular proteins and effectively fixing cells. It stabilizes cell morphology and structure, facilitating subsequent observation and research. Additionally, glutaraldehyde solution can serve as a sterilizing agent, food processing aid, disinfectant, tanning agent, and wood preservative.

Biotin-PE-maleimide (N-Biotinyl-N'-[2-(N-maleimido)ethyl]piperazine) is a large, membrane-impermeable, thiol-containing reagent, suitable for biotin labeling (such as sulfhydryl groups) and for protein or other biomolecule detection.

6-Maleimidocaproic acid hydrazide (N-ε-maleimidocaproic acid hydrazi) TFA is a maleimide derivative and serves as a heterobifunctional crosslinker that is reactive towards carbonyl and thiol groups.

N-Boc-N'-(PEG1-t-butyl ester)-L-Lysine-amido-Mal is a lysine amino acid derivative with a maleimide group at the C-terminus. The α-amino group is protected by a Boc group, and the ε-amino group is capped with an amide-PEG1-t-butyl ester. The maleimide group is a thiol-reactive covalent moiety for attaching to cysteine residues, while the Boc and t-butyl ester groups can be deprotected for subsequent reactions.

Mal-PEG5-amine is a PEG linker that contains a maleimide group and an amine group. The hydrophilic PEG spacer enhances its solubility in aqueous media. The amine group can react with carboxylic acids, activated NHS esters, and carbonyl groups (ketones, aldehydes), while the maleimide group can form covalent bonds with thiol groups, allowing biomolecules to connect via thiol linkage.

Mal-PEG12-t-butyl ester is a PEG linker containing a maleimide group and a t-butyl ester group. The hydrophilic PEG spacer enhances solubility in aqueous media. The t-butyl-protected carboxyl group can be deprotected under acidic conditions. The maleimide group reacts with thiol groups to form covalent bonds, enabling the conjugation of biomolecules with thiols.

Mal-PEG4-amine, a PEG linker containing a maleimide group and an amine group, features a hydrophilic PEG spacer that enhances its solubility in aqueous media. The amine group can react with carboxylic acids, activated NHS esters, and carbonyls (ketones, aldehydes). The maleimide group reacts with thiol groups to form covalent bonds, enabling the conjugation of biomolecules with thiols.