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DTNB

Name: DTNB
Brand: TargetMol
SKU: T15177
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T15177Cas No. 69-78-3

Alias Ellman's Reagent

DTNB (Ellman's Reagent) is used to quantify thiol groups in a sample and measure low-molecular-mass thiols, such as glutathione, in pure solutions and biological samples [such as blood].

DTNB

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Purity: 99.51%

Catalog No. T15177Alias Ellman's ReagentCas No. 69-78-3

DTNB (Ellman's Reagent) is used to quantify thiol groups in a sample and measure low-molecular-mass thiols, such as glutathione, in pure solutions and biological samples [such as blood].

Pack Size	Price	USA Warehouse	Global Warehouse
1 g	$29	In Stock	In Stock
5 g	$52	-	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.51%

Resource Download

COA HPLC HNMR

Product Introduction

Bioactivity

Description	DTNB (Ellman's Reagent) is used to quantify thiol groups in a sample and measure low-molecular-mass thiols, such as glutathione, in pure solutions and biological samples [such as blood].
Synonyms	Ellman's Reagent

Chemical Properties

Molecular Weight	396.35
Formula	C₁₄H₈N₂O₈S₂
Cas No.	69-78-3
Smiles	C(O)(=O)C1=C(N(=O)=O)C=CC(SSC2=CC(C(O)=O)=C(N(=O)=O)C=C2)=C1
Relative Density.	1.67 g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 247.5 mg/mL (624.45 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.5 mg/mL (6.31 mM), Sonication is recommeded.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5230 mL	12.6151 mL	25.2302 mL	126.1511 mL
5 mM	0.5046 mL	2.5230 mL	5.0460 mL	25.2302 mL
10 mM	0.2523 mL	1.2615 mL	2.5230 mL	12.6151 mL
20 mM	0.1262 mL	0.6308 mL	1.2615 mL	6.3076 mL
50 mM	0.0505 mL	0.2523 mL	0.5046 mL	2.5230 mL
100 mM	0.0252 mL	0.1262 mL	0.2523 mL	1.2615 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc