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Fluorescein-maleimide is a fluorescein dye conjugate containing a maleimide group. In the pH range of 6.5 to 7.5, the maleimide components in fluorescein-maleimide react with thiol-containing compounds or biomolecules to form thioester bonds. This compound is specifically used for the fluorescent labeling of proteins, nucleic acids, or other molecules that have one or more thiol groups.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | 10-14 weeks | 10-14 weeks | |
| 50 mg | Inquiry | 10-14 weeks | 10-14 weeks |
| Description | Fluorescein-maleimide is a fluorescein dye conjugate containing a maleimide group. In the pH range of 6.5 to 7.5, the maleimide components in fluorescein-maleimide react with thiol-containing compounds or biomolecules to form thioester bonds. This compound is specifically used for the fluorescent labeling of proteins, nucleic acids, or other molecules that have one or more thiol groups. |
| Synonyms | 5-FITC-Maleimide |
| Molecular Weight | 529.52 |
| Formula | C27H19N3O7S |
| Cas No. | 2228857-33-6 |
| Smiles | O=C1OC2(C3=CC=C(O)C=C3OC4=CC(O)=CC=C42)C5=CC=C(C=C15)NC(=S)NCCN6C(=O)C=CC6=O |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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