tcep

TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a trialkylphosphine that selectively reduces proteolysis. TCEP hydrochloride is a non-thiol-reducing agent that promotes the binding of NF-κB-DNA.

TCEP-biotin is biotinylated form of the reducing agent TCEP and an affinity probe for protein lysine crotonylation. Covalent addition of the TCEP moiety to crotonylated lysine residues results in adduct formation that allows for detection or immobilization of crotonylated proteins through interaction with the biotin ligand. It has been used in the detection and isolation of crotonylated histones in HEK293 cells.

DBCO-S-S-PEG3-biotin is a cleavable reagent for the introduction of a biotin moiety to azide-containing biomolecules using copper-free Click Chemistry. The disulfide bond in this linker can be cleaved with reducing agents such as DTT, BME, and TCEP to remove the biotin label. PEG linkers can also be used in the synthesis of PROTACs.

PB1 is a highly-effective intracellular disulfide reducing agent with notable attributes such as excellent cell permeability, the capacity to generate a substantial intracellular concentration gradient, and remarkable stability. It serves as a borane-protected TCEP (tris(2-carboxyethyl)phosphine) analogue. PB1 demonstrates the potential to enhance the survival of retinal ganglion cells following axotomy in vitro at concentrations in the nanomolar and picomolar range. Consequently, PB1 has proven instrumental in the study of neuroprotective properties[1][2][3].

PB2, a tris(2-carboxyethyl)phosphine (TCEP) analogue, enhances the survival of retinal ganglion cells (RGCs) following axotomy in vitro. Even at nanomolar and picomolar concentrations, PB2 demonstrates pronounced efficacy in promoting RGC survival. Notably, PB2 exhibits superior permeability compared to TCEP. Serving as a potent reducing agent, PB2 provides robust neuroprotection for RGCs[1].