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TCEP hydrochloride

Name: TCEP hydrochloride
Brand: TargetMol
SKU: T7087
Price: 43 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T7087Cas No. 51805-45-9

Alias Tris(2-carboxyethyl)phosphine hydrochloride, TCEP HCl

TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a trialkylphosphine that selectively reduces proteolysis. TCEP hydrochloride is a non-thiol-reducing agent that promotes the binding of NF-κB-DNA.

TCEP hydrochloride

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Purity: 99.93%

Catalog No. T7087Alias Tris(2-carboxyethyl)phosphine hydrochloride, TCEP HClCas No. 51805-45-9

Pack Size	Price	USA Warehouse	Global Warehouse
100 mg	$43	In Stock	In Stock
500 mg	$54	In Stock	In Stock
1 g	$68	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$45	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products. If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Batch Information

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Purity:99.93%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a trialkylphosphine that selectively reduces proteolysis. TCEP hydrochloride is a non-thiol-reducing agent that promotes the binding of NF-κB-DNA.
Targets&IC50	MCF-7 cells:> 50 μM (GI50), WI-38 cells:> 50 μM (GI50), NS3/4A:12.8 μM, A549 cells:> 50 μM (GI50)
In vitro	METHODS: Human hepatocellular carcinoma cells HepG2 were treated with TCEP hydrochloride (100-400 µM) for 3 days, and cell viability was detected by MTT assay. RESULTS: The viability of HepG2 was significantly reduced to 25.68% and 70.92% after treatment with 200 and 400 µM TCEP for 3 days, whereas the lowest concentration of TCEP (100 µM) did not significantly reduce the viability of HepG2 (3.44%). [1] METHODS: Human peripheral blood mononuclear cell PBMCs were treated with TCEP hydrochloride (0.5-1 mM) for 24 h. Morphological and necrotic changes were assessed by Hoechst 33342/PI staining. RESULTS: Necrosis was observed in cells treated with 0.5 mM of TCEP. [2]
In vivo	METHODS: To investigate the neurotoxicity and its mechanism in mice, TCEP hydrochloride (10-100 mg/kg) was administered by gavage to Kunming mice once a day for 30 days. RESULTS: Compared with the control group, the high dose TCEP group showed a significant decrease in water intake and a significant increase in liver and spleen organ indices. In the water maze test, the escape latency of TCEP-exposed mice was longer than that of the control group, and the total swimming course of the high-dose TCEP group was higher than that of the control group, and the swimming time of the target quadrant was significantly shorter.TCEP exposure may cause neurotoxicity by increasing thyroid hormones and inducing oxidative damage in mice. [3]
Synonyms	Tris(2-carboxyethyl)phosphine hydrochloride, TCEP HCl

Chemical Properties

Molecular Weight	286.65
Formula	C₉H₁₆ClO₆P
Cas No.	51805-45-9
Smiles	Cl.OC(=O)CCP(CCC(O)=O)CCC(O)=O
Relative Density.	1.041 g/cm3 at 25℃

Storage & Solubility Information

Storage

store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 252.5 mg/mL (880.87 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 2.75 mg/mL (9.59 mM), Solution.

5% DMSO+95% Saline: 1.45 mg/mL (5.06 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.4886 mL	17.4429 mL	34.8857 mL	174.4287 mL
5 mM	0.6977 mL	3.4886 mL	6.9771 mL	34.8857 mL
10 mM	0.3489 mL	1.7443 mL	3.4886 mL	17.4429 mL
20 mM	0.1744 mL	0.8721 mL	1.7443 mL	8.7214 mL
50 mM	0.0698 mL	0.3489 mL	0.6977 mL	3.4886 mL
100 mM	0.0349 mL	0.1744 mL	0.3489 mL	1.7443 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc