bioorthogonal

Itaconate-alkyne (ITalk) is a bioorthogonal probe for quantitative, site-specific chemoproteomic profiling of itaconate in inflammatory macrophages, facilitating biochemical evaluation and proteomic analysis of its direct targets.

5-PT (5-Propargyltryptamide) is an alkynylated 5-HT analog that can undergo bioorthogonal click chemistry (e.g., azide-alkynyl cycloaddition reactions) used in neuroscience research as a tool compound or probe to track down proteins interacting with 5-PT and to explore the mechanism of 5-hydroxytryptamine's action in cells.

L-Homopropargylglycine is an alkyl chain-based PROTAC linker utilized in the synthesis of PROTACs[1].

DSPE-PEG(2000)-TCO is a PEGylated derivative of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) that features a trans-cyclooctene (TCO) moiety capable of reacting with tetrazine to facilitate bioorthogonal conjugation in lipid nanoparticles (LNPs). This compound's integration of the TCO group endows it with significant utility in biomedical research, particularly in the bioconjugation of lipid nanoparticles.

Difluorinated H2S probe 1 is a fluorescent probe for hydrogen sulfide (H2S).1It selectively fluoresces in the presence of H2S over Zn2+, Fe3+, S2O32-, ClO-, SO32-, H2O2, NO2-, cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) when used at a concentration of 1 μM. Difluorinated H2S probe 1 displays excitation/emission maxima of 365/450 nm, respectively. 1.Zhang, J., Gao, Y., Kang, X., et al.o,o-Difluorination of aromatic azide yields a fast-response fluorescent probe for H2S detection and for improved bioorthogonal reactionsOrg. Biomol. Chem.15(19)4212-4217(2017)

Difluorocyclooctyne-CH2-COOH, a difluorinated cyclooctyne (DIFO) analogue, facilitates imaging of glycans on live cells by rapidly reacting with azides without requiring copper catalysis. This characteristic makes it an effective tool for bioorthogonal conjugation in live cell studies.

Ac4GalNAlk, a weakly alkyne-labeled reagent for metabolic oligosaccharide engineering (MOE), facilitates the detection of protein glycosylation by promoting nucleotide-sugar biosynthesis and enhancing bioorthogonal cell surface markers. It is activated by cellular biosynthetic machinery into nucleotide sugars, subsequently enabling the tracing of glycoproteins in bioorthogonal chemistry [1].

Compound PA, also known as Pomalidomide-CO-C5-azide, serves as a ligand for E3 ligase and features a bioorthogonal azide group. This compound is utilized in the synthesis of PROTACs that exhibit anticancer properties [1].

H-L-Lys(4-N3-Z)-OH hydrochloride is a click chemistry reagent that features a lysine-modified azide moiety, serving as a bioorthogonal ligation handle. It functions both as an infrared probe and a photo-affinity reagent [1] [2]. This compound contains an azide group integral to these applications.

Fmoc-L-Lys(4-N3-Z)-OH is a chemical reagent characterized by its azide group. This compound serves as a lysine building block in solid-phase peptide synthesis (SPPS) with azide functionality, useful as a bioorthogonal handle, an infrared probe, and a photoaffinity label. It undergoes strain-promoted 1,3-dipolar cycloaddition facilitated by trans-cyclooctene decarboxylation. Additionally, Fmoc-L-Lys(4-N3-Z)-OH functions as a click chemistry reagent, enabling copper-catalyzed azide-alkyne cycloaddition reactions (CuAAc) with molecules containing alkyne groups. Moreover, it can participate in strain-promoted alkyne-azide cycloaddition reactions (SPAAC) with molecules that possess DBCO or BCN groups.

N3-D-Lys(Fmoc)-OH is a click chemistry reagent equipped with an azide group that facilitates highly specific bioorthogonal reactions: biocompatibility, rapid action, and extreme specificity in biological environments. This compound can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing an alkyne group. It is also capable of strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules that possess DBCO or BCN groups.