Biotin sodium

Biotin Sodium (Vitamin B7) is a water-soluble B vitamin acting as a coenzyme for five carboxylases critical in human metabolism, including the synthesis of fatty acids, isoleucine, and valine, as well as gluconeogenesis. It is essential for cell growth, fatty acid production, and the metabolism of fats and amino acids [1] [2] [3].

1,2-Dipalmitoyl-sn-glycero-3-PE-N-(cap biotin) is a biotinylated phospholipid. It has been used in PEGylated polyamidoamine-dendrimer-conjugated supported lipid bilayers (SLB) to isolate circulating tumor cells and tumor cell microembolis from patient-derived blood by antibody-coated microfluidics. [1] It has also been used as a component of SLBs to detect protein-ligand binding with ortho-conjugated Texas Red DHPE. [2] In addition, 1,2-dipalmitoyl-sn-glycero-3-PE-N-(cap biotin) has been used in SLBs partitioned into nanowells to create DNA curtains, which can be used as a high-throughput tool for detection of protein-DNA interactions at the single molecule level.[3]

(+)-Biotin 4-amidobenzoic acid is a substrate of biotinidase, which cleaves biotin amide to give biotin in vivo. Biotin is an essential coenzyme for certain carboxylases and is used to modify histones and regulate gene transcription. Biotinidase has key roles in intestinal absorption of biotin, the transport of biotin in plasma, and in the regulation of histone biotinylation. (+)-Biotin 4-amidobenzoic acid is hydrolyzed by biotinidase to release 4-aminobenzoic acid (PABA), which can be quantified by either fluorescent or colorimetric methods. This approach is used in biotinidase activity assays in newborn screening protocols to evaluate biotinidase deficiency.

Biotin-labeled ODN TTAGGG (sodium) is an inhibitory oligonucleotide (ODN) that functions as an antagonist to TLR9, AIM2, and cGAS. It serves as a tool for assessing cellular uptake and localization of CpG ODN using biotin detection systems and light microscopy.

Biotin-labeled Agatolimod (sodium), a class B CpG ODN (oligodeoxynucleotide) and TLR9 agonist, facilitates the assessment of cellular uptake and localization of CpG ODNs through a biotin detection system with light microscopy.

Biotin-labeled ODN 1826 (sodium), a class B CpG oligodeoxynucleotide (ODN), serves as a TLR9 agonist and facilitates assessment of CpG ODN cellular uptake and localization through biotin detection systems and light microscopy.

Biotin-labeled ODN 2216 (sodium) serves as a specific agonist for human TLR9 (toll-like receptor 9), facilitating the assessment of CpG ODN cellular uptake and localization through a biotin detection system coupled with light microscopy.

Biotin-labeled ODN 1018 (sodium), a TLR-9 agonist oligodeoxynucleotide, serves as a tool for assessing cellular uptake and localization of CpG ODNs through biotin detection systems and light microscopy.

Biotin-labeled ODN 2395 (sodium), a Class C oligodeoxynucleotide and TLR9 agonist, serves to assess cellular uptake and localization of CpG ODN through a biotin detection system coupled with light microscopy.

Sulfo-NHS-LC-Biotin sodium is an amine-reactive biotinylation reagent for antibody labeling.

Biotin-labeled ODN 1585 (sodium) serves as a potent inducer of IFN and TNFα production and facilitates the assessment of CpG ODN cellular uptake and localization through detection with a biotin system and light microscopy analysis.

Biotin-labeled ODN 1668 (sodium), a class B CpG oligodeoxynucleotide (ODN) and TLR9 agonist, facilitates the assessment of cellular uptake and localization of CpG ODNs via a biotin detection system and light microscopy.

Biotin-labeled ODN 2088 (sodium) acts as a potent inhibitor of TLR3, TLR7, and TLR9. It is utilized to assess cellular uptake and localization of CpG ODN through a biotin detection system coupled with light microscopy.

The PtdIn phosphates play an important role in the generation and transduction of intracellular signals. PtdIns-(3,4,5)-P3-biotin is an affinity probe which allows the PIP3 to be detected through an interaction with the biotin ligand. This design allows PtdIns-(3,4,5)-P3-biotin to serve as a general probe for any protein with a high affinity binding interaction with inositol-(3,4,5)-triphosphate phospholipids, such as phosphatidylinositol 3-kinase, PTEN, or PH-domain-containing proteins.