Biotin sodium

Biotin Sodium (Vitamin B7) is a water-soluble B vitamin acting as a coenzyme for five carboxylases critical in human metabolism, including the synthesis of fatty acids, isoleucine, and valine, as well as gluconeogenesis. It is essential for cell growth, fatty acid production, and the metabolism of fats and amino acids [1] [2] [3].

Sulfo-NHS-LC-Biotin sodium is an amine-reactive biotinylation reagent used for antibody labeling.

(+)-Biotin 4-amidobenzoic acid is a substrate of biotinidase, which cleaves biotin amide to give biotin in vivo. Biotin is an essential coenzyme for certain carboxylases and is used to modify histones and regulate gene transcription. Biotinidase has key roles in intestinal absorption of biotin, the transport of biotin in plasma, and in the regulation of histone biotinylation. (+)-Biotin 4-amidobenzoic acid is hydrolyzed by biotinidase to release 4-aminobenzoic acid (PABA), which can be quantified by either fluorescent or colorimetric methods. This approach is used in biotinidase activity assays in newborn screening protocols to evaluate biotinidase deficiency.

1,2-Dipalmitoyl-sn-glycero-3-PE-N-(cap biotin) is a biotinylated phospholipid. It has been used in PEGylated polyamidoamine-dendrimer-conjugated supported lipid bilayers (SLB) to isolate circulating tumor cells and tumor cell microembolis from patient-derived blood by antibody-coated microfluidics. [1] It has also been used as a component of SLBs to detect protein-ligand binding with ortho-conjugated Texas Red DHPE. [2] In addition, 1,2-dipalmitoyl-sn-glycero-3-PE-N-(cap biotin) has been used in SLBs partitioned into nanowells to create DNA curtains, which can be used as a high-throughput tool for detection of protein-DNA interactions at the single molecule level.[3]

The PtdIn phosphates play an important role in the generation and transduction of intracellular signals. PtdIns-(3,4,5)-P3-biotin is an affinity probe which allows the PIP3 to be detected through an interaction with the biotin ligand. This design allows PtdIns-(3,4,5)-P3-biotin to serve as a general probe for any protein with a high affinity binding interaction with inositol-(3,4,5)-triphosphate phospholipids, such as phosphatidylinositol 3-kinase, PTEN, or PH-domain-containing proteins.

Biotin-labeled Agatolimod (sodium), a class B CpG ODN (oligodeoxynucleotide) and TLR9 agonist, facilitates the assessment of cellular uptake and localization of CpG ODNs through a biotin detection system with light microscopy.

Biotin-labeled ODN 1826 (sodium), a class B CpG oligodeoxynucleotide (ODN), functions as a TLR9 agonist and enables the evaluation of CpG ODN cellular uptake and localization using biotin detection systems and light microscopy.

Biotin-labeled ODN 1018 (sodium), a TLR-9 agonist oligodeoxynucleotide, is used to evaluate cellular uptake and localization of CpG ODNs via biotin detection systems and light microscopy.

Biotin-labeled ODN 1585 (sodium) effectively induces IFN and TNFα production, and enables assessment of CpG ODN cellular uptake and localization via biotin system detection and light microscopy analysis.

Biotin-labeled ODN 1668 (sodium), a class B CpG oligodeoxynucleotide (ODN) and TLR9 agonist, enables the evaluation of cellular uptake and localization of CpG ODNs using a biotin detection system and light microscopy.

Biotin-labeled ODN 2088 (sodium) is a potent inhibitor of TLR3, TLR7, and TLR9, used to evaluate cellular uptake and localization of CpG ODN via a biotin detection system in conjunction with light microscopy.

Biotin-labeled ODN 2216 (sodium) acts as a specific agonist for human TLR9 (toll-like receptor 9), enabling the evaluation of CpG ODN cellular uptake and localization via a biotin detection system coupled with light microscopy.

Biotin-labeled ODN 2395 (sodium), a Class C oligodeoxynucleotide and TLR9 agonist, is used to evaluate cellular uptake and localization of CpG ODN via a biotin detection system integrated with light microscopy.

Biotin-labeled ODN TTAGGG (sodium) is an inhibitory oligonucleotide (ODN) that antagonizes TLR9, AIM2, and cGAS, serving as a tool for assessing cellular uptake and localization of CpG ODN using biotin detection systems and light microscopy.