ns2b-ns3

Compound 11, also known as NS2B-NS3pro-IN-2, functions as an inhibitor of the Zika Virus NS2B-NS3 protease and exhibits antiviral properties at a concentration of 50 μM [1].

NS2B-NS3pro-IN-1, a potent Zika Virus NS2B-NS3 protease inhibitor, exhibits an EC50 of 50 μM and is significant in the ZIKV replication cycle [1].

Palmatine chloride an isoquinoline alkaloid, is an important medicinal herbal extract with diverse pharmacological and biological properties.

ARDP0006 is an inhibitor of NS2B-NS3 protease.

1. Palmatine (Burasaine) is an inhibitor of dopamine generation. 2. Palmatine could potentially be developed for the treatment of flavivirus infections. 3. Palmatine has been used in the treatment of jaundice, dysentery, hypertension, inflammation, and liver-related diseases.

SID-852843 is a WNV NS2B-NS3 protease inhibitor with an IC50 value of 0.105 μM for WNV NS2B-NS3 protease and can be used for the study of viral infections.

ZINC03129319 is an inhibitor of dengue virus (DENV) NS2B-NS3 protease.

Compound 34e (NS2B/NS3-IN-4) is an allosteric inhibitor of the DENV2 and ZIKV NS2B/NS3 proteases, exhibiting IC50 values of 0.69 µM for DENV2 and 1.04 µM for ZIKV NS2B/NS3 proteases [1].

NS2B/NS3-IN-3 (Compd 66) is a Flavivirus NS2B-NS3 protease inhibitor [1].

NS2B/NS3-IN-5 (Compound 25b) is a variant of the DENV2 and ZIKV NS2B/NS3 protease inhibitor with IC50 values of 0.67 μM for ZIKV and 4.38 μM for DENV2.

NS2B/NS3-IN-6 (Compound 1a) is a derivative of the DENV and ZIKV NS2B/NS3 protease inhibitor, exhibiting IC50 values of 2.23 μM for the ZIKV NS2B/NS3 protease and 25.2 μM for the DENV NS2B/NS3 protease.

NS2B/NS3-IN-2 is a potent, covalent inhibitor of dengue virus (DENV) NS2B/NS3 with an IC50 of 6.0 nM and a Ki of 0.66 μM. It significantly increases cell viability and exhibits no cytotoxicity.

Palmatine hydroxide is an orally active, irreversible inhibitor of indoleamine 2,3-dioxygenase 1 (IDO-1), demonstrating IC50 values of 3 μM for HEK 293-hIDO-1 and 157μM for rhIDO-1. Additionally, it uncompetitively inhibits the West Nile virus (WNV) NS2B-NS3 protease with an IC50 of 96 μM. This compound exhibits a range of biological activities, including anti-cancer, anti-oxidation, anti-inflammatory, neuroprotective, antibacterial, and antiviral effects.

NS2B/NS3-IN-7 is a highly potent inhibitor of the Zika virus NS2B-NS3 protease, possessing a Ki value of 2.33 nM. It effectively reduces the number of ZIKV-infected cells.

NS2B/NS3-IN-3 hydrochloride is a Flavivirus NS2B-NS3 protease inhibitor [1].

NS2B/NS3-IN-8, also known as compound MH1, is an inhibitor of the Zika virus NS2B-NS3 protease [1].

Ac-EVKKQR-pNA is a competitive chromogenic substrate targeting the P6-P1 segment at the NS2B-NS3 cleavage site's N-terminal, featuring a highly reactive, hydrolyzable para-nitroaniline (pNA) at the P1 position. This compound holds promise for research into Dengue virus type 2 and Yellow fever virus infections.

DV-B-120 is a competitive inhibitor of the dengue virus (DENV). It inhibits the NS2B-NS3 protease, effectively targeting DENV-1, DENV-2, DENV-3, and DENV-4 with IC50 values of 5.35, 7.39, 10.49, and 8.58 μM, respectively. DV-B-120 exerts its antiviral effects by inhibiting DENV replication.

NSC-323241 is a potent inhibitor of STT3A-mediated mega protein complex assembly. It disrupts the large endoplasmic reticulum (ER) complexes localized by STT3A during dengue virus (DENV) and Zika virus (ZIKV) infections. NSC-323241 interferes with the binding of the STT3A subcomplex to viral non-structural proteins (such as NS2B, NS3) and host transporter proteins, thus impairing the formation of the viral replication microenvironment. This compound holds promise for researching flavivirus infections, including dengue and Zika viruses.

Agathisflavone is a NS2B-NS3 proteases inhibitor of the Dengue virus serotypes 2 and 3.

BP13944 is used as a potential dengue virus NS2B/NS3 protease inhibitor.

5-Hydroxytoluene-2,4-disulphonic acid diammonium, an impurity of Policresulen, is a significant NS2B/NS3 protease inhibitor with an IC50 value of 0.48 μg/mL. It effectively inhibits DENV2 virus replication in BHK-21 cells, with an IC50 of 4.99 μg/mL. Policresulen acts as a competitive inhibitor, marginally impacting the protease's stability[1].

Bz-Nle-Lys-Arg-Arg-AMC is a fluorescent tetrapeptide substrate for the quantitative detection of dengue virus (DV) NS2B-NS3 protease, yellow fever virus (YFV) NS3 protease and Zika virus NS3 protease activities. The rate of release of the cleavage product of the protease, AMC (7-amino-4-methylcoumarin), was observed by hydrolysis reaction to calculate the enzyme activity, with excitation/emission maxima at 340-360/440-460 nm, respectively.

Theaflavin 3,3'-digallate, a major polyphenol found in black tea, is an inducer of oxidative stress which has anti-inflammatory and cancer chemopreventive actions, it reduces tumor angiogenesis by downregulating HIF-1αand VEGF.