Z-Arg-Arg-4MβNA triacetate

Z-Arg-Arg-4MβNA triacetate serves as a specific substrate for cathepsin B, resulting in the generation of the fluorescent end product 4MβNA, which exhibits an excitation wavelength (λ ex) of 355 nm and an emission wavelength (λ em) of 430 nm [1] [2].

Boc-Val-Pro-Arg-MCA TFA is a sensitive fluorescent substrate that is a thrombin substrate for the determination of trypsin-like serine protease activity.

Boc-Gln-Ala-Arg-AMC hydrochloride is a fluorescent compound that serves as a substrate for trypsin and is also used to determine the protein hydrolysis activity of TMPRSS2.

H-Trp-Phe-Tyr-Ser(PO3H2)-Pro-Arg-pNA serves as a chromogenic substrate for Pin1, an essential and conserved mitotic peptidyl-prolyl isomerase that recognizes phosphoserine-proline bonds in mitotic phosphoproteins [1] [2].

CH3OCO-D-CHA-Gly-Arg-pNA acetate serves as a chromogenic substrate for Factor Xa, as documented in sources [1] [2].

Boc-Val-Pro-Arg-MCA hydrochloride (Boc-Val-Pro-Arg-MCA HCl) is a sensitive fluorescent substrate for the determination of trypsin-like serine proteases activity.

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 (FS-6) is a quenched, fluorescent MMP peptide substrate designed for the real-time quantification of MMP enzymatic activity. As an extended variant of the MMP substrate (FS-1), it demonstrates increased specificity against collagenases (MMP-1, MMP-8, MMP-13) and MT1-MMP, evidenced by higher specificity constants (Ex/Em=325 nm/400 nm). [1]

Arg-Val-(Nle-p-nitro)-Phe-Glu-Ala-Nle-NH2 is a fluorogenic substrate for the HIV-1 protease, facilitating the assessment of enzyme activity [1].

Z-Gly-Pro-Arg-4MβNA is a thrombin substrate that, upon cleavage by thrombin, releases 4-methoxy-2-naphthylamine (4MβNA). This liberated 4MβNA reacts with 5-nitrosalicylaldehyde to form an insoluble yellow fluorescent product, indicating the site of thrombin activity [1].

Z-Gly-Pro-Arg-AMC hydrochloride is a fluorescent substrate for both trypsin and cathepsin K, utilized to assay their respective enzymatic activities [1] [2].

Boc-Gln-Ala-Arg-AMC acetate is a fluorogenic substrate primarily used for assaying trypsin activity and additionally suitable for evaluating the proteolytic function of TMPRSS2[1][2].

Z-Ala-Arg-Arg-AMC (Fluorogenic Proteasome Substrate) serves as a fluorogenic substrate designed to assay the trypsin-like activity within the proteasome [1].

Z-(L-Arg)-AMC is a fluorogenic substrate for trypsin, cathepsin B, and cathepsin H.1,2Upon enzymatic cleavage by trypsin, cathepsin B, or cathepsin H, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify trypsin, cathepsin B, and cathepsin H activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively. 1.Zimmerman, M., Ashe, B., Yurewicz, E.C., et al.Sensitive assays for trypsin, elastase, and chymotrypsin using new fluorogenic substratesAnal. Biochem.78(1)47-51(1977) 2.Brindley, P.J., Kalinna, B.H., Dalton, J.P., et al.Proteolytic degradation of host hemoglobin by schistosomesMol. Biochem. Parasitol.89(1)1-9(1997)

H-Arg-4MβNA (H-Arg-4MbNA) is a peptide that serves as a substrate for cathepsin H. The enzyme activity is often detected in gel electrophoresis.

DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS is a fluorescent dye utilized as a fluorogenic substrate for aspartyl proteinase from the human malaria parasite [1]. It requires protection from light during storage.