localization

BODIPY FL prazosin is a fluorescent α1-adrenergic antagonist with binding affinities of Ki: 14.5 nM for α1a-AR and Ki: 43.3 nM for α1b-AR, used to study subcellular localization differences in α1-adrenoceptor subtypes.

Fluorescein O,O′-diacrylate (Diacryloyloxyfluoresin) is a fluorescent compound known for its excellent biocompatibility and cellular labeling capabilities. It is frequently utilized in biological imaging and cell tracking studies to effectively mark cells and monitor their dynamic changes within an organism. Fluorescein O,O′-diacrylate is also applied in compound delivery systems to enhance the precision of compound localization and its inhibitory effects. Additionally, it is used in the preparation of polymer materials to improve their optical properties and biological interactions.

5-Aminofluorescein hydrochloride (5-AF hydrochloride) is a protein fluorescence labeling reagent known for its outstanding fluorescent properties and is extensively utilized in biomedical research for cell imaging and molecular probes. This compound enables the observation of protein localization and dynamic changes, providing insightful biological understanding. Additionally, 5-Aminofluorescein hydrochloride is employed to detect interactions between biomolecules, aiding in the elucidation of complex biological processes.

AF647 C2 maleimide is a water-soluble fluorescent dye that remains stable under a range of physiological conditions. It facilitates the attachment of fluorescent groups to proteins by reacting with thiol groups on target proteins. This labeling method offers high sensitivity and specificity, enabling the detection of proteins at very low concentrations, making it valuable for studying protein expression and localization. Additionally, it can bind to antibodies for labeling cell surface antigens or specific proteins within tissues. [Ex Em(nm)=651 671].

Cyanine7 amine is effective for labeling biomolecules such as proteins, nucleic acids, and cells, facilitating localization, tracking, and detection in various bioanalytical techniques. It provides fluorescent signals for visualization. The excitation emission wavelengths (Ex Em) are 750 773 nm.

DFHBI-1T is an RNA aptamer-activated fluorescent probe that transmits through cell membranes.DFHBI-1T can be used for dynamic localization imaging of RNA in living cells.

Janelia Fluor 585, SE (JF585, SE) is an orange fluorescent dye with an NHS ester that can conjugate with primary amine groups, making it immediately useful for structured illumination (SIM) and stimulated emission depletion (STED) imaging, and convertible to a photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments.

Tetraphenylporphine sulfonate (TPPS) serves as a non-cytotoxic probe suitable for tumor localization detection [1].

Biotin-KKKRKV acetate, the biotinylated form of the nuclear localization signal (NLS) peptide KKKRKV from simian virus 40 [1], serves as a specialized compound for targeted molecular applications.

sCy5DA, a fluorescent D-amino acid, is designed for annotating peptidoglycans in live bacteria, facilitating the imaging of a broad spectrum of bacterial types, including gram-positive, gram-negative, and mycobacteria, with notably efficient incorporation into gram-negative C. crescentus. This compound allows the bacteria it labels to be visualized through single molecule localization microscopy (SMLM; known as PALM or STORM), with excitation emission maxima reported at 646 665 nm.

sCy5DL-amide is a fluorescent D-amino acid designed for peptidoglycan labeling across a broad spectrum of bacteria, encompassing gram-positive, gram-negative, and mycobacteria strains. It demonstrates exceptional incorporation into the Gram-positive B.subtilis. This compound enables the imaging of labeled bacteria using single molecule localization microscopy (SMLM), also known as PALM or STORM, with excitation emission maxima at 646 666 nm.

BODIPY TR methyl ester serves as a lipophilic GFP counterstain, easily permeating cell membranes to accumulate in endomembranous organelles, while exhibiting minimal localization in plasma membranes. It is a highly effective red fluorescent vital dye (Ex=568 nm, Em=625 nm) that can reveal the location and shape of cell nuclei, the morphology of cells in embryonic tissues, and the boundaries of organ-forming tissues within an entire embryo [1].

C6 NBD galactosylceramide, an active derivative of galactosylceramide tagged with fluorescent nitrobenzoxadiazole (NBD), serves as a substrate for neutral β-glycosylceramidase (GCase) in studies of intracellular localization and metabolism of galactosylceramide (Ex=nm, Em=525) [1].

CY5.5-COOH (Cyanine 5.5 carboxylic acid) is a fluorescent dye commonly used in biological imaging, cell imaging, protein localization, and immunostaining.

Kdn probe-1, a fluorescent Kdn probe, identifies the localization of [AfS] within vesicles on the cell surface.

5-TRITC is an amine-reactive fluorescent dye commonly used to label proteins and nucleic acids. It reacts with amino groups in proteins to form stable thioether bonds, thereby labeling and detecting the presence and localization of specific proteins, producing bright red-orange fluorescence with excitation emission maxima of about 555 580 nm.