flow cytometry

7-Amino-4-methylcoumarin-3-acetic acid [7-Amino-4-methylcoumarin] is a more suitable substrate for enteropeptidase compared to GD(4)K-NA.

GLF16 HCl is a fluorophore-coupled Sudan Black B analog that allows rapid detection, isolation and real-time tracking of senescent cells by fluorescence microscopy and flow cytometry.

CDy9 is a highly selective fluorescent probe for the detection and isolation of mESC using fluorescence microscopy and flow cytometry. CDy9 allows the detection and isolation of intact stem cells with marginal impact on their function and capabilities. CD

BODIPY-aminoacetaldehyde diethyl acetal (BAAA-DA) is a stable precursor to BODIPY-aminoacetaldehyde, a cell-permeable fluorescent substrate for aldehyde dehydrogenase (ALDH).1,2BODIPY-aminoacetaldehyde diethyl acetal is converted under acidic conditions to BODIPY-aminoacetaldehyde (BAAA).2BAAA is cell-permeant and is converted intracellularly by ALDH to BODIPY aminoacetate (BAA), which is retained by cells and can be used to identify cells with high ALDH activity.1BAA is a substrate for the efflux pump P-glycoprotein (P-gp) but co-application of BAAA with a P-gp inhibitor, such as verapamil , inhibits BAA efflux.2BAAA-DA has been used to isolate human hematopoietic progenitor cells, which have high ALDH activity, andviaflow cytometry to sort cancer stem cells that contain high levels of ALDH.1,3BAA used in cells can be excited at 488 nm and displays an emission maximum of 512 nm.4 1.Storms, R.W., Trujillo, A.P., Springer, J.B., et al.Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activityProceedings of the National Academy of Sciences of the United States of America96(16)9118-9123(1999) 2.Smith, C.A., Colvin, M., Storms, R.W., et al.BODIPY aminoacetaldehyde diethyl acetal08010501.81-15(2010) 3.Leng, Z., Yang, Z., Li, L., et al.A reliable method for the sorting and identification of ALDHhigh cancer stem cells by flow cytometryExp. Ther. Med.(2017) 4.Pomper, M.G., Wang, H., Minn, I., et al.Red fluorescent aldehyde dehydrogenase (ALDH) substrate(2015)

DiIC1(5) is a signal-off fluorescent probe for the detection of mitochondrial membrane potential disruption. It accumulates in mitochondria and its fluorescence intensity decreases when the mitochondrial membrane potential is disrupted. DilC1(5) has been used in combination with a variety of cell damage and cell death markers to classify nine stages of cell death using flow cytometry. It has also been used as a quencher for the detection of serine phosphorylation and tyrosine dephosphorylation post-translational modifications (PTMs) in quenching resonance energy transfer (QRET) and mTR-FRET applications. It displays excitation/emission maxima of 659/666 nm, respectively.

Fluorescein tyramide is a widely utilized green fluorescent reagent (λ abs : 494 nm; λ em : 517 nm), primarily employed for tyramide signal amplification (TSA) in low-abundance IHC, ICC, in situ hybridization (FISH), and flow cytometry (FCM) applications.

FITC-labeled ODN 1018 (sodium), a TLR-9 agonist oligodeoxynucleotide, enables the evaluation of CpG ODN cellular uptake and localization via confocal laser-scanning microscopy [excitation 495 nm, emission 520 nm] or flow cytometry.

FITC-labeled Agatolimod (sodium), a class B CpG ODN (oligodeoxynucleotide) and TLR9 agonist, enables the evaluation of CpG ODN cellular uptake and localization using confocal laser-scanning microscopy (excitation 495 nm, emission 520 nm) or flow cytometry.

FITC-labeled ODN 1826 (sodium), a class B CpG ODN (oligodeoxynucleotide) and TLR9 agonist, enables the evaluation of CpG ODN cellular uptake and localization via confocal laser-scanning microscopy (excitation 495 nm, emission 520 nm) or flow cytometry.

FITC-labeled ODN 1585 (sodium) effectively induces IFN and TNFα production and serves to assess CpG ODN cellular uptake and localization via confocal laser-scanning microscopy [excitation 495 nm, emission 520 nm] or flow cytometry.

FITC-labeled ODN 1668 (sodium), a class B CpG oligodeoxynucleotide (ODN), functions as a TLR9 agonist and is used to assess the cellular uptake and localization of CpG ODNs via confocal laser-scanning microscopy (excitation 495 nm, emission 520 nm) and flow cytometry.

FITC-labeled ODN 2088 (sodium) is a potent inhibitor of TLR3, TLR7, and TLR9, used to assess CpG ODN cellular uptake and localization via confocal laser-scanning microscopy (excitation 495 nm, emission 520 nm) or flow cytometry.

FITC-labeled ODN 2216 (sodium), a specific agonist for human toll-like receptor 9 (TLR9), facilitates the assessment of CpG ODN cellular uptake and localization through confocal laser-scanning microscopy (excitation 495 nm, emission 520 nm) or flow cytometry.

FITC-labeled ODN 2395 (sodium), a class C oligodeoxynucleotide and TLR9 agonist, facilitates the assessment of CpG ODN cellular uptake and localization via confocal laser-scanning microscopy [excitation 495 nm, emission 520 nm] or flow cytometry.

FITC-labeled ODN TTAGGG (sodium) is an inhibitory oligonucleotide that antagonizes TLR9, AIM2, and cGAS, aiding in assessing CpG ODN cellular uptake and localization through confocal laser-scanning microscopy (excitation at 495 nm, emission at 520 nm) or flow cytometry.

Fluorescein-6-carbonyl-Asp(OMe)-Glu(OMe)-Val-DL-Asp(OMe)-fluoromethylketone is a cell-permeable, non-toxic inhibitor that irreversibly binds to activated caspase-3 in apoptotic cells, with fluorescence intensity measurable via flow cytometry, microwell plate reader, or fluorescence microscopy [1].

eGFP mRNA-LNP is a lipid nanoparticle encapsulating eGFP mRNA, designed to evaluate RNA delivery, translation efficiency, and cell viability. Upon cell internalization, it expresses Enhanced Green Fluorescent Protein (eGFP), enabling detection through fluorescence microscopy or flow cytometry [1].

eGFP circRNA-LNP, a lipid nanoparticle comprising eGFP circRNA, facilitates studies of RNA delivery, translation efficiency, and cell viability. This compound includes Enhanced Green Fluorescent Protein (eGFP) for expression within cells, detectable via fluorescence microscopy or flow cytometry [1].

CELT-327 is a potent, selective fluorescent hA2B/A3 adenosine receptor antagonist, exhibiting K_i values of 35.6 nM at A2B and 45.7 nM at A3 receptors. It efficiently labels HCT116 cells expressing A2B adenosine receptors in a dose-dependent manner, demonstrating rapid, uniform spread and integration of fluorescence within cell monolayers. In live HCT116 spheroids, it achieves consistent staining throughout, penetrating even the deepest layers. As a fluorescent adenosine receptor probe, CELT-327 is ideal for applications in fluorescence binding assays, live cell imaging, flow cytometry, and fluorescence polarization assays, serving as an effective alternative to GPCR radiolabeling ligands. Its excitation and emission peaks are at 589 nm and 616 nm, respectively, making it suitable for TR-FRET assays as an acceptor dye, particularly in combination with the CoraFlor1 TR-FRET donor.

CELT-426 is a potent, partially selective fluorescent antagonist of the human D2 dopamine receptor, exhibiting Ki values of 89.3 nM, 194.8 nM, and 263.6 nM for D2, D3, and D4 dopamine receptors, respectively, in radioligand binding assays. This compound serves as an effective fluorescent probe for dopamine receptors, facilitating its application in a range of techniques including fluorescence binding assays, live cell imaging, flow cytometry, and fluorescence polarization assays. It provides an alternative to GPCR radiolabeling ligands. CELT-426 possesses excitation and emission maxima (λ) of 560 nm and 571 nm, respectively, making it suitable for use as an acceptor dye in Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) assays in conjunction with the CoraFluor1 TR-FRET donor.

FAM-labeled ODN 1826 sodium is a class B CpG ODN and acts as a TLR9 agonist. The cellular uptake and localization of CpG ODNs by FAM-labeled ODN 1826 sodium can be assessed using confocal laser scanning microscopy or flow cytometry.

DSPE-PEG-Cy3, MW 2000 is a PEG lipid conjugated with a fluorescent group. The Cy3 fluorophore is commonly utilized in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. This dye exhibits peak absorption at wavelengths between 548-552 nm and maximum emission between 562-570 nm.

DSPE-PEG-Cy3, MW 3400, is a PEG lipid conjugated with a fluorescent group. The Cy3 fluorophore is frequently utilized in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. This dye exhibits a peak absorption wavelength in the range of 548-552 nm and a peak emission wavelength between 562-570 nm.

DSPE-PEG-Cy3, MW 5000, is a PEG lipid conjugated with a fluorescent group. The Cy3 fluorophore is commonly utilized in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. This dye exhibits peak absorption at wavelengths between 548-552 nm and peak emission between 562-570 nm.