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Conjugation and labeling

Azide

Azide dyes are a class of chemical reagents containing an azide group (-N₃) that can react efficiently and specifically with alkynyl or cyclooctyne groups through click chemistry reactions. These dyes are widely used in biomolecule labeling, live cell imaging, and materials science. The reaction conditions are mild, do not affect the activity of biological systems, and have the advantages of high selectivity and low background interference.

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Cy5-N3

T189421621101-43-6

CY5-N3 (Sulfo-Cyanine5-azide) is an anthocyanin fluorescent dye with azide groups, utilized for cell imaging, tissue imaging, and in vivo imaging.

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C.I. Food Yellow 10

T3066785-84-7

C.I. Food Yellow 10, also known as Fast Yellow AB or Acid Yellow, is an azo dye used primarily as a food dye that is now delisted in both Europe and USA and is forbidden if used in foods and drinks. Ungraded products supplied by Spectrum are indicative of

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DIBA-Cy5

T74988

DIBA-Cy5, a fluorescent DIBA antagonist, consists of DIBA-alkyne and Cyanine5 fluorophores (Cy5) linked by a polyethylene glycol (PEG) chain. It functions as a fluorescent ligand for probe attachment via click chemistry, exhibiting high binding affinity to type-2 mAChR (M2R) with a dissociation constant (Kd) of 1.80 nM. Notably, DIBA-Cy5 effectively stains M2R receptors in the sinoatrial node of mouse hearts [1].

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DBCO-Cy3

T189691782950-79-1

DBCO-Cy3 (DBCO-Sulfo-Cy3) is a derivative of the Cyanine3 fluorophore, an orange fluorescent dye that is pH insensitive from pH 4-10, with an excitation maximum of 555 nm and an emission maximum of 580 nm.