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Conjugated and labeled dyes are a class of chemical substances widely used in biomolecule labeling and detection. They achieve stable binding with target molecules through specific functional groups such as amine, azide, thiol, etc., thereby giving the target molecules traceability or visualization properties. This type of dye covers a variety of types, including Janelia Fluor series, BODIPY series, biotin, coumarins, cyanines, fluoresceins, rhodamines, and bioconjugated azofluoresceins. Each type of dye is used in different biological applications due to its unique optical properties and chemical stability, such as cell imaging, flow cytometry, immunohistochemistry, etc. These dyes not only improve the detection sensitivity and specificity of biomolecules, but also expand the understanding of the dynamic changes of life processes, which is crucial for modern biological research, especially in the fields of molecular biology and cell biology.
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