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Conjugation and labelingJanelia Fluor Series
Janelia Fluor series dyes are a class of high-brightness, excellent photostability fluorescent dyes designed for super-resolution microscopy and single-molecule detection. Their unique chemical structure enables them to maintain a high signal-to-noise ratio under multiple laser excitations, making them suitable for live cell imaging, immunofluorescence labeling, and biomolecule tracking. Compared with traditional fluorescent dyes, they have lower photobleaching rates and higher imaging accuracy.
Janelia Fluor® 646, SE
T392421811539-59-9
Janelia Fluor 646, SE (JF646, SE) is a red fluorescent dye used in cellular imaging.
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Super Fluor 680, SE
TD0028
This Super Fluor 680, SE is a reactive dye for the labeling of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.
- $336
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Janelia Fluor® 549, Azide
T82015
Janelia Fluor® 549, Azide (JF549, Azide) is a fluorescent dye with an absorption maximum (λab (max)) of 549 nm and an emission maximum (λem (max)) of 571 nm.
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Janelia Fluor® 549, Tetrazine
T82013
Janelia Fluor® 549, Tetrazine (JF549, Tetrazine) is a fluorescent dye with an absorption maximum (λab (max)) of 549 nm and an emission maximum (λem (max)) of 571 nm [1].
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Janelia Fluor® 646, Azide
T78395
Janelia Fluor 646, Azide (JF646, Azide) is a red-emitting fluorogenic dye with an Azide click chemistry group, ideal for live-cell imaging experiments [1][2].
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Janelia Fluor® 646, Tetrazine
T784112042192-00-5
Janelia Fluor 646, Tetrazine (JF646, Tetrazine) is a red fluorescent dye with a tetrazine moiety, suitable for cellular imaging [1].
- $1,430
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Janelia Fluor® 525, SE
T78394
Janelia Fluor 525, SE (JF525, SE) is a yellow fluorescent dye with an excitation wavelength of 525 nm and an emission wavelength of 549 nm [1].
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Janelia Fluor®646b, Maleimide
T879622634129-70-5
Janelia Fluor®646b, Maleimide, is a blinking red fluorescent dye equipped with a maleimide group that allows for conjugation through thiol reactivity.
- $1,970
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Janelia Fluor® 549, Maleimide TFA
T82014
Janelia Fluor® 549, Maleimide TFA (JF549, Maleimide TFA) is a fluorescent dye with an absorption maximum (λab (max)) at 549 nm and an emission maximum (λem (max)) at 571 nm [1].
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JFX 650, NHS ester
T2013792396479-84-6
JFX 650, NHS ester, a deuterated red fluorescent Janelia Fluor® dye, is provided with an NHS ester reactive group for labeling primary amines. It is ideal for live cell imaging.
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Janelia Fluor 525, Haloalkan
T2013801811539-37-3
Janelia Fluor 525, Haloalkane is a yellow, cell-permeable, fluorogenic fluorescent dye featuring a chloroalkane handle that exhibits a high degree of fluorogenicity. The excitation and emission maxima (λ) of this compound are 525 nm and 549 nm, respectively, with a quantum yield of 0.91.
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PA Janelia Fluor 646, SE
T395042093120-32-0
PA Janelia Fluor 646, SE (PA-JF646-NHS), a photoactivatable fluorescent dye, is an NHS ester for coupling to primary amine groups. PA-JF646-NHS is non-fluorescent until activated at 365 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g.HaloTag and SNAP-tag . PA-JF646-NHS is used for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. (λ Excitation/emission ~650/664 nm).
- $638
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Janelia Fluor 549, Haloalkane
T2013811811539-33-9
Janelia Fluor 549, Haloalkane, a yellow cell-permeable fluorogenic fluorescent dye featuring a chloroalkane handle, exhibits a high level of fluorogenicity. The compound's excitation and emission maxima (λ) are respectively 549 nm and 571 nm, with a quantum yield = 0.88.
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Janelia Fluor 585, Haloalkane
T2013821811539-90-8
Janelia Fluor 585, Haloalkane, is an orange cell-permeable fluorogenic fluorescent dye featuring a chloroalkane handle. This compound exhibits a high level of fluorogenicity. It has excitation and emission maxima (λ) at 585 nm and 609 nm, respectively, with a quantum yield of 0.78.
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Janelia Fluor® 669, SE
T395752127150-20-1
Janelia Fluor 669, SE (JF669, SE), a red fluorescent dye, reacts directly with thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to produce a JF669 HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm).
- $1,620
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Janelia Fluor® 549, SE
T392401811539-32-8
Janelia Fluor 549, SE (JF549, SE) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm.
- $1,430
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PA Janelia Fluor® 549, SE
T392411811539-42-0
PA Janelia Fluor 549, SE (PA-JF549-NHS) is a bright photoactivatable fluorophore of JF549, SE (JF549, NHS). JF549, SE (JF549, NHS) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm.
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Janelia Fluor 635, Haloalkane
T2013831811539-66-8
Janelia Fluor 635, Haloalkane, a red cell-permeable fluorogenic fluorescent dye featuring a chloroalkane handle, exhibits significant fluorogenicity. The excitation and emission maxima (λ) for this compound are 635 nm and 652 nm, respectively, with a quantum yield of 0.56.
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Janelia Fluor® 646 TFA
T78396
Janelia Fluor® 646 TFA (JF646 TFA) is a red fluorogenic dye used in the synthesis of HaloTag and SNAP-Tag ligands for Janelia Fluor 646, and in live cell imaging applications [1] [2].
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Janelia Fluor® 585, SE
T392431811539-88-4
Janelia Fluor 585, SE (JF585, SE) is an orange fluorescent dye with an NHS ester that can conjugate with primary amine groups, making it immediately useful for structured illumination (SIM) and stimulated emission depletion (STED) imaging, and convertible to a photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments.
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Janelia Fluor® 526, SE
T82016
Janelia Fluor 526, SE (JF526, SE) is a fluorogenic yellow fluorescent dye with an NHS ester group, serving as a versatile scaffold for creating fluorogenic ligands, suitable for labeling genetically encoded self-labeling protein tags and staining endogenous structures [1].
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Janelia Fluor® 549 TFA
T397592245946-45-4
Janelia Fluor 549 TFA (JF549 TFA) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm.
- $553
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Janelia Fluor® 646, Maleimide
T78397
Janelia Fluor® 646, Maleimide (JF646, Maleimide), is a red fluorescent dye containing a maleimide group, suitable for cellular imaging [1] [2].
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Janelia Fluor 646, Haloalkane
T2013841811539-60-2
Janelia Fluor 646, Haloalkane, a red cell-permeable fluorogenic fluorescent dye featuring a chloroalkane handle, exhibits significant fluorogenicity. The excitation and emission maxima (λ) for this compound are 646 nm and 664 nm, respectively, with a quantum yield of 0.54.
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