protein purification

Edetic acid (Ethylenediaminetetraacetic acid) is a chelating agent commonly used in protein purification, both to eliminate contaminating divalent cations and to inhibit protease activity.

MES monohydrate is an amphoteric ionic buffer that is a PH stabilizing reagent used for protein purification and enzyme assays.

MOPSO is an amphoteric ionic biological buffer with an effective pH range of 6.2 to 7.6, commonly used in cell culture, enzyme activity assays, and protein purification experiments.

Lauroylsarcosine sodium salt(Sarkosyl NL sodium) is an anionic surfactant and detergent with protein denaturing properties commonly used in molecular biology research for purification of membrane proteins, promotion of cell lysis in RNA purification experiments and membrane solubilization, and toxicity to A549 cells after ALIF or SME exposure.

Potassium acetate (Diuretic salt) with antibacterial and antifungal properties.

MOPSO sodium is an amphoteric ionic biological buffer with an effective pH range of 6.2 to 7.6, commonly used in cell culture, enzyme activity assays, and protein purification experiments.

Gel filtration medium G-100, a substance designed for protein purification, enables the separation and purification of proteins through gel filtration.

ZWITTERGENT 3-16 Detergent is a quaternary ammonium surfactant, emulsifier, foaming and wetting agent commonly used in the skin care industry.ZWITTERGENT 3-16 Detergent is involved in protein purification.

Cross-linked dextran G 200 is a hydrophilic gel designed for the separation of macromolecules based on molecular size exclusion. It operates through a gel permeation mechanism; its cross-linked structure forms a three-dimensional network with specific pore sizes, facilitating separation according to the hydrodynamic volume of molecules. This compound is utilized to regulate the partition coefficient of permeating solutes and maintain osmotic balance, and is commonly applied in gel filtration chromatography for the purification and analysis of biomacromolecules like proteins and nucleic acids. Additionally, Cross-linked dextran G 200 serves as a gel filtration medium (particle size range: 40-120 μm; spherical protein separation range: 5k-600k Da).

Ethylenediaminetetraacetic acid (EDTA) sodium hydrate is a metal chelator that binds with divalent and trivalent metal cations, including calcium. It exhibits antibacterial, anti-inflammatory, antioxidant, anti-hypercalcemic, and anticoagulant properties. EDTA sodium hydrate reduces protein oxidation damage catalyzed by metal ions and maintains a reducing environment during protein purification, making it a common choice for protein purification and storage. Additionally, it can alleviate liver fibrosis and is used in research related to coronary artery disease and neurological disorders.

Sterile 10 mM Histidine, pH 5.5 buffer is a versatile solution with significant applications in protein purification, vaccine preparation, drug development, cell culture, and electrophoresis. Histidine, an amino acid, provides excellent buffering capacity. Its sterility and specific pH conditions make it ideal for experiments requiring precise environmental control. Histidine in this buffer can derive photosensitizers, which increase significantly upon light exposure. These histidine-derived photosensitizers act as primary mediators in the photooxidation of tryptophan.