protein purification

Edetic acid (Ethylenediaminetetraacetic acid) is a chelating agent commonly used in protein purification, both to eliminate contaminating divalent cations and to inhibit protease activity.

3X FLAG peptide consists of three repeating Asp-Tyr-Lys-Xaa-Xaa-Asp motifs with a FLAG tag at the C-terminus of the peptide.3X FLAG peptide can be used for protein identification and purification.

N-Dodecyl-β-D-maltoside (Lauryl Maltoside) is an alkyl maltopyranoside detergent, particularly useful in transporters and respiratory complexes. It is a derivative of pyrene (Py) and has applications in the purification and stabilization of RNA polymerase, as well as the detection of protein-lipid interactions.

EDTA copper(II) disodium salt is a metal chelator with antimicrobial, anti-inflammatory and antioxidant activity, slows down damage caused by metal ions, maintains osmotic pressure during protein purification, and can be used in the study of neurological disorders.

MES monohydrate is an amphoteric ionic buffer that is a PH stabilizing reagent used for protein purification and enzyme assays.

MOPSO is an amphoteric ionic biological buffer with an effective pH range of 6.2 to 7.6, commonly used in cell culture, enzyme activity assays, and protein purification experiments.

Lauroylsarcosine sodium salt(Sarkosyl NL sodium) is an anionic surfactant and detergent with protein denaturing properties commonly used in molecular biology research for purification of membrane proteins, promotion of cell lysis in RNA purification experiments and membrane solubilization, and toxicity to A549 cells after ALIF or SME exposure.

Potassium acetate (Diuretic salt) with antibacterial and antifungal properties.

MOPSO sodium is an amphoteric ionic biological buffer with an effective pH range of 6.2 to 7.6, commonly used in cell culture, enzyme activity assays, and protein purification experiments.

Nonaethylene glycol monododecyl ether (Polidocanol) is a nonionic surfactant and polyethylene glycol (PEG) detergent. It can be used to form initial coalesced O/W emulsion droplets and for protein separation and purification.

EDTA tetrasodium tetrahydrate is a metal chelator known for its ability to bind with divalent and trivalent metal cations, including calcium. It exhibits antihypercalcemic and anticoagulant properties. This compound reduces metal ion-catalyzed oxidative damage to proteins and maintains a reducing environment during protein purification processes, making it commonly used for protein purification and storage. Additionally, it helps in minimizing the formation of disulfide bonds.

Phospho-Glycogen Synthase Peptide-2, a substrate for glycogen synthase kinase-3 (GSK-3), serves as a peptide substrate effective in the affinity purification of protein-serine kinases.

Gel filtration medium G-100, a substance designed for protein purification, enables the separation and purification of proteins through gel filtration.

ZWITTERGENT 3-16 Detergent is a quaternary ammonium surfactant, emulsifier, foaming and wetting agent commonly used in the skin care industry.ZWITTERGENT 3-16 Detergent is involved in protein purification.

HA Peptide TFA is a nine–amino acid synthetic peptide derived from human influenza hemagglutinin (HA) that is widely applied in cell biology and biochemistry research for the isolation, purification, detection, and tracking of target proteins, serving as a versatile epitope tag and experimental reagent in protein expression and interaction studies.

Phospho-Glycogen Synthase Peptide-2 (substrate) serves as a peptide substrate for glycogen synthase kinase-3 (GSK-3) and is used for the affinity purification of protein-serine kinases [1].

Ac-MBP (4-14) Peptide, an acetylated form of the MBP (4-14) peptide, serves as a highly selective substrate for (protein kinase C) PKC. It facilitates PKC assays in extracts without the need for prior purification to remove interfering protein kinases or phosphatases [1].

3x FLAG-DYKDDDDK Tag is a versatile tool utilized for studying protein function and localization when specific antibodies (Abs) are unavailable. It is frequently employed in its 3x FLAG format to facilitate the purification of proteins present in low quantities [1] [2].

Streptavidin is a protein tetramer purified from bacteria with a very high affinity for biotin and is commonly used for the purification or detection of various biomolecules. Streptavidin is immunologically active, inhibiting IL-2 synthesis and CD25 expression in T cells.

Cross-linked dextran G 200 is a hydrophilic gel designed for the separation of macromolecules based on molecular size exclusion. It operates through a gel permeation mechanism; its cross-linked structure forms a three-dimensional network with specific pore sizes, facilitating separation according to the hydrodynamic volume of molecules. This compound is utilized to regulate the partition coefficient of permeating solutes and maintain osmotic balance, and is commonly applied in gel filtration chromatography for the purification and analysis of biomacromolecules like proteins and nucleic acids. Additionally, Cross-linked dextran G 200 serves as a gel filtration medium (particle size range: 40-120 μm; spherical protein separation range: 5k-600k Da).

AC Antibody Purification Resin 2 is composed of spherical, highly cross-linked agarose beads characterized by a narrow size distribution and strong mechanical stability. It is utilized for the separation and purification of complex antibodies such as monoclonal, bispecific, and polyclonal antibodies as well as Fc fusion proteins. [Substrate: Highly cross-linked agarose microspheres; Particle size: 65μm; Ligand: Alkali-resistant recombinant Protein A; ADC Purification Resin.]

AC Antibody Purification Resin 1 is a 69μm nanometer-sized agarose designed for ADC purification. It contains the ligand: recombinant protein A.

AC mAb-X purification resin is a specialized Protein A resin. It features a highly rigid agarose matrix and particle sizes ranging from 40 to 120 μm. The ligand is a recombinant alkaline-resistant Protein A (~24KD), and it serves as a purification resin for ADC.

Ethylenediaminetetraacetic acid-D12 is the deuterated form of Ethylenediaminetetraacetic acid. Ethylenediaminetetraacetic acid (T20470) (EDTA) serves as a metal chelator, binding with divalent and trivalent metal cations, including calcium. It exhibits antihypercalcemic and anticoagulant properties, reduces metal ion-catalyzed oxidative damage to proteins, and maintains a reducing environment during protein purification. EDTA is commonly used in protein purification and storage and helps minimize the formation of disulfide bonds.