primary

AQC (6-Aminoquinolyl-N-hydroxysccinimidyl carbamate) is a reagent used in HPLC for the fluorescence detection of amino acid or protein sequences. It reacts with primary and secondary amino acids to produce fluorescent derivatives, enabling the detection of amino acids at picomolar levels.

Alexa fluor 647 NHS ester (L-5-Hydroxytryptophan-d3) is a photostable far-red fluorescent dye used in imaging and flow cytometry to generate stable signals for protein labeling, amine-modified oligonucleotides, and other primary amine-containing molecules.

5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.

6-Carboxyfluorescein (6-FAM) contains a carboxylic acid functional group, facilitating reactions with primary amines through carbodiimide activation.

5-FAM (5-Carboxyfluorescein) (5-Carboxyfluorescein) contains a carboxylic acid, which can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its excellent fluorescence quantum yield, relatively high absorptivity, and good water solubility, it also has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.

5(6)-FAM SE (5(6)-Carboxyfluorescein N-hydroxysuccinimide ester) is an amine-reactive green fluorescent dye. 5(6)-FAM SE is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

5(6)-TAMRA (5(6)-Carboxytetramethylrhodamine) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. It is bright, orange-fluorescent dye produces conjugates with absorption emission maxima of ~555 580 nm.

Dansyl chloride (DNSC-l) reacts with primary amino groups in both aliphatic and aromatic amines to produce stable blue- or blue-green-fluorescent sulfonamide adducts. It is widely used to modify amino acids, specifically, protein sequencing and amino acid analysis.

Fluorescamine (Ro 20-7234) ( Ro 20-7234) is not fluorescent itself but reacts with primary amines to form highly fluorescent products. It is used to detect amines and peptides.

JFX 650, NHS ester, a deuterated red fluorescent Janelia Fluor® dye, is provided with an NHS ester reactive group for labeling primary amines. It is ideal for live cell imaging.

NUN 89065, also known as N-(Azido-PEG2)-N-Fluorescein-PEG4-acid, is a fluorescein dye derivative featuring terminal azido and carboxylic acid groups. The azido group facilitates PEGylation through click chemistry, while the carboxylic acid group can react with primary amines in the presence of activators (e.g., EDC or DCC) to form stable amide bonds. Currently, this product lacks an official name, and thus, this temporary designation is used according to MedKoo's chemical naming rules [see website: https: www.medkoo.com page naming].

Alexa Fluor 430 NHS ester is a compound used to conjugate the green fluorescent dye Alexa Fluor 430 with proteins or antibodies. Alexa Fluor 430 provides stable signal generation in imaging and flow cytometry. The NHS ester is suitable for labeling proteins, amine-modified oligonucleotides, and other primary amine-containing molecules (R-NH2).

OVA-PEG-Cy3 is a conjugate of OVA-PEG labeled with Cy3. The Cy3 fluorophore is widely used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry, with its maximum emission wavelength approximately at 562-570 nm. Ovalbumins (OVA) are the primary proteins found in egg whites, known for their diverse biological activities, including anticancer, antihypertensive, antibacterial, antioxidant, and immunoregulatory effects. Ovalbumins are the most abundant proteins synthesized in oviducts treated with progesterone or estrogen and are commonly used as markers to study hormone regulation of gene expression in tissues.

Sulfo ICG bis-NHS ester is a derivative of Indocyanine green (ICG) that contains sulfonic acid ions. This compound is employed in the labeling of proteins, amine-modified oligonucleotides, and other primary amine (R-NH2) containing molecules.

BP Fluor 350 NHS ester is an amine-reactive, water-soluble dye that emits blue. It is specifically designed to efficiently modify primary amines at pH 7-9, resulting in the formation of a stable covalent amide bond.

Sulfo Cy7 bis-NHS ester is a derivative of a Cy7 dye containing sulfonate ions. It can be used to label proteins, amine-modified oligonucleotides, and other primary amine-containing (R-NH2) molecules.

Sulfo Cy3 bis NHS ester is a derivative of Cy3 dye containing sulfonate ions. It is utilized for labeling proteins, amine-modified oligonucleotides, and other primary amine (R-NH2) containing molecules.

BP Fluor 568 NHS ester is an orange fluorescent dye that reacts with amines. It is commonly used for labeling proteins, antibodies, and oligonucleotides through primary amino groups (lysine), amine modifications, and other amino groups [excitation emission wavelengths: 578 nm 602 nm].

IRDye 680RD, NHS ester, is a near-infrared (NIR) fluorescent dye equipped with an NHS ester reactive group, allowing it to label primary amines.

AF647 NHS is a fluorescent dye commonly used for biomolecule labeling. It exhibits far-red fluorescence with Ex Em(nm)= 648nm 671nm. The active ester group of this dye reacts with amino groups (mainly primary amines) on target biomolecules, forming stable amide bonds, thus labeling AF647 fluorescent molecules onto biopolymers.

BODIPY 493 503 NHS ester is a widely used reagent for conjugating this dye to proteins or antibodies. The NHS ester reacts with primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting BODIPY 493 503 conjugates exhibit bright fluorescence, narrow emission bandwidth, and relatively long excited-state lifetimes, which are suitable for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Ex Em(nm) = 496 508.

Cyanine5.5 NHS Ester (ethyl) is a far-red fluorescent dye with an N-hydroxysuccinimide (NHS) active group that can react with primary amines (-NH₂) in proteins, peptides or other biological molecules to achieve specific labeling. It is widely used in the fields of biological imaging and fluorescence analysis.

TAMRA-PEG4-NHS ester is a TAMRA red fluorescent dye derivative containing an NHS ester group which can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Gallocyanine is a small molecule blue dye used to stain nuclei. It is known to inhibit Dickkopf-1 (Dkk1), an antagonist of the Wnt pathway. Gallocyanine can disrupt the interaction of Dkk1 with its receptor LRP5 6 (IC50 = 3 μM), thereby activating Wnt β-catenin signaling. Modulation of the Dkk1-LRP5 6 interaction via gallocyanine has been shown to inhibit prostaglandin J2-induced tau phosphorylation at serine396 in primary cortical neurons in vitro.