click 3

Tris(benzyltriazolylmethyl)amine (TBTA) is a tertiary amine with three 1,2,3-triazole groups that complexes with and stabilizes copper(I), thereby accelerating azide-alkyne cycloadditions used in click chemistry.

3-Azidopropionic Acid Sulfo-NHS Ester is a water-soluble compound containing an azide group and an NHS ester. The azide group can react with alkyne, BCN, DBCO via Click Chemistry to yield a stable triazole linkage. The NHS ester can be used to label the p

Smoothened (SMO) is a GPCR-like receptor which, with Patched, mediates hedgehog signaling to regulate gene expression through the Gli transcription factors. 20(S)-hydroxy Cholesterol (20(S)-OHC) is an oxysterol which binds SMO and activates hedgehog signaling (EC50 = 3 μM), and this activation is selective for the nat-20(S)-OHC enantiomer. Nat-20(S)-OHC synergizes with the SMO agonist SAG, suggesting an allosteric effect. Nat-20(S)-yne is a form of nat-20(S)-OHC with a terminal alkyne group, which can be used in linking reactions known as click chemistry. Click chemistry involves highly dependable and specific azide-alkyne bioconjugation reactions and can be used to capture or immobilize bioactive molecules. Thus, nat-20(S)-yne has been conjugated with magnetic beads to demonstrate that nat-20(S)-OHC directly binds SMO.

Suberoylanilide hydroxamic acid (SAHA) is a class I and class II histone deacetylase (HDAC) inhibitor that binds directly to the catalytic site of the enzyme thereby blocking substrate access. SAHA-BPyne is a SAHA derivative with a benzophenone crosslinker and an alkyne tag intended to be used for profiling HDAC activities in proteomes and live cells. Such terminal alkyne groups can be used in linking reactions, known as click chemistry, characterized by high dependability and specificity of azide-alkyne bioconjugation reactions. SAHA-BPyne labels HDAC complex proteins both in proteomes at 100 nM and in live cells at 500 nM and demonstrates an IC50 value of ~3 μM for inhibition of HDAC activity in HeLa cell nuclear lysates in an HDAC activity assay.

Mild oxidation can convert the sulfhydryl group of cysteine residues on proteins to cysteine-sulfenic acid derivatives (Cys-SOH). Protein sulfenylation, then, is a post-translational modification that is relevant to redox signaling. DYn-2 is a chemoselective probe for detecting sulfenylated proteins in intact cells. DYn-2 consists of 1,3-cyclohexanedione coupled to an alkyne moiety by a 3-carbon spacer. The cyclohexanedione group selectively reacts with protein sulfenic acid modifications. The alkyne group of DYn-2 can then be detected using azide-bearing tags by standard click chemistry. This approach offers superior sensitivity relative to using azide-modified probes with alkynyl detection tags.

Biotin-azide (N-(3-Azidopropyl)biotinamide) is used for preparing various biotin affixes and identifying proteins.

3-Azido-D-alanine hydrochloride, an azido-modified derivative of D-alanine hydrochloride, serves as a reagent in click chemistry labeling research [1].

5'-Ethynyl-2'-deoxycytidine (EdC) functions as a nucleoside analog effectively inhibiting the replication of herpes simplex virus-1 (HSV-1) KOS strain with an ID50 of 0.2 μg/mL. Additionally, it decreases virus-induced cytopathogenic effects in PRK cells for HSV-1, HSV-2, and vaccinia virus strains, demonstrating MIC values between 0.2 to 5 μg/mL. EdC acts as a thymidylate synthetase inhibitor, selectively impacting the DNA incorporation rates of [1',2'-3H]deoxyuridine compared to [CH3-3H]deoxythymidine in PRK cells with ID50s of 3 and 120 μg/mL, respectively. It further inhibits thymidine synthetase and curtails L1210 cell proliferation, a process reversible by deoxythymidine (ID50s are 4.4 and 1,000 μg/mL, respectively). Also, EdC is utilized in monitoring DNA synthesis and cellular replication through click chemistry conjugation, linking its ethynyl group with the azido group of various fluorochromes.

H-(Gly)3-Lys(N3)-OH (hydrochloride) is a click chemistry reagent renowned for its high yield, specificity, and simplicity, making it an ideal choice for creating bindings among nucleic acids, lipids, proteins, and other molecules across various research domains.

3-Azidopropanol, a click chemistry reagent featuring an azide group, holds significant potential for facilitating bindings among nucleic acids, lipids, proteins, and various molecules. Its application across numerous research domains stems from advantageous traits like high yield, specificity, and simplicity [1].

3-Azidopropylamine, a click chemistry reagent characterized by its azide group, facilitates the complexation and transfection of plasmid DNA by reacting with the starch sugar present in potato starch [1].

Palmitoyl alkyne-coenzyme A (palmitoyl alkyne-CoA), a thioester formed from ω-alkynylated palmitic acid and CoA, serves as a chemical reporter for the labeling of palmitoylated proteins through click chemistry. This process utilizes probes such as biotin-azide or 3-azido-7-hydroxycoumarin for both in vitro and in vivo studies.

Palmitoleic acid alkyne, an ω-terminal alkyne derivative of palmitoleic acid, facilitates click chemistry applications. This compound has played a crucial role in examining protein palmitoylation processes. Specifically, the cis form of palmitoleic acid alkyne selectively tags wild-type Wnt3a protein within mouse fibroblast L-cells expressing Wnt3a and its secretion in conditioned media, distinguishing itself from the trans form and proving ineffective against the S209A mutant Wnt3a.

Amino-bis-PEG3-BCN, a cleavable 3-unit PEG ADC linker, is employed in the synthesis of antibody-drug conjugates (ADCs) [1]. It acts as a click chemistry reagent and features a BCN group that participates in strain-promoted alkyne-azide cycloaddition (SPAAC) with azide group-containing molecules.

Bocaminooxyacetamide-PEG3-alkyne, a cleavable 3-unit PEG ADC linker, plays a crucial role in the synthesis of antibody-drug conjugates (ADCs) [1]. This compound also functions as a click chemistry reagent due to its Alkyne group, enabling it to undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with Azide-containing molecules.

DBCO-PEG3 acetic-EVCit-PAB, a cleavable 3-unit PEG ADC linker, plays a crucial role in the synthesis of antibody-drug conjugates (ADCs) [1]. This compound features a DBCO group, enabling it to engage in strain-promoted alkyne-azide cycloaddition (SPAAC) with azide-bearing molecules, and is also utilized as a click chemistry reagent.

TCO-PEG3-CH2-aldehyde, a cleavable 3-unit PEG ADC linker, is employed in the construction of antibody-drug conjugates (ADCs) [1]. This compound serves as a click chemistry reagent and features a TCO group capable of participating in an inverse electron demand Diels-Alder reaction (iEDDA) with molecules that contain Tetrazine groups.

SCO-PEG7-Maleimide, a cleavable ADC linker with 3 PEG units, serves as a copper-free click chemical reagent for catalyst-free click reactions. Its maleimide group (-Maleimide) degrades in aqueous media and has applications in drug delivery studies.

BCN-exo-PEG3-maleimide, an ADC linker containing three PEG units, includes the lyophilic bidentate macrocyclic ligand BCN, facilitating the synthesis of macrocyclic complexes. In click chemistry, BCN reacts with azide-containing molecules to form stable triazoles without catalysts. The maleimide group (-Maleimide) degrades in aqueous media and has applications in drug delivery studies.

SCO-PEG3-NH2, a cleavable ADC linker with 3 PEG units, serves as a copper-free click chemical reagent for catalyst-free click reactions.

Mal-bis-PEG3-DBCO, a cleavable three-unit PEG ADC linker, is employed in the synthesis of antibody-drug conjugates (ADCs) [1]. This click chemistry reagent features a DBCO group that engages in strain-promoted alkyne-azide cycloaddition (SPAAC) with azide-containing molecules.

DBCO-PEG3-propionic EVCit-PAB is a 3-unit PEG ADC linker utilized in the production of antibody-drug conjugates (ADCs) [1]. As a click chemistry reagent, this compound features a DBCO group designed for strain-promoted alkyne-azide cycloaddition (SPAAC) with azide-containing molecules.

TCO-PEG3-aldehyde, a cleavable 3-unit PEG ADC linker, is used in the synthesis of antibody-drug conjugates (ADCs) [1]. This click chemistry reagent contains a TCO group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing Tetrazine groups.

Amino-bis-PEG3-DBCO serves as a cleavable 3-unit PEG ADC linker utilized in the creation of antibody-drug conjugates (ADCs) [1]. This compound is a click chemistry reagent featuring a DBCO group capable of participating in strain-promoted alkyne-azide cycloaddition (SPAAC) with Azide group-containing molecules.