5-fam

5-FAM (5-Carboxyfluorescein) (5-Carboxyfluorescein) contains a carboxylic acid, which can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its excellent fluorescence quantum yield, relatively high absorptivity, and good water solubility, it also has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.

5-FAM SE is a single isomer widely employed in the preparation of various small fluorescent molecules. It is a highly popular green fluorescent reagent used for labeling peptides, proteins, and nucleotides [5-FAM SE].

5-FAM maleimide, a derivative of 5-FAM, can be utilized for protein labeling [1].

5-FAM-Ahx-LL-37 TFA is a 5-FAM labeled version of LL-37, human. The carboxyfluorescein moiety is attached via 6-Aminohexanoic acid (Ahx). LL-37, human, is a 37-residue amphipathic antimicrobial peptide, derived from an antibiotic, possessing broad-spectrum antimicrobial activity.

5-FAM-LL-37 TFA is the TFA salt form of 5-FAM-LL-37. This fluorescein-labeled LL-37 peptide maintains the antimicrobial and immunomodulatory properties of LL-37. It binds to bacterial cell membranes, disrupting their integrity, and exhibits broad-spectrum antibacterial activity.

5-FAM-NH2.HCl is a derivative fluorescent dye of 5-FAM. 5-FAM (5-Carboxyfluorescein) is a green fluorescent probe and fluorescein derivative used for labelling peptides, proteins, and nucleic acids, with excitation/emission wavelengths of 490/520 nm.

5-FAM-Dyrktide is a Dyrktide compound labeled with 5-FAM. This substrate undergoes effective phosphorylation by DYRK1A (Km=35 μM) while remaining resistant to phosphorylation by ERK2.

5-FAM-PMDM6 (PMDM6-F) is a biologically active peptide. It serves as a fluorescently labeled probe used in MDM2 binding assays.

5-FAM-Alkyne is a green fluorescent probe (Ex/Em = 490 nm/520 nm), which can be used for labeling peptides, proteins, and nucleotides, and its Alkyne group can undergo click chemistry reactions with molecules containing Azide groups.

5-FAM-β-Amyloid (1-28) is a peptide labeled with a fluorescent probe, representing the initial 28 amino acids of the β-Amyloid protein.

5-FAM-LPETGG is a fluorescent peptide that combines the sortase recognition sequence LPETGG with the fluorescent label 5-carboxyfluorescein (5-FAM), characterized by excitation/emission maxima at 492/518 nm. It serves for site-specific labeling of target proteins through sortase-catalyzed transpeptidation.

5-FAM-LL-37 is a fluorescently labeled antimicrobial peptide conjugated to 5-carboxyfluorescein (5-FAM) through a 6-aminohexanoic acid (Ahx) linker, featuring excitation/emission maxima at 492/518 nm, respectively. This compound integrates the functional attributes of LL-37 with the fluorescent properties of 5-FAM for enhanced visualization.

5-FAM-GpYLPQTV-NH2, a fluorescently labeled peptide, exhibits STAT3 inhibitory activity and is applicable in cancer research [1].

Fura-5F AM is a membrane-permeable fluorescent calcium indicator. Once inside the cell, this probe is hydrolyzed by cellular esterases and acts as an active chelating agent.

5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.

5(6)-FAM SE (5(6)-Carboxyfluorescein N-hydroxysuccinimide ester) is an amine-reactive green fluorescent dye. 5(6)-FAM SE is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

6-ROX (6-Carboxy-X-rhodamine) is a fluorescent oligonucleotide marker and acts as an acceptor molecule coupled to 5-FAM as the donor in FRET imaging with excitation of 514nm and emission of 525nm.

FAM amine, 5-isomer is a fluorescein linker capable of undergoing enzymatic transamination reactions. The addition of an amine group enhances the compound's reactivity with carboxylic acids, activated NHS esters, and other carbonyl groups.

FAM tetrazine, 5-isomer, is a versatile dye featuring a terminal tetrazine group. The terminal methyl tetrazine group can undergo a rapid click reaction with TCO (trans-cyclooctene).

FAM hydrazide, 5-isomer is a fluorescein dye linker that contains a hydrazide functional group. Fluorescein is a versatile dye, and the hydrazide moiety reacts with aldehydes to form a semi-permanent hydrazone bond.

FAM-sgc8c aptamer (sodium) is a 5-FAM labeled sgc8c aptamer (sodium), which specifically targets human protein tyrosine kinase PTK-7, predominantly expressed on cervical cancer Hela cells. The binding of sgc8c aptamer sodium to PTK-7 induces the assembly of nanolabels on the cell surface, resulting in strong fluorescence and SERS signals with specificity for cervical cancer cells.

FAM azide, 5-isomer, is a reagent used for Click Chemistry labeling.

FAM phosphoramidite, 5-isomer, is a standard fluorescein (FAM) phosphoramidite used for 5' end labeling of oligonucleotides with a high-purity single isomer.

5-diacetate FAM NHS (5-CFDA SE) is a cell-permeable fluorescent tracer for use in fluorescence microscopy and flow cytometry, Ex=492 nm, Em=517 nm.