4methylumbelliferyl 2sulfamino2deoxyαdglucopyranoside (sodium salt)

4-Methylumbelliferyl 2-sulfamino-2-deoxy-α-D-Glucopyranoside (4-MU-α-GlcNS) is a fluorogenic substrate of heparin sulphamidase. Heparin sulphamidase cleaves 4-MU-α-GlcNS to yield 4-MU-α-GlcNH2, which is then cleaved by α-glucosaminidase to release the fluorescent product 4-MU, which displays an emission maxima of 445-454 nm. The excitation maxima for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. 4-MU-α-GlcNS has been used to quantify heparin sulphamidase deficiencies associated with Mucopolisaccaridosis IIIA and other lysosomal disorders.

2-(4-Hydroxyphenylazo)benzoicacid is a spectrophotometric probe that shows changes in absorption spectra upon binding to proteins and binds to bovine serum proteins.

6-Ethyl-7-hydroxy-4-methylchromen-2-one is a fluorescent dye.

2-(7-Hydroxy-2-oxo-2H-chromen-4-yl)acetic acid is a useful organic compound for research related to life sciences. The catalog number is T65277 and the CAS number is 6950-82-9.

Mag-Fluo-4 AM is a Mg 2+ (magnesium ion) dye and a low-affinity Ca 2+ (calcium ion) dye and fluorescent indicator with Kd of 4.7 mM and 22 µM, respectively, that localizes Ca 2+ in the lumen of endoplasmic reticulum-like tubules in many cell types, and is commonly used in live cell assays.

2-(7-Methoxy-2-oxo-2H-chromen-4-yl)acetic acid is a useful organic compound for research related to life sciences. The catalog number is T64951 and the CAS number is 62935-72-2.

4-(Benzyloxy)-2-nitrophenol serves as a synthetic precursor for constructing fluorescent ion indicators [1].

N-[4-(2-Benzimidazolyl)phenyl]maleimide is a potent fluorescent probe with an excitation maximum at 315 nm and an emission maximum at 360 nm, suitable for the fluorometry of thiol compounds [1].

C.I. Mordant Green 17 is an azo dye [which has a molecular formula of C16H13N4NaO3S, or systematically named as Sodium 3-[(2-hydroxy-3-nitrophenyl)azo]-4-methoxybenzenesulfonate].

6,7-Dimethoxy-4-coumarinylacetic acid (2-(6,7-Dimethoxy-2-oxo-2H-chromen-4-yl)acetic acid) serves as a multifunctional dye with diverse applications in both scientific and traditional domains. This compound facilitates the observation and analysis of cell structures in biological experiments, enabling researchers to track biomolecules, assess cell functions, categorize cell types, identify biomolecules, investigate tissue pathology, and monitor microorganisms. Beyond its scientific utility, 6,7-Dimethoxy-4-coumarinylacetic acid is also employed in industries including textile dyeing, functional textile processing, food pigments, and dye-sensitized solar cells. Its broad utility spans from fundamental research to clinical diagnostics, highlighting its importance across multiple fields.

Z-(L-Arg)-AMC is a fluorogenic substrate for trypsin, cathepsin B, and cathepsin H.1,2Upon enzymatic cleavage by trypsin, cathepsin B, or cathepsin H, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify trypsin, cathepsin B, and cathepsin H activity. AMC displays excitation emission maxima of 340-360 440-460 nm, respectively. 1.Zimmerman, M., Ashe, B., Yurewicz, E.C., et al.Sensitive assays for trypsin, elastase, and chymotrypsin using new fluorogenic substratesAnal. Biochem.78(1)47-51(1977) 2.Brindley, P.J., Kalinna, B.H., Dalton, J.P., et al.Proteolytic degradation of host hemoglobin by schistosomesMol. Biochem. Parasitol.89(1)1-9(1997)

DAPI (dilactate), a blue fluorescent dye, primarily targets and binds to AT clusters within the minor groove of dsDNA, significantly enhancing fluorescence by about 20-fold upon binding. It is particularly effective in staining the nucleus, allowing for clear identification of the cell cycle phases, while it does not stain the cytoplasm. The dilactate form of DAPI exhibits greater water solubility compared to its dihydrochloride counterpart. [1] [2]

1) Non-toxicity: belong to flower-stem dye, easy to biodegrade, no carcinogenic toxicity. 2) High sensitivity: at least 100pg ssDNA or RNA can be detected, which is 25 ~ 100 times higher than EB staining. 3) High SIGNal-to-noise ratio: the sample fluorescence signal is strong, while the background signal is low. 4) Simple operation: like EB, the dye does not degrade in the prefabricated gel and electrophoresis process;The dyeing process after electrophoresis takes only 30 minutes without decoloring or washing, and can be observed directly with ultraviolet gel transmission or visible light transmission.

Compound 2f, chemically known as 4-Methyl-7-(2-nitrophenoxy)-2H-chromen-2-one, is a selenium alcohol fluorescent probe designed on the nucleophilic aromatic substitution mechanism. It exhibits selective recognition of selenols in neutral aqueous solutions without significant interference from biological thiols, amines, or alcohols. This compound is utilized for quantifying selenium content in selenoproteins and imaging the activity of endogenous selenols in living cells.

4-Methylumbelliferyl phosphate disodium (4-MUP), an anionic organophosphate, serves as a fluorogenic substrate for acid and alkaline phosphatases. Additionally, this compound acts as a simulant for nerve agents [1] [2] [3].

4-(2-Aminoethylamino)-7-(N,N-dimethylsulfamoyl)benzofurazan functions as a precolumn fluorescence derivatization reagent.

4-Di-1-ASP, a styryl dye, selectively stains glioma cells within live brain tissue, facilitating microscopic analysis of cell structure, viability, and processes including proliferation, endocytosis, cytokinesis, and phagocytosis. It also allows for the observation of mitochondrial structures in living cells. The dye exhibits green fluorescence when visualized under a microscope (λ ex λ em = 475 606 nm) [1] [2].

Z-Gly-Pro-Arg-4MβNA is a thrombin substrate that, when cleaved by thrombin, releases 4-methoxy-2-naphthylamine (4MβNA). The liberated 4MβNA reacts with 5-nitrosalicylaldehyde, forming an insoluble yellow fluorescent product, which indicates the site of thrombin activity [1].

Sulfo-Cy3-Tetrazine is a water-soluble cyanine fluorescent dye featuring a tetrazine group for click chemistry applications. It undergoes an inverse electron demand [4+2] cycloaddition with trans-cyclooctene or other strained olefins.

Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone (PCNPH) acts as a chromogenic substrate for peroxidase enzymes, producing a purple indamine dye when reacting with peroxidases and peroxides [1].

4-(2-Carboxyphenyl)-7-diethylamino-2-(7-diethylamino-2-oxochroman-3-yl)-chromylium perchlorate is an organic fluorescent dye that features a carboxyl functional group, with an excitation wavelength (λ ex) of approximately 650 nm and an emission wavelength (λ em) of around 690 nm [1].

Biotin-11-dUTP [tetra(triethylammonium) salt] [(2S,3S,4R,5R,6R)-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxy-6-(hydroxymethyl)-2-[(3aR,4S,6R,6aS)-2-oxohexahydro-2H-thieno[3,4-d]imidazol-4-yl]-tetrahydro-2H-furo[3,2-d][1,3,2]dioxaphosphorin-3-yl]phosphonate] is used as a fluorescent substitute for dTTP.

2-4-(2-Cyanoethyl)(2-phenylethyl)aminophenylazo-5-nitrobenzonitrile is characterized by its storage stability and its fluid, nonagglomerating dispersion properties as an azo dye [1].

10-(4-(Bis(2-acetoxyethyl)amino)phenyl)-BODIPY 505 515 serves as a fluorescent dye specifically for Cu 2+ ions [1].