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6-Ethyl-7-hydroxy-4-methylchromen-2-one is a fluorescent dye.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
25 mg | $32 | In Stock | |
50 mg | $44 | In Stock | |
100 mg | $65 | In Stock |
Description | 6-Ethyl-7-hydroxy-4-methylchromen-2-one is a fluorescent dye. |
Cell Research | 1. Solution preparation 1. Preparation of mother solution: Dissolve 6-ethyl-7-hydroxy-4-methylchromen-2-one in an appropriate solvent, such as PBS or DMSO, usually at a concentration of 1-10 mM. 2. Preparation of working solution: Dilute the mother solution with PBS/DMEM/H2O to a 1-10 μM working solution for later use. 2. Cell staining and imaging: 1. Add cell sample: Add the dissolved dye to the cell culture and incubate for a certain period of time (usually 30 minutes to 2 hours) so that the dye enters the cell and binds to the cell components. 2. Wash and observe under a microscope: After washing away the excess dye with PBS, observe the fluorescence signal of the cell under a fluorescence microscope. 3. Molecular probe experiment: 1. Labeling molecules or probes: React the dye with the target molecule (such as protein, DNA, etc.) for labeling. 2. Fluorescence detection: Detect the fluorescence signal of the target molecule through a fluorescence spectrometer or imaging system to analyze its properties or changes. IV. Drug screening experiment: 1. Treat cells with drugs: incubate the drugs with cell samples. 2. Add dye: after staining, observe the fluorescence changes of the drugs on cells. |
Alias | 6-ethyl-7-hydroxy-4-methylcoumarin |
Molecular Weight | 204.22 |
Formula | C12H12O3 |
Cas No. | 1484-73-7 |
Smiles | CCc1cc2c(C)cc(=O)oc2cc1O |
Relative Density. | 1.227 g/cm3 (Predicted) |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice./Shipping at ambient temperature. | ||||||||||||||||||||
Solubility Information | DMSO: < 2.04 mg/mL (10 mM, insoluble or slightly soluble) ![]() | ||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||
DMSO
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