6-Ethyl-7-hydroxy-4-methylchromen-2-one is a fluorescent dye.

1. Solution preparation
1. Preparation of mother solution: Dissolve 6-ethyl-7-hydroxy-4-methylchromen-2-one in an appropriate solvent, such as PBS or DMSO, usually at a concentration of 1-10 mM.
2. Preparation of working solution: Dilute the mother solution with PBS/DMEM/H2O to a 1-10 μM working solution for later use.
2. Cell staining and imaging:
1. Add cell sample: Add the dissolved dye to the cell culture and incubate for a certain period of time (usually 30 minutes to 2 hours) so that the dye enters the cell and binds to the cell components.
2. Wash and observe under a microscope: After washing away the excess dye with PBS, observe the fluorescence signal of the cell under a fluorescence microscope.
3. Molecular probe experiment:
1. Labeling molecules or probes: React the dye with the target molecule (such as protein, DNA, etc.) for labeling.
2. Fluorescence detection: Detect the fluorescence signal of the target molecule through a fluorescence spectrometer or imaging system to analyze its properties or changes.
IV. Drug screening experiment:
1. Treat cells with drugs: incubate the drugs with cell samples.
2. Add dye: after staining, observe the fluorescence changes of the drugs on cells.